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16-201 Protein G Agarose/Salmon Sperm DNA, 2.5 mL

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16-201
2.5 mL  2.5 mL packed beads provided as a 50% gel slurry for a final volume of 5 mL.
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      Overview

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      Achieve first time ChIP success with EZ-ChIP Assays
      Description
      Catalogue Number16-201
      Brand Family Upstate
      Trade Name
      • Upstate
      DescriptionProtein G Agarose/Salmon Sperm DNA, 2.5 mL
      OverviewRecombinant Protein G covalently bound to agarose by cyanogen bromide linkage. Sonicated salmon sperm DNA blocks non-specific DNA binding sites on protein G agarose when collecting immunocomplexes for chromatin immunoprecipitations (ChIP assays).
      Alternate Names
      • ChIP agarose beads
      • ChIP agaraose G beads
      Background InformationRecombinant protein G has a very high affinity for the Fc regions of IgG molecules. Immoblisation of protein G on beads creates an affinity resin which can be used to purify IgG fractions from crude serum, ascites fluid or cell cuture media.
      Product Information
      Presentation2.5 mL packed beads containing 1 mg sonicated salmon sperm DNA, 2.5 mg BSA and approximately 5 mg recombinant Protein G. Provided as a 50% gel slurry for a final volume of 5 mL per vial. Suspended in 10 mM Tris-HCl, 1 mM EDTA, pH 8.0, containing 0.05% sodium azide. Liquid suspension.
      Quality LevelMQ100
      Applications
      ApplicationRecombinant Protein G covalently bound to agarose beads by cyanogen bromide linkage for use in chromatin immunoprecipitations (ChIP assays). Sonicated salmon sperm DNA is included to block non-specific DNA binding sites on agarose beads. Every lot qualified using an Acetyl Histone H3 ChIP assay.
      Product Usage Statements
      Quality AssuranceRoutinely evaluated in Chromatin Immunoprecipitation for use to pre-clear the supernatant from sodium butyrate treated HeLa cells and subsequent immunoprecipitation of DNA cross-linked to acetylated histones
      Usage Statement
      • Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
      Storage and Shipping Information
      Storage Conditions1 year at 4°C from date of shipment.
      Packaging Information
      Material Size2.5 mL
      Material Package2.5 mL packed beads provided as a 50% gel slurry for a final volume of 5 mL.
      Global Trade Item Number
      Catalogue Number GTIN
      16-201 04053252326370