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218696 Casein Kinase I Inhibitor, D4476 - CAS 301836-43-1 - Calbiochem

The Casein Kinase I Inhibitor, D4476, also referenced under CAS 301836-43-1, controls the biological activity of Casein Kinase I. This small molecule/inhibitor is primarily used for Phosphorylation & Dephosphorylation applications.

MSDS (material safety data sheet) or SDS, CoA and CoQ, dossiers, brochures and other available documents.

Synonyms: CK1 Inhibitor, 4-(4-(2,3-Dihydrobenzo[1,4]dioxin-6-yl)-5-pyridin-2-yl-1H-imidazol-2-yl)benzamide

Primary Target: CK1, CK1d
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218696
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Overview

Replacement Information

Key Spec Table

CAS #Empirical Formula
301836-43-1C₂₃H₁₈N₄O₃

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      Description
      OverviewA cell-permeable triaryl substituted imidazolo compound that acts as a potent, reversible and relatively specific ATP-competitive inhibitor of CK1 (IC50 = 0.2 µM from S. pombe; 0.3 µM for CK1δ) and ALK5 (IC50 = 0.5 µM). A weak inhibitor of p38α MAP kinase (IC50 = 12 µM) and only weakly affects the activities of a panel of kinases tested, including PKB, SGK and GSK-3β. Suppresses the site-specific phosphorylation (Ser322 and Ser325) and nuclear exclusion of FOXO1a in H4IIE hepatoma cells. Shown to be ~10-fold more potent than IC261 (Cat. No. 400090; IC50 = 2.5 µM for CK1).
      Catalogue Number218696
      Brand Family Calbiochem®
      SynonymsCK1 Inhibitor, 4-(4-(2,3-Dihydrobenzo[1,4]dioxin-6-yl)-5-pyridin-2-yl-1H-imidazol-2-yl)benzamide
      References
      ReferencesRena, G., et al. 2004. EMBO Reports 5, 60.
      Product Information
      CAS number301836-43-1
      ATP CompetitiveY
      FormCrystalline solid
      Hill FormulaC₂₃H₁₈N₄O₃
      Chemical formulaC₂₃H₁₈N₄O₃
      ReversibleY
      Structure formula ImageStructure formula Image
      Quality LevelMQ100
      Applications
      Biological Information
      Primary TargetCK1, CK1d
      Primary Target IC<sub>50</sub>0.2 µM against CK1 from S. pombe; 0.3 µM for CK1δ 0.5 µM against ALK5
      Purity≥95% by HPLC
      Physicochemical Information
      Cell permeableY
      Dimensions
      Materials Information
      Toxicological Information
      Safety Information according to GHS
      Safety Information
      Product Usage Statements
      Storage and Shipping Information
      Ship Code Ambient Temperature Only
      Toxicity Standard Handling
      Storage +2°C to +8°C
      Protect from Light Protect from light
      Do not freeze Ok to freeze
      Special InstructionsFollowing reconstitution, aliquot and freeze (-20°C). Stock solutions are stable for up to 6 months at -20°C.
      Packaging Information
      Packaged under inert gas Packaged under inert gas
      Transport Information
      Supplemental Information
      Specifications
      Global Trade Item Number
      Catalogue Number GTIN
      218696-1MG 04055977202496

      Documentation

      Casein Kinase I Inhibitor, D4476 - CAS 301836-43-1 - Calbiochem SDS

      Title

      Safety Data Sheet (SDS) 

      Casein Kinase I Inhibitor, D4476 - CAS 301836-43-1 - Calbiochem Certificates of Analysis

      TitleLot Number
      218696

      References

      Reference overview
      Rena, G., et al. 2004. EMBO Reports 5, 60.
      Data Sheet

      Note that this data sheet is not lot-specific and is representative of the current specifications for this product. Please consult the vial label and the certificate of analysis for information on specific lots. Also note that shipping conditions may differ from storage conditions.

      Revision30-November-2009 RFH
      SynonymsCK1 Inhibitor, 4-(4-(2,3-Dihydrobenzo[1,4]dioxin-6-yl)-5-pyridin-2-yl-1H-imidazol-2-yl)benzamide
      DescriptionA cell-permeable, reversible, potent, and relatively specific ATP-competitive inhibitor of CK1 (IC50 = 200 nM from S. pombe; 0.3 µM for CK1δ) and ALK5 (IC50 = 500 nM). A weak inhibitor of p38α MAP kinase (IC50 = 12 µM) and several other kinases tested, including PKB, SGK, and GSK-3β. Suppresses the site-specific phosphorylation (Ser322 and Ser325) and nuclear exclusion of FOXO1A in H4IIE hepatoma cells. Shown to be ~ 10-fold more potent than IC261 (Cat. No. 400090; IC50 = 2.5 µM for CK1).
      FormCrystalline solid
      Intert gas (Yes/No) Packaged under inert gas
      Recommended reaction conditionsFor use in cell culture medium dilute 1 µl of 40 mg/ml stock solution in a mixture of 6 µl serum-free medium and 3 µl GeneJuice® Transfection Reagent (Cat. No. 70967) at +21°C; add the mixture dropwise with swirling to cultured cells.
      CAS number301836-43-1
      Chemical formulaC₂₃H₁₈N₄O₃
      Structure formulaStructure formula
      Purity≥95% by HPLC
      SolubilityDMSO (40 mg/ml)
      Storage Protect from light
      +2°C to +8°C
      Do Not Freeze Ok to freeze
      Special InstructionsFollowing reconstitution, aliquot and freeze (-20°C). Stock solutions are stable for up to 6 months at -20°C.
      Toxicity Standard Handling
      ReferencesRena, G., et al. 2004. EMBO Reports 5, 60.