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540222 Puromycin, Dihydrochloride - CAS 58-58-2 - Calbiochem

Puromycin, Dihydrochloride, CAS 58-58-2, is An aminonucleoside antibiotic that inhibits protein synthesis by blocking the translation step and causes premature release of nascent polypeptide chains.

MSDS (material safety data sheet) or SDS, CoA and CoQ, dossiers, brochures and other available documents.

Synonyms: 3ʹ-(α-Amino-p-methoxyhydrocinnamamido)-3ʹ-deoxy-N,N-dimethyladenosine, 2HCl

Primary Target: protein synthesis
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      Overview

      Replacement Information

      Key Spec Table

      CAS #Empirical Formula
      58-58-2C₂₂H₂₉N₇O₅ · 2HCl
      Description
      OverviewProtein synthesis inhibitor. Inhibits translation by causing premature release of nascent polypeptide chains. Induces DNA fragmentation in thymocytes and in human HL-60 leukemia cells.
      Catalogue Number540222
      Brand Family Calbiochem®
      Synonyms3ʹ-(α-Amino-p-methoxyhydrocinnamamido)-3ʹ-deoxy-N,N-dimethyladenosine, 2HCl
      References
      ReferencesChow, S.C., et al. 1995. Exp. Cell Res. 216, 149.
      Constam, D.B., et al. 1995. J. Biol. Chem. 270, 26931.
      Wirth, M., et al. 1994. J. Virol. 68, 566.
      Claeyssens, S., et al. 1993. FEBS Lett. 315, 7.
      Hechler, U., et al. 1993. Biochem. Biophys. Res. Commun. 194, 1305.
      Kaufman, S.H., et al. 1993. Cancer Res. 53, 3976.
      de la Luna, S. and Ortín, J. 1992. Methods Enzymol. 216, 376.
      Kalpaxis, D.L. and Drainas, D. 1992. Arch. Biochem. Biophys. 300, 629.
      Shipston, M.J. and Antoni, F.A. 1992. Biochem. Biophys. Res. Commun. 189, 1382.
      Product Information
      CAS number58-58-2
      FormWhite to off-white solid
      Hill FormulaC₂₂H₂₉N₇O₅ · 2HCl
      Chemical formulaC₂₂H₂₉N₇O₅ · 2HCl
      Structure formula ImageStructure formula Image
      Quality LevelMQ100
      Applications
      Biological Information
      Primary Targetprotein synthesis
      Purity≥98% by TLC
      Physicochemical Information
      Dimensions
      Materials Information
      Toxicological Information
      Safety Information according to GHS
      RTECSAU7355000
      Safety Information
      Product Usage Statements
      Storage and Shipping Information
      Ship Code Ambient Temperature Only
      Toxicity Harmful
      Storage -20°C
      Protect from Moisture Protect from moisture
      Do not freeze Ok to freeze
      Special InstructionsFollowing reconstitution, sterilize by filtration through a 0.22 µm pore-size filter, aliquot and freeze (-20°C). Stock solutions are stable for up to 3 months at -20°C.
      Packaging Information
      Transport Information
      Supplemental Information
      Specifications
      Global Trade Item Number
      Catalogue Number GTIN
      540222 0

      Documentation

      Puromycin, Dihydrochloride - CAS 58-58-2 - Calbiochem SDS

      Title

      Safety Data Sheet (SDS) 

      Puromycin, Dihydrochloride - CAS 58-58-2 - Calbiochem Certificates of Analysis

      TitleLot Number
      540222

      References

      Reference overview
      Chow, S.C., et al. 1995. Exp. Cell Res. 216, 149.
      Constam, D.B., et al. 1995. J. Biol. Chem. 270, 26931.
      Wirth, M., et al. 1994. J. Virol. 68, 566.
      Claeyssens, S., et al. 1993. FEBS Lett. 315, 7.
      Hechler, U., et al. 1993. Biochem. Biophys. Res. Commun. 194, 1305.
      Kaufman, S.H., et al. 1993. Cancer Res. 53, 3976.
      de la Luna, S. and Ortín, J. 1992. Methods Enzymol. 216, 376.
      Kalpaxis, D.L. and Drainas, D. 1992. Arch. Biochem. Biophys. 300, 629.
      Shipston, M.J. and Antoni, F.A. 1992. Biochem. Biophys. Res. Commun. 189, 1382.

      Brochure

      Title
      Antibiotics Profiler
      Caspases and other Apoptosis Related Tools Brochure
      Data Sheet

      Note that this data sheet is not lot-specific and is representative of the current specifications for this product. Please consult the vial label and the certificate of analysis for information on specific lots. Also note that shipping conditions may differ from storage conditions.

      Revision16-May-2008 RFH
      Synonyms3ʹ-(α-Amino-p-methoxyhydrocinnamamido)-3ʹ-deoxy-N,N-dimethyladenosine, 2HCl
      DescriptionProkaryotic and eukaryotic protein synthesis inhibitor. Nucleoside that resembles the aminoacyl-adenylyl terminus of aminoacyl-tRNA and competes for binding to the "A site" of the large ribosomal subunit. Its incorporation into a growing polypeptide results in termination of chain elongation and release of the nascent polypeptidyl-puromycin. Used for selection of mammalian cell lines expressing a transfected pac gene (Streptomyces alboniger), whose product, puromycin acetyltransferase, inactivates the drug via acetylation. Induces DNA fragmentation in thymocytes and in human HL-60 leukemia cells. Like cycloheximide, puromycin is also used as a general protein synthesis inhibitor in numerous cellular assays.
      FormWhite to off-white solid
      CAS number58-58-2
      RTECSAU7355000
      Chemical formulaC₂₂H₂₉N₇O₅ · 2HCl
      Structure formulaStructure formula
      Purity≥98% by TLC
      SolubilityH₂O (50 mg/ml) and Methanol (20 mg/ml)
      Storage Protect from moisture
      -20°C
      Do Not Freeze Ok to freeze
      Special InstructionsFollowing reconstitution, sterilize by filtration through a 0.22 µm pore-size filter, aliquot and freeze (-20°C). Stock solutions are stable for up to 3 months at -20°C.
      Toxicity Harmful
      Merck USA index14, 7943
      ReferencesChow, S.C., et al. 1995. Exp. Cell Res. 216, 149.
      Constam, D.B., et al. 1995. J. Biol. Chem. 270, 26931.
      Wirth, M., et al. 1994. J. Virol. 68, 566.
      Claeyssens, S., et al. 1993. FEBS Lett. 315, 7.
      Hechler, U., et al. 1993. Biochem. Biophys. Res. Commun. 194, 1305.
      Kaufman, S.H., et al. 1993. Cancer Res. 53, 3976.
      de la Luna, S. and Ortín, J. 1992. Methods Enzymol. 216, 376.
      Kalpaxis, D.L. and Drainas, D. 1992. Arch. Biochem. Biophys. 300, 629.
      Shipston, M.J. and Antoni, F.A. 1992. Biochem. Biophys. Res. Commun. 189, 1382.