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344505 FTase Substrate, Fluorogenic

FTase Substrate, Fluorogenic MSDS (material safety data sheet) or SDS, CoA and CoQ, dossiers, brochures and other available documents.

Synonyms: Dansyl-GCVLS, Dns-GCVLS

344505
  
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      Overview

      Replacement Information
      Description
      Overview

      This product has been discontinued.





      This product has been discontinued.

      We apologize for the inconvenience, but we do not currently have an alternative product.





      A pentapeptide substrate based on the carboxyl terminus of H-Ras with a dansyl group attached to the amino terminus. Serves as a substrate for continuous fluorescent monitoring of protein-farnesyl transferase (FTase) activity (kcat = 0.5 s-1; Km = 1.4 µM) in the presence of farnesyl diphosphate. S-farnesylation of the cysteine moiety results in approximately 13-fold enhancement in fluorescence intensity (monitored at λ = 505 nm) accompanied by a shift in the emission maximum from 565 nm to 515 nm. Reported to be highly selective for FTase and is not recognized by geranylgeranyltransferase I. Useful for screening of potential FTase inhibitors.
      ε340 = 4,250 M-1 cm-1 in 20 mM Tris-HCI, 10 mM EDTA, pH 7.5.
      Catalogue Number344505
      Brand Family Calbiochem®
      SynonymsDansyl-GCVLS, Dns-GCVLS
      References
      ReferencesBohm, M., et al. 2001. J. Med. Chem. 44, 3117.
      Schlitzer, M., et al. 1999. Bioorg. Med. Chem. 7, 2037.
      Hightower, K.E., et al. 1998. Biochemistry 37, 15555.
      Pickett, W.C., et al. 1995. Anal. Biochem. 225, 60.
      Pompliano, D.L., et al. 1992. J. Am. Chem. Soc. 114, 7945.
      Pompliano, D.L., et al. 1992. Biochemistry 31, 3800.
      Product Information
      CAS number143744-88-1
      FormLyophilized
      FormulationSupplied as a trifluoroacetate salt.
      Hill FormulaC₃₁H₄₆N₆O₉S₂
      Chemical formulaC₃₁H₄₆N₆O₉S₂
      Hygroscopic Hygroscopic
      Structure formula ImageStructure formula Image
      Applications
      Biological Information
      Purity≥95% by HPLC
      Physicochemical Information
      Emission max.
      Excitation max.
      Dimensions
      Materials Information
      Toxicological Information
      Safety Information according to GHS
      Safety Information
      Product Usage Statements
      Storage and Shipping Information
      Ship Code Blue Ice Only
      Toxicity Standard Handling
      Storage -20°C
      Protect from Light Protect from light
      Hygroscopic Hygroscopic
      Do not freeze Ok to freeze
      Special InstructionsFollowing reconstitution aliquot and freeze (-20°C). Stock solutions are stable for up to 1 month at -20°C.
      Packaging Information
      Packaged under inert gas Packaged under inert gas
      Transport Information
      Supplemental Information
      Specifications
      Global Trade Item Number
      Catalogue Number GTIN
      344505 0

      Documentation

      FTase Substrate, Fluorogenic Certificates of Analysis

      TitleLot Number
      344505

      References

      Reference overview
      Bohm, M., et al. 2001. J. Med. Chem. 44, 3117.
      Schlitzer, M., et al. 1999. Bioorg. Med. Chem. 7, 2037.
      Hightower, K.E., et al. 1998. Biochemistry 37, 15555.
      Pickett, W.C., et al. 1995. Anal. Biochem. 225, 60.
      Pompliano, D.L., et al. 1992. J. Am. Chem. Soc. 114, 7945.
      Pompliano, D.L., et al. 1992. Biochemistry 31, 3800.
      Data Sheet

      Note that this data sheet is not lot-specific and is representative of the current specifications for this product. Please consult the vial label and the certificate of analysis for information on specific lots. Also note that shipping conditions may differ from storage conditions.

      Revision27-June-2008 RFH
      SynonymsDansyl-GCVLS, Dns-GCVLS
      DescriptionA pentapeptide substrate based on the carboxyl terminus of H-Ras with a dansyl group attached to the amino terminus. Useful for continuous fluorescent monitoring of protein-farnesyl transferase (FTase) activity in the presence of farnesyl diphosphate (kcat = 0.5 s-1; Km = 1.4 µM). Following farnesylation of the cysteine thiol the dansyl residue is placed in a lipophilic environment, resulting in an approximately 13-fold enhancement of fluorescence intensity at a wavelength of 505 nm and a shift in the emission maximum wavelength from 565 nm to 515 nm. Reported to be highly specific to FTase and is not recognized by geranylgeranyl transferase type I (GGTase I). Useful for screening potential FTase inhibitors. Excitation maximum: ~340 nm; emission maximum: ~505 nm; ε340 = 4,250 M-1cm-1 in 20 mM Tris-HCl, pH 7.5, 10 mM EDTA.
      FormLyophilized
      FormulationSupplied as a trifluoroacetate salt.
      Intert gas (Yes/No) Packaged under inert gas
      Recommended reaction conditions10 µM farnesyl diphosphate, 0.5 nM FTase, in 50 mM Tris-HCl, pH 7.5, 5 mM DTT, 5 mM MgCl2, 10 µM ZnCl2, and 0.2% octyl-β-D-glucopyranoside, at 30°C.
      CAS number143744-88-1
      Chemical formulaC₃₁H₄₆N₆O₉S₂
      Structure formulaStructure formula
      Purity≥95% by HPLC
      SolubilityDMSO (10 mg/ml)
      Storage Protect from light
      -20°C
      Hygroscopic
      Do Not Freeze Ok to freeze
      Special InstructionsFollowing reconstitution aliquot and freeze (-20°C). Stock solutions are stable for up to 1 month at -20°C.
      Toxicity Standard Handling
      ReferencesBohm, M., et al. 2001. J. Med. Chem. 44, 3117.
      Schlitzer, M., et al. 1999. Bioorg. Med. Chem. 7, 2037.
      Hightower, K.E., et al. 1998. Biochemistry 37, 15555.
      Pickett, W.C., et al. 1995. Anal. Biochem. 225, 60.
      Pompliano, D.L., et al. 1992. J. Am. Chem. Soc. 114, 7945.
      Pompliano, D.L., et al. 1992. Biochemistry 31, 3800.