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69025 T7•Tag® Affinity Purification Kit

Immunoaffinity purification of T7•Tag fusion proteins

T7•Tag® Affinity Purification Kit MSDS (material safety data sheet) or SDS, CoA and CoQ, dossiers, brochures and other available documents.
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69025
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Overview

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      Description
      OverviewThe T7•Tag® Affinity Purification Kit is designed for rapid immunoaffinity purification of target proteins that carry the T7•Tag sequence (e.g., the 11 amino terminal aa of the T7 gene 10 protein). Purification is based on binding target proteins to T7•Tag Monoclonal Antibody that is covalently coupled to cross-linked agarose beads, washing away unbound proteins, and eluting at pH 2.2. Neutralization Buffer is included to limit protein exposure to low pH. Capacity will vary somewhat between different target proteins, but the beads are standardized to bind a minimum of 300 µg T7•Tag β-galactosidase per milliliter of settled resin. The beads can be used in either batch or column methods and can be regenerated a minimum of five times without loss of binding activity.
      Catalogue Number69025
      Brand Family Novagen®
      References
      Product Information
      Components
      Quality LevelMQ400
      Applications
      Biological Information
      Physicochemical Information
      Dimensions
      Materials Information
      Toxicological Information
      Safety Information according to GHS
      Safety Information
      R PhraseR: 36/37/38

      Irritating to eyes, respiratory system and skin.
      S PhraseS: 26-36

      In case of contact with eyes, rinse immediately with plenty of water and seek medical advice.
      Wear suitable protective clothing.
      Product Usage Statements
      Storage and Shipping Information
      Ship Code Blue Ice Only
      Toxicity Multiple Toxicity Values, refer to MSDS
      Storage +2°C to +8°C
      Do not freeze Yes
      Packaging Information
      Transport Information
      Supplemental Information
      Specifications
      Global Trade Item Number
      Catalogue Number GTIN
      69025-3 04055977274707

      Documentation

      T7•Tag® Affinity Purification Kit SDS

      Title

      Safety Data Sheet (SDS) 

      T7•Tag® Affinity Purification Kit Certificates of Analysis

      TitleLot Number
      69025

      Citations

      Title
    • Hayakawa, H., et al. 2007. Soluble Journal of Biological Chemistry 282, 26369.
    • Chang-Sik Oh and Steven V. Beer. 2007. Plant Physiology 145, 426.
    • Sukumar, N., et al. 2007. Journal of Bacteriology 189, 3695.
    • Qiu, R., et al. 2005. Journal of Biological Chemistry 280, 16695.
    • Suzuki, T., et al. 2005. Plant and Cell Physiology 46, 1452.
    • Michael H. Kagey, Tiffany A. Melhuish and David Wotton. 2003. The Cell 113, 127.
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      User Protocols

      Title
      TB055 pET System Manual
      TB125 T7•Tag® Affinity Purification Kit