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70123 Protein Refolding Kit

Simple solubilization and refolding of inclusion body proteins

Protein Refolding Kit MSDS (material safety data sheet) or SDS, CoA and CoQ, dossiers, brochures and other available documents.
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      Overview

      Replacement Information
      Description
      Overview

      This product has been discontinued.





      The Protein Refolding Kit provides the basic reagents to solubilize and refold recombinant proteins that accumulate as inclusion bodies in E. coli by a patented procedure (Burgess 1996, Frankel 1993).
      Catalogue Number70123
      Brand Family Novagen®
      Features and benefits
      • Provides for easy isolation and solubilization of inclusion bodies under mild denaturing conditions.
      • Solubilized fraction is dialyzed against a neutral pH buffer containing a reducing agent to encourage correct disulfide bond formation.
      • A second dialysis step removes excess reducing agent and transfers the protein into the buffer of choice
      • Additional separate components such as NDSBs can be added to increase refolding efficiency of individual proteins.
      • Sufficient buffers are provided to process up to 2 g inclusion bodies.
      References
      ReferencesBurgess, R.R. 1996. Meth. Enzymol. 273, 145.
      Frankel, S.A. and Leinwand, L.A. 1993. U.S. Patent No. 5,240,834.
      Product Information
      •100 ml10X IB Wash Buffer
      •50 ml10X IB Solubilization Buffer
      •2 x 100 ml50X Dialysis Buffer
      •2 x 1.6 ml1M DTT
      •10 ml30% N-lauroylsarcosine
      Applications
      Biological Information
      Physicochemical Information
      Dimensions
      Materials Information
      Toxicological Information
      Safety Information according to GHS
      Safety Information
      Product Usage Statements
      Storage and Shipping Information
      Ship Code Blue Ice Only
      Toxicity Multiple Toxicity Values, refer to MSDS
      Storage +2°C to +8°C
      Avoid freeze/thaw Avoid freeze/thaw
      Do not freeze Ok to freeze
      Packaging Information
      Transport Information
      Supplemental Information
      Specifications
      Global Trade Item Number
      Catalogue Number GTIN
      70123 0

      Documentation

      Protein Refolding Kit Certificates of Analysis

      TitleLot Number
      70123

      References

      Reference overview
      Burgess, R.R. 1996. Meth. Enzymol. 273, 145.
      Frankel, S.A. and Leinwand, L.A. 1993. U.S. Patent No. 5,240,834.

      Citations

      Title
    • Y. S. Kim, et al. (2007) Production of a polyclonal anti-myostatin antibody and the effects of in ovo administration of the antibody on posthatch broiler growth and muscle mass. Poultry Science 86, 1196-1205.
    • Ke Yao, et al. (2007) Nuclear factor of activated T3 is a negative regulator of Ras-JNK1/2-AP-1-induced cell transformation. Cancer Research 67, 8725-8735.
    • Jean-Michel Heraud, et al. (2006) Subunit recombinant vaccine protects against monkeypox. Journal of immunology 177, 2552-2564.
    • Kai-Fai Lee, et al. (2006) Demilune cell and parotid protein from murine oviductal epithelium stimulates preimplantation embryo development. Endocrinology 147, 79-87.
    • James L. Stafford, et al. (2006) Identification and characterization of a FcR homolog in an ectothermic vertebrate, the channel catfish (Ictalurus punctatus). Journal of Immunology 177, 2505-2517.
    • Thomas H. Barker, et al. (2005) SPARC regulates extracellular matrix organization through its modulation of integrin-linked kinase activity. Journal of Biological Chemistry 280, 36483-36493.
    • Jonathan M. Houseley, et al. (2005) Myotonic dystrophy associated expanded CUG repeat muscleblind positive ribonuclear foci are not toxic to Drosophila. Human Molecular Genetics 14, 873-883.
    • Catherine Paradis-Bleau, Francois Sanschagrin and Roger C. Levesque. (2005) Peptide inhibitors of the essential cell division protein FtsA. Protein Engineering Design and Selection 18, 85-91.
    • Heinz C. Schroeder, et al. (2003) Emergence and disappearance of an immune molecule, an antimicribioal lectin, in basal metazoa. Journal of Biological Chemistry 278, 32810-32817.
    • Philip J. Young, et al. (2003) The Ewing's sarcoma protein interacts with the Tudor domain of the survival motor neuron protein. Molecular Brain Research 119, 37-49.
    • Claudia Naumann, Thomas Hartmann and Dietrich Ober. (2002) Evolutionary recruitment of a flavin-dependent monooxygenase for the detoxification of host plant-acquired pyrrolizidine alkaloids in the alkaloid-defended arctiid moth Tyria jacobaeae. Procedings of the National Academy of Science 99, 6085-6090.
    • Daniela Barilla, Barbara A. Lee and Nick J. Proudfoot. (2001) Cleavage/ polyadenylation factor IA associates with the carboxyl-terminal domain of RNA polymerase II in Saccharomyces cerevisiae. Procedings of the National Academy of Science 98, 445-450.
    • Tomoharu Okubo, et al. (2001) Down-regulation of promoter I.3 activity of the human aromatase gene in breast tissue by zinc-finger protein, Snail (SnaH). Cancer Research 61, 1338-1346.
    • Sujata Persad, et al. (2001) Regulation of protein kinase B/ Akt-serine 473 phosphorylation by integrin-linked kinase. Journal of Biological Chemistry 276, 27462-27469.
    • Jen-Wen Shiau, et al. (2001) Mice immunized with DNA encoding a modified Pseudomonas aeruginosa exotoxin A develop protective immunity against exotoxin intoxication. Vaccine 19, 1106-1112.
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      User Protocols

      Title
      TB234 Protein Refolding Kit