Millipore Sigma Vibrant Logo

33-040 PI 3-Kinase 3-step HTRF™ Assay

Everything you need to perform the PI3 Kinase 3-step HTRF assays (384 wells).

PI 3-Kinase 3-step HTRF™ Assay MSDS (material safety data sheet) or SDS, CoA and CoQ, dossiers, brochures and other available documents.
Key Applications: Kinase Assay
View Products on Sigmaaldrich.com
33-040
384 wells  Kit capacity: 384 wells
Price could not be retrieved
Minimum Quantity is a multiple of
Maximum Quantity is
Upon Order Completion More Information
You Saved ()
 
—
Request Pricing
—
Limited Availability Limited Availability
In Stock 
Discontinued
Limited Quantities Available
Availability to be confirmed
    Remaining : Will advise
      Remaining : Will advise
      Will advise
      Contact Customer Service
      Contact Customer Service

       

      Contact Customer Service

      Overview

      Key Spec Table

      Species ReactivityKey ApplicationsAnalytes AvailableDetection Methods
      H, MKAPI 3-Kinase HTRF
      Description
      Catalogue Number33-040
      Brand Family Upstate
      Trade Name
      • HTRF
      • Upstate
      DescriptionPI 3-Kinase 3-step HTRF™ Assay
      Product Information
      Components
      • 4X Reaction Buffer
      • PIP2 (substrate, 1mM stock)
      • DM A Reaction Buffer
      • DM B Reaction Buffer
      • DM C Reaction Buffer
      • BPIP3
      • Stop Reagent
      Detection methodHTRF
      Quality LevelMQ100
      Applications
      ApplicationEverything you need to perform the PI3 Kinase 3-step HTRF assays (384 wells).
      Key Applications
      • Kinase Assay
      Biological Information
      Species Reactivity
      • Human
      • Mouse
      Analytes Available
      • PI 3-Kinase
      Entrez Gene Number
      Entrez Gene SummaryPhosphatidylinositol 3-kinase is composed of an 85 kDa regulatory subunit and a 110 kDa catalytic subunit. The protein encoded by this gene represents the catalytic subunit, which uses ATP to phosphorylate PtdIns, PtdIns4P and PtdIns(4,5)P2. This gene has been found to be oncogenic and has been implicated in cervical cancers.
      Gene Symbol
      • PIK3CA
      • MGC142163
      • PI3K
      • MGC142161
      • p110-alpha
      Target Sub-FamilyPI3K/PI4K
      UniProt Number
      UniProt SummaryFUNCTION: SwissProt: P42336 # Phosphorylates PtdIns, PtdIns4P and PtdIns(4,5)P2 with a preference for PtdIns(4,5)P2.
      SIZE: 1068 amino acids; 124284 Da
      SUBUNIT: Heterodimer of a p110 (catalytic) and a p85 (regulatory) subunit. Binds to IRS1 in nuclear extracts. Interacts with RUFY3 (By similarity).
      DISEASE: SwissProt: P42336 # Defects in PIK3CA are associated with colorectal cancer (CRC) [MIM:114500]. & Defects in PIK3CA are associated with breast cancer [MIM:114480]. & Defects in PIK3CA are associated with epithelial ovarian cancer (EOC) [MIM:604370].
      SIMILARITY: SwissProt: P42336 ## Belongs to the PI3/PI4-kinase family. & Contains 1 C2 domain. & Contains 1 PI3K/PI4K domain.
      Product Usage Statements
      Usage Statement
      • Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
      Storage and Shipping Information
      Storage Conditions12 months at -70°C
      Packaging Information
      Material Size384 wells
      Material PackageKit capacity: 384 wells
      Global Trade Item Number
      Catalogue Number GTIN
      33-040 04053252854378