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330005 EST - CAS 88321-09-9 - Calbiochem

A cell-permeable, irreversible inhibitor of cysteine proteases.

EST - CAS 88321-09-9 - Calbiochem MSDS (material safety data sheet) or SDS, CoA and CoQ, dossiers, brochures and other available documents.

Synonyms: Loxistatin, (2S,3S)-trans-Epoxysuccinyl-L-leucylamido-3-methylbutane Ethyl Ester, E-64d

Primary Target: calpain-1
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330005
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Overview

Replacement Information

Key Spec Table

CAS #Empirical Formula
88321-09-9C₁₇H₃₀N₂O₅

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      Description
      OverviewA cell-permeable, irreversible inhibitor of cysteine proteases. Similar to E-64 (Cat. No. 324890) but devoid of charged groups. Reported to inhibit calpain-1 activation. The inhibitory activity of EST has been attributed to E-64c, the free acid formed by hydrolysis of the ester in vivo. Used in animal models of muscular dystrophy.
      Catalogue Number330005
      Brand Family Calbiochem®
      SynonymsLoxistatin, (2S,3S)-trans-Epoxysuccinyl-L-leucylamido-3-methylbutane Ethyl Ester, E-64d
      References
      ReferencesInomata, M., et al. 1996. Arch. Biochem. Biophys. 328, 129.
      Mehdi, S. 1991. Trends Biochem. Sci. 16, 150.
      McGowan, E.B., et al. 1989. Biochem. Biophys. Res. Commun. 158, 432.
      Tamai, M., et al. 1987. J. Pharmacobiodyn. 10, 678.
      Komatsu, K., et al. 1986. Exp. Neurol. 91, 23.
      Tamai, M., et al. 1986. J. Pharmacobiodyn. 9, 672.
      Product Information
      CAS number88321-09-9
      ATP CompetitiveN
      FormWhite solid
      Hill FormulaC₁₇H₃₀N₂O₅
      Chemical formulaC₁₇H₃₀N₂O₅
      ReversibleN
      Structure formula ImageStructure formula Image
      Quality LevelMQ100
      Applications
      Biological Information
      Primary Targetcalpain-1
      Primary Target IC<sub>50</sub>10-100 µM for calpain-1
      Purity≥97% by HPLC
      Physicochemical Information
      Cell permeableY
      Dimensions
      Materials Information
      Toxicological Information
      Safety Information according to GHS
      RTECSRR0404300
      Safety Information
      R PhraseR: 36/37/38

      Irritating to eyes, respiratory system and skin.
      S PhraseS: 26-36

      In case of contact with eyes, rinse immediately with plenty of water and seek medical advice.
      Wear suitable protective clothing.
      Product Usage Statements
      Storage and Shipping Information
      Ship Code Ambient Temperature Only
      Toxicity Irritant
      Storage -20°C
      Do not freeze Ok to freeze
      Special InstructionsFollowing reconstitution, aliquot and freeze (-20°C). Stock solutions are stable for 6 months at -20°C.
      Packaging Information
      Transport Information
      Supplemental Information
      Specifications
      Global Trade Item Number
      Catalogue Number GTIN
      330005-1MG 04055977195699

      Documentation

      EST - CAS 88321-09-9 - Calbiochem SDS

      Title

      Safety Data Sheet (SDS) 

      EST - CAS 88321-09-9 - Calbiochem Certificates of Analysis

      TitleLot Number
      330005

      References

      Reference overview
      Inomata, M., et al. 1996. Arch. Biochem. Biophys. 328, 129.
      Mehdi, S. 1991. Trends Biochem. Sci. 16, 150.
      McGowan, E.B., et al. 1989. Biochem. Biophys. Res. Commun. 158, 432.
      Tamai, M., et al. 1987. J. Pharmacobiodyn. 10, 678.
      Komatsu, K., et al. 1986. Exp. Neurol. 91, 23.
      Tamai, M., et al. 1986. J. Pharmacobiodyn. 9, 672.

      Citations

      Title
    • Ok Hee Ryu, et al. (2005) Proteolysis of MIP-1α isoforms LD78β and LD78α by neutrophil-derived serine proteases. Journal of Biological Chemistry 280, 17415-17421.
      		•
      Data Sheet

      Note that this data sheet is not lot-specific and is representative of the current specifications for this product. Please consult the vial label and the certificate of analysis for information on specific lots. Also note that shipping conditions may differ from storage conditions.

      Revision10-July-2013 JSW
      SynonymsLoxistatin, (2S,3S)-trans-Epoxysuccinyl-L-leucylamido-3-methylbutane Ethyl Ester, E-64d
      DescriptionA cell-permeable, irreversible inhibitor of cysteine proteases. Similar to E-64 (Cat. No. 324890) but is devoid of charged groups. The inhibitory activity of EST has been attributed to E-64c, the free acid formed by hydrolysis of the ester in vivo. Used in animal models of muscular dystrophy. Recommended working concentration is 10-100 µM.
      FormWhite solid
      CAS number88321-09-9
      RTECSRR0404300
      Chemical formulaC₁₇H₃₀N₂O₅
      Structure formulaStructure formula
      Purity≥97% by HPLC
      SolubilityDMSO
      Storage -20°C
      Do Not Freeze Ok to freeze
      Special InstructionsFollowing reconstitution, aliquot and freeze (-20°C). Stock solutions are stable for 6 months at -20°C.
      Toxicity Irritant
      ReferencesInomata, M., et al. 1996. Arch. Biochem. Biophys. 328, 129.
      Mehdi, S. 1991. Trends Biochem. Sci. 16, 150.
      McGowan, E.B., et al. 1989. Biochem. Biophys. Res. Commun. 158, 432.
      Tamai, M., et al. 1987. J. Pharmacobiodyn. 10, 678.
      Komatsu, K., et al. 1986. Exp. Neurol. 91, 23.
      Tamai, M., et al. 1986. J. Pharmacobiodyn. 9, 672.
      Citation
    • Ok Hee Ryu, et al. (2005) Proteolysis of MIP-1α isoforms LD78β and LD78α by neutrophil-derived serine proteases. Journal of Biological Chemistry 280, 17415-17421.
      		•