Our broad portfolio consists of multiplex panels that allow you to choose, within the panel, analytes that best meet your needs. On a separate tab you can choose the premixed cytokine format or a single plex kit.
Cell Signaling Kits & MAPmates™
Choose fixed kits that allow you to explore entire pathways or processes. Or design your own kits by choosing single plex MAPmates™, following the provided guidelines.
The following MAPmates™ should not be plexed together:
-MAPmates™ that require a different assay buffer
-Phospho-specific and total MAPmate™ pairs, e.g. total GSK3β and GSK3β (Ser 9)
-PanTyr and site-specific MAPmates™, e.g. Phospho-EGF Receptor and phospho-STAT1 (Tyr701)
-More than 1 phospho-MAPmate™ for a single target (Akt, STAT3)
-GAPDH and β-Tubulin cannot be plexed with kits or MAPmates™ containing panTyr
.
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To begin designing your MILLIPLEX® MAP kit select a species, a panel type or kit of interest.
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48-602MAG
Buffer Detection Kit for Magnetic Beads
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Space Saver Option Customers purchasing multiple kits may choose to save storage space by eliminating the kit packaging and receiving their multiplex assay components in plastic bags for more compact storage.
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553506
Sigma-AldrichRAP, Human, Recombinant, E. coli
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Description
Overview
Recombinant, human RAP fused to GST, expressed in E. coli, and subsequently cleaved with thrombin to remove GST. Antagonizes the ligand binding of certain members of the low-density lipoprotein receptor family such as low density lipoprotein receptor-related protein (LRP) and very low-density lipoprotein (VLDL) receptor. Promotes the proper folding and subsequent exocytic trafficking of the wild-type low density lipoprotein receptor (LDLR) and several of its class 2 mutants. Expressed as a GST-fusion protein that is subsequently cleaved with thrombin. Typical concentrations to block LRP or VLDL receptor are 200-300 nM. For antagonism of LDLR, 1 µM is recommended.
Catalogue Number
553506
Brand Family
Calbiochem®
Synonyms
Receptor Associated Protein
References
References
Li, Y., et al. 2002. Biochemistry41, 4921. Salicioni, A.M., et al. 2002. J. Biol. Chem.277, 16160. Anderson, O.M., et al. 2001. Biochemistry40, 15408. Bu, G. 2001. Int. Rev. Cytol.209, 79. Williams, S.E., et al. 1992. J. Biol. Chem.267, 9035
RAP, Human, Recombinant, E. coli Certificates of Analysis
Title
Lot Number
553506
References
Reference overview
Li, Y., et al. 2002. Biochemistry41, 4921. Salicioni, A.M., et al. 2002. J. Biol. Chem.277, 16160. Anderson, O.M., et al. 2001. Biochemistry40, 15408. Bu, G. 2001. Int. Rev. Cytol.209, 79. Williams, S.E., et al. 1992. J. Biol. Chem.267, 9035
Data Sheet
Note that this data sheet is not lot-specific and is representative of the current specifications for this product. Please consult the vial label and the certificate of analysis for information on specific lots. Also note that shipping conditions may differ from storage conditions.
Revision
20-April-2009 JSW
Synonyms
Receptor Associated Protein
Description
Recombinant, human RAP fused to GST, expressed in E. coli, and subsequently cleaved with thrombin to remove GST. Receptor Associated Protein (RAP) antagonizes the ligand binding of certain members of the low-density lipoprotein receptor family such as low-density lipoprotein receptor-related protein (LRP) and very low density lipoprotein (VLDL) receptor. Promotes the proper folding and subsequent exocytic trafficking of the wild-type low-density lipoprotein receptor (LDLR) and several of its class 2 mutants. Typical concentrations used to block LRP or VLDL receptor are 200-300 nM; for antagonism of LDLR, 1 µM is recommended.
Form
Liquid
Formulation
150 mM NaCl, 100 mM Tris-HCl, pH 7.4.
Concentration Label
Please refer to vial label for lot-specific concentration
Purity
≥90% by SDS-PAGE
Storage
Avoid freeze/thaw ≤ -70°C
Do Not Freeze
Ok to freeze
Special Instructions
Following initial thaw, aliquot and freeze (-70°C).
Toxicity
Standard Handling
References
Li, Y., et al. 2002. Biochemistry41, 4921. Salicioni, A.M., et al. 2002. J. Biol. Chem.277, 16160. Anderson, O.M., et al. 2001. Biochemistry40, 15408. Bu, G. 2001. Int. Rev. Cytol.209, 79. Williams, S.E., et al. 1992. J. Biol. Chem.267, 9035