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SCT275 BioTracker™ Green Late-Phase Autophagy Live Cell Probe

Fluorescence microscopy
Flow cytometry

SCT275
20 nmol  
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      Overview

      Description
      Catalogue NumberSCT275
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      DescriptionBioTracker™ Green Late-Phase Autophagy Live Cell Probe
      OverviewSCT275 is used to detect late phase autophagy in live cells. This small hydrophobic probe molecule is plasma membrane-permeable and incorporates in to the autophagosome. Following lysosome-autophagosome fusion, increased acidity in the autolysosome enhances fluorescence signal of the BioTracker™ Green Late-Phase Autophagy Live Cell Probe.

      SPECTRAL CHARACTERISTICS:
      Excitation λ: 405 nm
      Emission λ: 525 nm

      Recommended laser/filter:
      Ex 350 - 450 nm
      Em 500 nm - 560 nm
      Alternate Names
      • green autophagy probe
      • autolysosome live cell probe
      Background InformationAutophagy is an intracellular degradation process, where dysfunctional proteins and organelles are degraded by a system of organelles. Aggregated dysfunctional proteins are surrounded by the double membrane to form an autophagosome. DALGreen, which is a small hydrophobic molecule, passes the plasma membrane of live cells and is incorporated in the autophagosome.
      Product Information
      Detection methodFlow cytometry Fluorescence
      FormatLyophilized solid
      PresentationLyophilized solid.
      Quality LevelMQ100
      Applications
      ApplicationFluorescence microscopy
      Flow cytometry
      Key Applications
      • Cell Based Assays
      • Fluorescence
      • Flow Cytometry
      • Fluorescence Activated Cell Sorting (FACS)
      Application NotesSCT275 is a small hydrophobic molecule live cell probe that localizes to the autolysosome. The probe fluoresces green as the autolysosome environment acidifies, enabling the assessment of autophagy by fluorescence microscopy and flow cytometry.

      Add 20 uL DMSO to tube of SCT275 and pipet to dissolve to make a 1 mM stock solution. Dilute with culture medium to 0.1 - 1.0 uM to make working solution.
      Product Usage Statements
      Usage Statement
      • Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
      Storage and Shipping Information
      Storage ConditionsStore at -20 °C, protected from light.
      Packaging Information
      Material Size20 nmol
      Global Trade Item Number
      Catalogue Number GTIN
      SCT275 04065269218555