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444147 HCV NS3/4A Protease, Recombinant, E. coli

MSDS (material safety data sheet) or SDS, CoA and CoQ, dossiers, brochures and other available documents.

Synonyms: Hepatitis C Virus NS3/4A Protease, Recombinant, E. coli.

444147
  
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      Overview

      Replacement Information
      Description
      Overview

      This product has been discontinued.





      Recombinant, human HCV NS3/4A protease expressed in E. coli and purified by affinity chromatography using FPLC. HCV is a positive, single-stranded RNA virus of the Flaviviridae family that encodes a polyprotein precursor of about 3,000 amino acids. It is a common cause of chronic liver disease. HCV NS3/4A is an essential enzyme for viral replication. It is believed that HCV NS3/4A protease could play an important role in a treatment of hepatitis C infection.
      Catalogue Number444147
      Brand Family Calbiochem®
      SynonymsHepatitis C Virus NS3/4A Protease, Recombinant, E. coli.
      References
      ReferencesDe Francesco, R., et al. 2003. Antiviral Res. 58, 1.
      Lamarre, D., et al. 2003. Nature 426, 186.
      Kolykhalov, A. et al. 2000. J. Virol. 74, 2046.
      Blight, K. J., et al. 1998. Antiviral Ther. 3, 71.
      Alter, M. J. 1997. Hepatology 26, 62S.
      Bartenschlager, et al. 1995. J. Virol. 69, 7519.
      Lin, C., and Rice, C. M. 1995. Proc. Natl. Acad. Sci. U.S.A. 92, 7622.
      Grakoui, A., et al. 1993. J. Virol. 67, 2832.
      Hijikata, M., et al. 1993. Proc. Natl. Acad. Sci. U.S.A. 90, 10773.
      Tomei, L., et al. 1993. J. Virol. 67, 4017.
      Product Information
      Unit of DefinitionOne unit is defined as 1 ng of purified protein.
      FormLiquid
      FormulationIn 500 mM KCl, 20 mM Tris-HCl, 1 mM DTT, 0.2 mM EDTA, 20% glycerol, pH 8.0.
      Applications
      Biological Information
      Biological activity20-200 ng is sufficient for an in vitro protease assay
      Purity≥95% by SDS-PAGE
      Concentration Label Please refer to vial label for lot-specific concentration
      Physicochemical Information
      Dimensions
      Materials Information
      Toxicological Information
      Safety Information according to GHS
      Safety Information
      Product Usage Statements
      Storage and Shipping Information
      Ship Code Dry Ice Only
      Toxicity Standard Handling
      Storage ≤ -70°C
      Avoid freeze/thaw Avoid freeze/thaw
      Do not freeze Ok to freeze
      Special InstructionsFollowing initial thaw, aliquot and freeze (-70°C).
      Packaging Information
      Transport Information
      Supplemental Information
      Specifications
      Global Trade Item Number
      Catalogue Number GTIN
      444147 0

      Documentation

      References

      Reference overview
      De Francesco, R., et al. 2003. Antiviral Res. 58, 1.
      Lamarre, D., et al. 2003. Nature 426, 186.
      Kolykhalov, A. et al. 2000. J. Virol. 74, 2046.
      Blight, K. J., et al. 1998. Antiviral Ther. 3, 71.
      Alter, M. J. 1997. Hepatology 26, 62S.
      Bartenschlager, et al. 1995. J. Virol. 69, 7519.
      Lin, C., and Rice, C. M. 1995. Proc. Natl. Acad. Sci. U.S.A. 92, 7622.
      Grakoui, A., et al. 1993. J. Virol. 67, 2832.
      Hijikata, M., et al. 1993. Proc. Natl. Acad. Sci. U.S.A. 90, 10773.
      Tomei, L., et al. 1993. J. Virol. 67, 4017.
      Data Sheet

      Note that this data sheet is not lot-specific and is representative of the current specifications for this product. Please consult the vial label and the certificate of analysis for information on specific lots. Also note that shipping conditions may differ from storage conditions.

      Revision16-June-2008 RFH
      SynonymsHepatitis C Virus NS3/4A Protease, Recombinant, E. coli.
      DescriptionRecombinant, human HCV NS3/4A protease expressed in E. coli and purified by affinity chromatography using FPLC. HCV is a positive, single-stranded RNA virus in the Flaviviridae family that encodes a polyprotein precursor of about 3,000 amino acids. It is a common cause of chronic liver disease. HCV NS3/4A is an essential enzyme for viral replication. It is believed that HCV NS3/4A protease could play an important role in a treatment of hepatitis C patients.
      FormLiquid
      FormulationIn 500 mM KCl, 20 mM Tris-HCl, 1 mM DTT, 0.2 mM EDTA, 20% glycerol, pH 8.0.
      Concentration Label Please refer to vial label for lot-specific concentration
      Purity≥95% by SDS-PAGE
      Biological activity20-200 ng is sufficient for an in vitro protease assay
      Unit definitionOne unit is defined as 1 ng of purified protein.
      Storage Avoid freeze/thaw
      ≤ -70°C
      Do Not Freeze Ok to freeze
      Special InstructionsFollowing initial thaw, aliquot and freeze (-70°C).
      Toxicity Standard Handling
      ReferencesDe Francesco, R., et al. 2003. Antiviral Res. 58, 1.
      Lamarre, D., et al. 2003. Nature 426, 186.
      Kolykhalov, A. et al. 2000. J. Virol. 74, 2046.
      Blight, K. J., et al. 1998. Antiviral Ther. 3, 71.
      Alter, M. J. 1997. Hepatology 26, 62S.
      Bartenschlager, et al. 1995. J. Virol. 69, 7519.
      Lin, C., and Rice, C. M. 1995. Proc. Natl. Acad. Sci. U.S.A. 92, 7622.
      Grakoui, A., et al. 1993. J. Virol. 67, 2832.
      Hijikata, M., et al. 1993. Proc. Natl. Acad. Sci. U.S.A. 90, 10773.
      Tomei, L., et al. 1993. J. Virol. 67, 4017.