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539568 PP2B, Human, Recombinant, E. coli

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539568
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539568-5000U
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      Description
      OverviewRecombinant, human calcineurin Aα (catalytic subunit) and calcineurin B (regulatory subunit) co-expressed with yeast myristoyl-CoA:protein N-myristoyltransferase. A Ca2+/CaM-dependent protein phosphatase. The resulting highly active calcineurin is N-myristoylated on the CaN B-subunit, as is the native protein. M.W. 60,000 (A subunit) and 15,000 (B subunit).
      Catalogue Number539568
      Brand Family Calbiochem®
      SynonymsCaN, Protein Phosphatase 2B, Calcineurin
      References
      ReferencesGuerini, D. 1997. Biochem. Biophys. Res. Commun. 235, 271.
      Mondragon, A., et al. 1997. Biochemistry 36, 4934.
      Wei, Q. and Lee, E.Y. 1997. Biochemistry 36, 7418.
      Product Information
      Activity≥50,000 units/ml
      Unit of DefinitionOne unit is defined as the amount of enzyme that will release 1.0 pmol of phosphate from the RII phosphopeptide substrate (DLDVPIPGRFDRRVpSVAAE; <a href ="/Products/ProductDisplay.asp→catNO=207008">Cat. No. 207008</a>) in the presence of calmodulin per min at 30°C, pH 7.4.
      FormLiquid
      FormulationIn 100 mM NaCl, 50 mM Tris, 6 mM MgCl₂, 5 mM DTT, 0.5 mM CaCl₂, 0.025% NP-40.
      Quality LevelMQ100
      Biological Information
      PurityTwo distinct bands by SDS-PAGE
      Specific Activity≥300,000 units/mg
      Storage and Shipping Information
      Ship Code Dry Ice Only
      Toxicity Standard Handling
      Storage ≤ -70°C
      Avoid freeze/thaw Avoid freeze/thaw
      Do not freeze Ok to freeze
      Special InstructionsFollowing initial thaw, aliquot and freeze (-70°C). Please refer to vial label for lot-specific reconstitution volume.
      Global Trade Item Number
      Catalogue Number GTIN
      539568-5000U 04055977194685