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CYT294 C-Reactive Protein ELISA Kit, Rat

The rat C-Reactive Protein (CRP) kit is a double polyclonal antibody sandwich enzyme immunoassay (EIA), which measures rat CRP.

C-Reactive Protein ELISA Kit, Rat MSDS (material safety data sheet) or SDS, CoA and CoQ, dossiers, brochures and other available documents.
Key Applications: ELISA Research Category: Metabolism, Cell Signaling, Epigenetics & Nuclear Function
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CYT294
1 plate  96 wells
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      Overview

      Replacement Information

      Key Spec Table

      Analytes AvailableSpecies ReactivityKey Applications
      C-Reactive Protein RELISA
      Description
      Catalogue NumberCYT294
      Brand Family Chemicon®
      Trade Name
      • Chemicon
      DescriptionC-Reactive Protein ELISA Kit, Rat
      OverviewIntroduction

      The rat C-Reactive Protein (CRP) kit is a double polyclonal antibody sandwich enzyme immunoassay (EIA), which measures rat CRP.



      Test Principle

      With the rat CRP ELISA, standards, quality controls and samples of sera are incubated in microtitration wells coated with polyclonal anti-rat CRP antibody. After a thorough wash, polyclonal anti-rat CRP antibody labelled with horseradish peroxidase (HRP) is added to the wells and incubated with the immobilized antibody-CRP complex. Following another washing step, the remaining HRP-conjugated antibody is allowed to react with the substrate and tetramethylbenzidine. The reaction is stopped by addition of acidic solution, and absorbance of the resulting yellow color product is measured spectrophotometrically at 450 nm. The absorbance is proportional to the concentration of CRP. A standard curve is constructed by plotting absorbance values versus CRP concentrations of standards, and concentrations of unknown samples are determined using this standard curve.
      Alternate Names
      • CRP
      Materials Required but Not Delivered1. Test tubes for diluting samples
      2. Precision pipettes to deliver 2--1000 mL
      3. Microplate reader with 450 ± 10 nm filter
      4. Software package facilitating data generation and analysis
      5. Microtitration plate washer (optional) [Manual washing is possible but not preferable.]
      6. Absorbent material for blotting the microwell plate
      7. Clean 250-500 mL wash bottle for buffer
      8. Adhesive cover for microplates
      9. Deionized (distilled) water
      References
      Product Information
      Components
      • Microwell Strips (96 wells), coated with Anti-rat CRP Antibody, vacuum sealed (Catalog No. CYT294a)
      • Conjugate Concentrate (100x) (Anti-rat CRP Antibody, Horseradish Peroxidase Conjugate), 0.12 mL (Catalog No. CYT294b)
      • Rat CRP Standard, 1.33 mg/mL (10x), 0.25 mL (Catalog No. CYT294c)
      • Substrate (TMB) Solution, 12 mL, Protect from light. Non-carcinogenic. (Catalog No. CYT294d)
      • Stop Solution (diluted phosphoric acid), 12 mL (Catalog No. CYT294e)
      • Wash Buffer, 1 packet (PBS with 0.05% Tween 20, pH 7.4 when reconstituted to 1 liter of distilled water) (Catalog Number CYT294f)
      HS Code3002 15 90
      Quality LevelMQ100
      Applications
      ApplicationThe rat C-Reactive Protein (CRP) kit is a double polyclonal antibody sandwich enzyme immunoassay (EIA), which measures rat CRP.
      Key Applications
      • ELISA
      Application NotesThe rat C-reactive protein (CRP) kit is designed to measure the amount of rat CRP in serum or plasma. There are enough reagents included in this kit for one 96-well immuno-assay plate. We recommend running duplicate wells for samples and standards.

      For Research Use Only; Not for use in diagnostic procedures.
      Biological Information
      Species Reactivity
      • Rat
      Analytes Available
      • C-Reactive Protein
      UniProt Number
      Physicochemical Information
      Dimensions
      Materials Information
      Toxicological Information
      Safety Information according to GHS
      Safety Information
      Product Usage Statements
      Usage Statement
      • For research use only. Not for use in diagnostic procedures.
      Storage and Shipping Information
      Storage ConditionsStore the kit at 2° to 8°C for up to 6 months after date of receipt or until expiration date.

      Precautions

      · Avoid contact with the acidic Stop Solution and Substrate Solution, which contains urea peroxide. Wear gloves and eye protection when handling these reagents. In case of contact with the Stop Solution and the Substrate Solution wash skin thoroughly with water and seek medical attention, when necessary.

      · HRP-labeled conjugate and TMB-substrate are photosensitive and are packaged in a protective opaque bottle. Store in the dark and return to storage after use.

      · Wear gloves and laboratory coats when handling the kit.

      · The materials must not be pipetted by mouth.

      · Do not drink, eat or smoke in the areas where the kit is being handled.

      · Reagents with different lot numbers should not be mixed.
      Packaging Information
      Material Size1 plate
      Material Package96 wells
      Transport Information
      Supplemental Information
      Specifications
      Global Trade Item Number
      Catalogue Number GTIN
      CYT294 04053252674648

      Documentation

      C-Reactive Protein ELISA Kit, Rat SDS

      Title

      Safety Data Sheet (SDS) 

      References

      Reference overviewPub Med ID
      Relationship between osteoinductive characteristics of biocomposite material and physicochemical characteristics of coating.
      M Z Fedorova,S V Nadezhdin,Yu R Kolobov,M B Ivanov,N A Pavlov,E V Zubareva
      Bulletin of experimental biology and medicine  148  2009

      Show Abstract       		20396802 20396802

      User Guides

      Title
      Rat C-Reactive Protein (CRP) ELISA Kit

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      Categories

      Life Science Research > Antibodies and Assays > Immunoassays > Enzyme-linked Immunosorbent Assay (ELISA) > Complete ELISA Kits
      Life Science Research > Protein Detection and Quantification > Immunoassays > Enzyme-linked Immunosorbent Assay (ELISA) > Complete ELISA Kits