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07-819 Anti-phospho-EGFR (Tyr1148) Antibody

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07-819
100 µL  
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      Overview

      Key Spec Table

      Species ReactivityKey ApplicationsHostFormatAntibody Type
      HWBRbAffinity PurifiedPolyclonal Antibody
      Description
      Catalogue Number07-819
      Brand Family Upstate
      Trade Name
      • Upstate
      DescriptionAnti-phospho-EGFR (Tyr1148) Antibody
      Product Information
      FormatAffinity Purified
      Presentation100 μl of affinity purified rabbit polyclonal IgG in 50% storage buffer (PBS (without Mg2+ and Ca2+), pH 7.3 containing 1.0 mg/mL BSA (IgG, protease free) and 0.05% sodium azide) and 50% glycerol.
      Quality LevelMQ100
      Applications
      ApplicationThis Anti-phospho-EGFR (Tyr1148) Antibody is validated for use in WB for the detection of phospho-EGFR (Tyr1148).
      Key Applications
      • Western Blotting
      Application NotesImmunoblot Analysis: A 1:1000 dilution of this lot detected phospho-EGFR (Tyr1148) in RIPA lysates from EGF stimulated A431 cells.
      Biological Information
      ImmunogenPeptide corresponding to amino acid region encompassing the human, mouse, and rat phospho-EGFR (Tyr1148)
      HostRabbit
      SpecificityRecognizes phospho-EGFR (Tyr1148)
      Species Reactivity
      • Human
      Species Reactivity NotePredicted to cross-react with mouse and rat based on sequence homology (100%), but have not been tested.
      Antibody TypePolyclonal Antibody
      Entrez Gene Number
      Gene Symbol
      • EGFR
      • ERBB1
      • ERBB
      • mENA
      Modifications
      • Phosphorylation
      Purification MethodImmunoAffinity Purified
      UniProt Number
      UniProt SummaryFUNCTION: SwissProt: P00533 # Isoform 2/truncated isoform may act as an antagonist.
      SIZE: 1210 amino acids; 134277 Da
      SUBUNIT: Binds RIPK1. CBL interacts with the autophosphorylated C- terminal tail of the EGF receptor. Part of a complex with ERBB2 and either PIK3C2A or PIK3C2B. The autophosphorylated form interacts with PIK3C2B, maybe indirectly. Interacts with PELP1.
      SUBCELLULAR LOCATION: Cell membrane; Single-pass type I membrane protein. & Isoform 2: Secreted.
      TISSUE SPECIFICITY: Expressed in placenta. Isoform 2 is also expressed in ovarian cancers.
      PTM: Phosphorylation of Ser-695 is partial and occurs only if Thr- 693 is phosphorylated. & Monoubiquitinated and polyubiquitinated upon EGF stimulation; which does not affect tyrosine kinase activity or signaling capacity but may play a role in lysosomal targeting. Polyubiquitin linkage is mainly through 'Lys-63', but linkage through 'Lys-48', 'Lys-11' and 'Lys-29' also occur.DISEASE:SwissProt: P00533 # Defects in EGFR are associated with lung cancer [MIM:211980].
      SIMILARITY: SwissProt: P00533 ## Belongs to the protein kinase superfamily. Tyr protein kinase family. EGF receptor subfamily. & Contains 1 protein kinase domain.
      MISCELLANEOUS: Binding of EGF to the receptor leads to dimerization, internalization of the EGF-receptor complex, induction of the tyrosine kinase activity, stimulation of cell DNA synthesis, and cell proliferation.
      Molecular Weight185 kDa
      Product Usage Statements
      Quality AssuranceRoutinely evaluated by immunoblot.
      Usage Statement
      • Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
      Storage and Shipping Information
      Storage ConditionsStable for 2 years at -20°C from date of shipment.
      Handling Recommendations: Upon first thaw, and prior to removing the cap, centrifuge the vial and gently mix the solution. Aliquot into microcentrifuge tubes and store at -20°C. Avoid repeated freeze/thaw cycles, which may damage IgG and affect product performance. Note: Variability in freezer temperatures below -20°C may cause glycerolcontaining solutions to become frozen during storage.
      Packaging Information
      Material Size100 µL
      Global Trade Item Number
      Catalogue Number GTIN
      07-819 04053252268533