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QIA39 FragEL™ DNA Fragmentation Detection Kit, Fluorescent - TdT Enzyme

MSDS (material safety data sheet) or SDS, CoA and CoQ, dossiers, brochures and other available documents.

Synonyms: TUNEL Assay

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QIA39
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Overview

Key Spec Table

Detection Methods
Fluorescence

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      Description
      OverviewAllows for detection of apoptosis at the individual cell level. Apoptosis morphology is easily detected. Detection is by flow cytometry or fluorescence microscopy. The kit mounting media permits the observance of the total cell population.
      Cat. No. QIA39 is a fluorescein-conjugated version of our widely referenced TdT Colorimetric FragEL™ DNA Fragmentation Detection Kit (Cat. No. QIA33) for non-isotopic labeling. Terminal deoxynucleotidyl transferase (TdT) binds to exposed 3′-OH ends of DNA fragments generated in response to apoptotic signals and catalyzes the addition of fluorescein-labeled and unlabeled deoxynucleotides. When excited, fluorescein generates an intense signal which can be detected by fluorescence microscopy or flow cytometry. The mounting media sustains the fluorescent signal from samples labeled on slides and, therefore, aids in the morphological evaluation and characterization of normal and apoptotic cells. Non-apoptotic cells can be visualized by a DAPI filter.
      Catalogue NumberQIA39
      Brand Family Calbiochem®
      SynonymsTUNEL Assay
      Materials Required but Not Delivered• xylene
      • ethanol, 70, 80, 90, 100%
      • 4% formaldehyde in PBS
      • 10 mM Tris pH 8
      • TBS (20 mM Tris pH 7.6, 140 mM NaCl)
      • 1 mM MgSO4 in TBS (optional, for use in generating positive control)
      • DNaseI (optional, for use in generating positive control)
      • distilled water
      • coplin jars, glass or plastic with slide holders
      • wash bottle or beaker for rinsing slides
      • humidified chamber
      • glass coverslips
      • fluorescence microscope or flow cytometer
      • 2-20 µl, 20-200 µl, and 200-1000 µl precision pipettors with disposable tips
      • microcentrifuge tubes
      • parafilm®
      • absorbent wipes
      • ice
      References
      ReferencesDarzynkiewicz, Z., et al. 1997. Cytometry 27, 1.
      Frey, T. 1997. Cytometry 28, 253.
      Shapiro, H.M. 1995. Practical Flow Cytometry, Third Edition. Wiley-Liss Inc., New York, New York.
      Darzynkiewicz, Z., et al. 1992. Cytometry 13, 795.
      Gavrieli, Y., et al. 1992. J. Cell Biol. 119, 493.
      Fawthrop, D. J., et al. 1991. Arch. Toxicol. 65, 437.
      Martin, S.J., et al. 1990. J. Immunology 145, 1859.
      Wyllie, A. H. 1980. Nature 284, 555.
      Kerr, J. F. R., et al. 1972. Br. J. Cancer 26, 239.
      Product Information
      Detection methodFluorescence
      Form50 Tests
      FormatFlow cytometry or fluorescence microscopy
      Kit containsProteinase K, Equilibration and Labeling Buffers, TdT Enzyme (Cat. No. PF060), Control Slides, Mounting Media (Cat. No. HC08), and a user protocol.
      Quality LevelMQ100
      Applications
      Key Applications Flow Cytometry
      Biological Information
      Assay time2 h
      Sample TypeFrozen or paraffin sections, fixed cell preparations or cell suspensions
      Species Reactivity
      • A Broad Range Of Species
      Safety Information
      R PhraseR: 23/25-34-36/37/38-42/43-45-61

      Toxic by inhalation and if swallowed.
      Causes burns.
      Irritating to eyes, respiratory system and skin.
      May cause sensitization by inhalation and skin contact.
      May cause cancer.
      May cause harm to the unborn child.
      S PhraseS: 26-27-36/37/39-45-53

      In case of contact with eyes, rinse immediately with plenty of water and seek medical advice.
      Take off immediately all contaminated clothing.
      Wear suitable protective clothing, gloves and eye/face protection.
      In case of accident or if you feel unwell, seek medical advice immediately (show the label where possible).
      Avoid exposure - obtain special instructions before use.
      Product Usage Statements
      Intended useThe Calbiochem® Fluorescein-FragEL™ DNA Fragmentation Detection Kit is a non-isotopic system for the labeling of DNA breaks in apoptotic nuclei in paraffin-embedded tissue sections, tissue cryosections, in cell preparations fixed on slides, or in cell suspensions. Detection and analysis of labeling is possible by flow cytometry or fluorescence microscopy.
      Storage and Shipping Information
      Ship Code Dry Ice Only
      Toxicity Multiple Toxicity Values, refer to MSDS
      Hazardous Materials Attention: Due to the nature of the Hazardous Materials in this shipment, additional shipping charges may be applied to your order. Certain sizes may be exempt from the additional hazardous materials shipping charges. Please contact your local sales office for more information regarding these charges.
      Storage -20°C
      Storage ConditionsFluorescein-FragEL™ kit components are shipped on dry ice. Upon receipt, store kit at -20°C in a non-frost-free freezer. To avoid reagent loss in tube caps, briefly pulse spin all thawed solutions before removing caps. (see General Considerations below).
      Avoid freeze/thaw Avoid freeze/thaw
      Do not freeze Ok to freeze
      Supplemental Information
      Kit containsProteinase K, Equilibration and Labeling Buffers, TdT Enzyme (Cat. No. PF060), Control Slides, Mounting Media (Cat. No. HC08), and a user protocol.
      Global Trade Item Number
      Catalogue Number GTIN
      QIA39-1EA 07790788054045