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SCT249 BioTracker™ Clickable Polyamine Spermidine Live Cell Probe

The BioTracker™ Clickable Polyamine Spermidine Live Cell Probe is click-conjugated to a green fluorophore to enable the study of polyamine transport with superior cellular uptake.

Probe may be dissolved in DMSO to make a 10mM stock solution.

1 uL of a 10 mM stock solution may be added to 1 mL media to produce a 10 uM working solution. Add probe/media solution to washed cells and incubate at 37C for up to 4h.

NewSCT249
500 μg  
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      Overview

      Replacement Information
      Description
      Catalogue NumberSCT249
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      DescriptionBioTracker™ Clickable Polyamine Spermidine Live Cell Probe
      OverviewThe relationship between polyamine systems and cancer---as well as critical normal cell processes--- led to the creation of clickable polyamine derivatives which are innovative and novel tools for functional studies of the polyamine transport system (PTS). Spermidine and other important polyamine derivatives were conjugated to a BODIPY fluorophore through click chemistry.

      MW: 599.44 g/mol

      SPECTRAL PROPERTIES
      λex: 494 nm
      λem: 508 nm
      Alternate Names
      • polyamine probe
      • live cell probe
      • spermidine probe
      • clickable polyamine probe
      • polyamine transport system probe
      • clickable spermidine cell probe
      Background InformationPolyamines play a variety of critical roles in the cell. Some of these processes include gene expression, cell proliferation and differentiation, and cellular stress management.

      Polyamine dysregulation is key in a variety of cancers. In colorectal cancer, polyamine content and biosynthesis is significantly higher when compared to normal colorectal tissue. A relationship between polyamines and their metabolites has been used to observe progression in cancers of the breast, lung, colorectal system, ovary, prostate, and pancreas.

      In normal cells, there is tight regulation of polyamines through normal cellular mechanisms. One of these is the polyamine transport system (PTS). The characteristics of the PTS are not fully understood, but study of the PTS has shown that many cancer cells have high PTS activity, suggesting this system as a promising target for cancer research.
      References
      Product Information
      FormatLyophilized solid
      Presentationorange-red solid
      Quality LevelMQ100
      Applications
      ApplicationThe BioTracker™ Clickable Polyamine Spermidine Live Cell Probe is click-conjugated to a green fluorophore to enable the study of polyamine transport with superior cellular uptake.

      Probe may be dissolved in DMSO to make a 10mM stock solution.

      1 uL of a 10 mM stock solution may be added to 1 mL media to produce a 10 uM working solution. Add probe/media solution to washed cells and incubate at 37C for up to 4h.
      Key Applications
      • Cell Based Assays
      • Fluorescence
      Application NotesThis spermidine derivative click-conjugated to BODIPY is optimized for the study of the polyamine transport system, which is exploited in diverse cancers.
      Biological Information
      Physicochemical Information
      Dimensions
      Materials Information
      Toxicological Information
      Safety Information according to GHS
      Safety Information
      Product Usage Statements
      Usage Statement
      • Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
      Storage and Shipping Information
      Storage ConditionsStore at -20 °C, protected from light
      Packaging Information
      Material Size500 μg
      Transport Information
      Supplemental Information
      Specifications
      Global Trade Item Number
      Catalogue Number GTIN
      SCT249 04065270804945