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69001 First Strand cDNA Synthesis Kit

First Strand cDNA Synthesis Kit MSDS (material safety data sheet) or SDS, CoA and CoQ, dossiers, brochures and other available documents.
69001
  
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      Overview

      Description
      Overview

      This product has been discontinued.



      The First Strand cDNA Synthesis Kit is designed for the preparation of high quality first strand cDNA from cellular RNA templates. The kit contains MMLV Reverse Transcriptase for superior yields of full-length cDNA. Both oligo(dT) and random hexamer primers are included for a choice of general priming strategies and as alternatives to user-supplied specific primers. A small volume (1-2 µl) of the first strand cDNA reaction can be used in PCR amplification with KOD Hot Start or KOD XL DNA Polymerase. Use this kit in conjunction with the Straight A’s™ mRNA Isolation System and appropriate PCR reagents to amplify rare coding regions.
      Catalogue Number69001
      Brand Family Novagen®
      Application Data
      Figure 1. The positive control RNA was subjected to first strand cDNA synthesis with various primer combinations followed by addition of the 5'-sense Control Primer (and antisense Control Primer, where appropriate) and amplification. First strand primers are indicated. AS: antisense Control Primer, RH: random hexamers, dT: oligo (dT).
      Product Information
      •4000 UMMLV Reverse Transcriptase
      •200 µl5X First Strand Buffer
      •100 µl100 mM DTT
      •50 µl10 mM dNTP Mix
      •20 µgOligo(dT) Primer
      •10 µgRandom Hexamer Primers
      •1.5 mlNuclease-free Water
      •1 µgPositive Control RNA
      •10 µlPositive Control Primers, 3' AS
      •10 µlPositive Control Primers, 5' S
      Safety Information
      R PhraseR: 22-36/38

      Harmful if swallowed.
      Irritating to eyes and skin.
      S PhraseS: 26-36

      In case of contact with eyes, rinse immediately with plenty of water and seek medical advice.
      Wear suitable protective clothing.
      Storage and Shipping Information
      Ship Code Blue Ice Only
      Toxicity Multiple Toxicity Values, refer to MSDS
      Storage Multiple storage requirements
      Avoid freeze/thaw Avoid freeze/thaw
      Do not freeze Ok to freeze
      Global Trade Item Number
      Catalogue Number GTIN
      69001 0