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AP1064 PhosphoDetect™ Anti-PDH-E1α (pSer³⁰⁰) Rabbit pAb

This PhosphoDetect™ Anti-PDH-E1α (pSer³⁰⁰) Rabbit pAb is validated for use in Immunoblotting, Immunocytochemistry for the detection of PDH-E1α (pSer³⁰⁰).

MSDS (material safety data sheet) or SDS, CoA and CoQ, dossiers, brochures and other available documents.

Synonyms: Anti-Pyruvate Dehydogenase pSer³⁰⁰ Rabbit pAb

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AP1064
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Overview

Replacement Information

Key Spec Table

Species ReactivityHostAntibody Type
HRbPolyclonal Antibody

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AP1064-50UG
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      Description
      Overview

      This product has been discontinued.



      Recognizes the ~44 kDa PDH-E1α protein phosphorylated at Ser300 in HEK293 cells.
      Catalogue NumberAP1064
      Brand Family Calbiochem®
      SynonymsAnti-Pyruvate Dehydogenase pSer³⁰⁰ Rabbit pAb
      Application Data
      Detection of human PDH-E1α, phosphorylated at Ser300, by immunoblotting. Samples: Whole cell lysate from HEK293 cells left untreated (Lane 1) or treated with dichloroacetate (Lane 2). Primary antibody: PhosphoDetect™ Anti-PDH-E1α (pSer300) Rabbit pAb (Cat. No. AP1064) (0.5 µg/ml). Detection: chemiluminescence.

      Detection of human PDH-E1α, phosphorylated at Ser300, by immunocytochemistry. Samples: HEK293 cells left untreated (top panels) or treated with dichloroacetate (lower panels). Primary antibody: PhosphoDetect™ Anti-PDH-E1α (pSer300) Rabbit pAb (Cat. No. AP1064) (1µg/ml). Detection: fluorescence with DAPI (blue, nuclei) and Alexa Fluor 488® dye-Phalloidin (green, actin filaments) counterstains.
      References
      ReferencesRardin M.J., et. al. 2009. Anal. Biochem. 2, 157.
      Seifert, F., et al. 2007. Biochemistry 21, 6277.
      Lee, J., et al. 2007. Mol. Cell Prot. 4, 669.
      Patel, M.S. and Korotchkina, L.G. 2006 Biochem. Soc. Trans. 34, 217.
      Korotchkina, L.G., et al. 2001. J. Biol. Chem. 40, 37223.
      Product Information
      FormLiquid
      FormulationIn 150 mM NaCl, 100 mM Tris-Glycine, pH 7.4.
      Negative controlHEK293 cells treated with dichloroacetate
      Positive controlHEK293 cells
      Preservative≤0.1% sodium azide
      Quality LevelMQ200
      Applications
      Key Applications Immunoblotting (Western Blotting)
      Immunocytochemistry
      Application NotesImmunoblotting (0.5 µg/ml)
      Immunocytochemistry (1 µg/ml)
      Application CommentsDue to a high degree of sequence identity, this antibody is also expected to cross-react with mouse, rat, bovine, Zebra fish, and Xenopus phospho-PDH-E1α Antibody should be titrated for optimal results in individual systems.
      Biological Information
      Immunogena synthetic phosphopeptide corresponding to amino acids surrounding the Ser³⁰⁰ phosphorylation site of human PDH-E1α
      ImmunogenHuman
      HostRabbit
      Species Reactivity
      • Human
      Antibody TypePolyclonal Antibody
      Concentration Label Please refer to vial label for lot-specific concentration
      Physicochemical Information
      Dimensions
      Materials Information
      Toxicological Information
      Safety Information according to GHS
      Safety Information
      Product Usage Statements
      Storage and Shipping Information
      Ship Code Blue Ice Only
      Toxicity Standard Handling
      Storage -20°C
      Avoid freeze/thaw Avoid freeze/thaw
      Do not freeze Ok to freeze
      Special InstructionsFollowing initial thaw, aliquot and freeze (-20°C).
      Packaging Information
      Transport Information
      Supplemental Information
      Specifications
      Global Trade Item Number
      Catalogue Number GTIN
      AP1064-50UG 04055977227802

      Documentation

      PhosphoDetect™ Anti-PDH-E1α (pSer³⁰⁰) Rabbit pAb SDS

      Title

      Safety Data Sheet (SDS) 

      PhosphoDetect™ Anti-PDH-E1α (pSer³⁰⁰) Rabbit pAb Certificates of Analysis

      TitleLot Number
      AP1064

      References

      Reference overview
      Rardin M.J., et. al. 2009. Anal. Biochem. 2, 157.
      Seifert, F., et al. 2007. Biochemistry 21, 6277.
      Lee, J., et al. 2007. Mol. Cell Prot. 4, 669.
      Patel, M.S. and Korotchkina, L.G. 2006 Biochem. Soc. Trans. 34, 217.
      Korotchkina, L.G., et al. 2001. J. Biol. Chem. 40, 37223.
      Data Sheet

      Note that this data sheet is not lot-specific and is representative of the current specifications for this product. Please consult the vial label and the certificate of analysis for information on specific lots. Also note that shipping conditions may differ from storage conditions.

      Revision11-October-2011 JSW
      SynonymsAnti-Pyruvate Dehydogenase pSer³⁰⁰ Rabbit pAb
      ApplicationImmunoblotting (0.5 µg/ml)
      Immunocytochemistry (1 µg/ml)
      Application Data
      Detection of human PDH-E1α, phosphorylated at Ser300, by immunoblotting. Samples: Whole cell lysate from HEK293 cells left untreated (Lane 1) or treated with dichloroacetate (Lane 2). Primary antibody: PhosphoDetect™ Anti-PDH-E1α (pSer300) Rabbit pAb (Cat. No. AP1064) (0.5 µg/ml). Detection: chemiluminescence.

      Detection of human PDH-E1α, phosphorylated at Ser300, by immunocytochemistry. Samples: HEK293 cells left untreated (top panels) or treated with dichloroacetate (lower panels). Primary antibody: PhosphoDetect™ Anti-PDH-E1α (pSer300) Rabbit pAb (Cat. No. AP1064) (1µg/ml). Detection: fluorescence with DAPI (blue, nuclei) and Alexa Fluor 488® dye-Phalloidin (green, actin filaments) counterstains.
      DescriptionImmunoaffinity purified rabbit polyclonal antibody. Recognizes the ~44 kDa PDH-E1α protein phosphorylated at Ser300.
      BackgroundThe pyruvate dehydogenase (PDH) complex (PDC) catalyzes the irreversible conversion of pyruvate to acetyl CoA, providing the link between glycolyis and the TCA cycle. Dysregulation of the PDC in skeletal muscle has been implicated in type 2 diabetes and mutations in the PDC result in various mitochondrial diseases. The PDC is a multienzyme complex composed of several copies of three catalytic proteins (E1, E2, and E3): one structural (E3-binding protein) and two regulatory proteins (PDH kinase [PDK] and PDH phosphatase [PDP]). The activity of the PDC is mainly regulated by phosphorylation-dephosphorylation of three specific serine residues (232, 293, 300) on the E1α subunit. Phosphorylation of PDH determines the activity of the complex. Phosphorylation by one of four PDK isoforms inactivates the complex, while removal of the phosphate by one of two PDP isoforms activates the complex. The activity of the PDP1 isoform is sensitive to Ca2+, while the PDK isoforms are regulated by the reduction and acetylation status of the E2 subunit, pyruvate, and the small molecule the anti cancer dichloroacetate. (DCA).
      HostRabbit
      Immunogen speciesHuman
      Immunogena synthetic phosphopeptide corresponding to amino acids surrounding the Ser³⁰⁰ phosphorylation site of human PDH-E1α
      Specieshuman
      Positive controlHEK293 cells
      Negative controlHEK293 cells treated with dichloroacetate
      FormLiquid
      FormulationIn 150 mM NaCl, 100 mM Tris-Glycine, pH 7.4.
      Concentration Label Please refer to vial label for lot-specific concentration
      Preservative≤0.1% sodium azide
      CommentsDue to a high degree of sequence identity, this antibody is also expected to cross-react with mouse, rat, bovine, Zebra fish, and Xenopus phospho-PDH-E1α Antibody should be titrated for optimal results in individual systems.
      Storage Avoid freeze/thaw
      -20°C
      Do Not Freeze Ok to freeze
      Special InstructionsFollowing initial thaw, aliquot and freeze (-20°C).
      Toxicity Standard Handling
      ReferencesRardin M.J., et. al. 2009. Anal. Biochem. 2, 157.
      Seifert, F., et al. 2007. Biochemistry 21, 6277.
      Lee, J., et al. 2007. Mol. Cell Prot. 4, 669.
      Patel, M.S. and Korotchkina, L.G. 2006 Biochem. Soc. Trans. 34, 217.
      Korotchkina, L.G., et al. 2001. J. Biol. Chem. 40, 37223.