Millipore Sigma Vibrant Logo

05-740-AF488 Anti-phospho-ATM (Ser1981) Antibody, clone 10H11.E12, Alexa Fluor® 488

Anti-phospho-ATM (Ser1981) Antibody, clone 10H11.E12, Alexa Fluor® 488 is an antibody against phospho-ATM (Ser1981) for use in Immunocytochemistry.

MSDS (material safety data sheet) or SDS, CoA and CoQ, dossiers, brochures and other available documents.
Research Category: Epigenetics & Nuclear Function Research Sub-Category: Cell Cycle, DNA Replication & Repair Gene Symbol: ATM, AT UniProt Number: Q13315
View Products on Sigmaaldrich.com
05-740-AF488
100 µL  
Retrieving price...
Price could not be retrieved
Minimum Quantity is a multiple of
Maximum Quantity is
Upon Order Completion More Information
You Saved ()
 
—
Request Pricing
—
Limited Availability Limited Availability
In Stock 
Discontinued
Limited Quantities Available
Availability to be confirmed
    Remaining : Will advise
      Remaining : Will advise
      Will advise
      Contact Customer Service
      Contact Customer Service

      Special Offers

       

      Contact Customer Service

      Overview

      Replacement Information

      Special Offers

      100% Performance Guaranteed

      Key Spec Table

      Species ReactivityKey ApplicationsHostFormatAntibody Type
      H, MICCMAlexaFluor®488Monoclonal Antibody
      Description
      Catalogue Number05-740-AF488
      DescriptionAnti-phospho-ATM (Ser1981) Antibody, clone 10H11.E12, Alexa Fluor® 488
      Alternate Names
      • Serine-protein kinase ATM, Ser1981-phosphorylated
      • Ataxia telangiectasia mutated, Ser1981-phosphorylated
      • A-T mutated, Ser1981-phosphorylated
      • ATM, Ser1981-phosphorylated
      Background InformationSerine-protein kinase ATM (EC 2.7.11.1; UniProt Q13315; also known as A-T mutated, Ataxia telangiectasia mutated) is encoded by the ATM (also known as AT) gene (Gene ID 472) in human. Ataxia telangiectasia mutated kinase (ATM) and ataxia telangiectasia and Rad3-related kinase (ATR) are related kinases that regulate cell cycle checkpoints and DNA repair. Mutation in the ATM gene results in the autosomal recessive disease ataxia telangiectasia (AT). Known ATM substrates include p53, p95/NBS1, MDM2, Chk2, BRCA1, CtIP, 4E-BP1, and Chk1. The S/TQ sequence constitutes the essential substrates phosphorylation site motif. Hydrophobic amino acids at positions -3 and -1, and negatively charged amino acids at position +1 are positive determinants for substrate phosphorylation, while positively charged residues surrounding the S/TQ are negative determinants. The complex phenotypes of cells derived from patients with AT suggests that ATM has additional cellular substrates. ATM is present as an inactive homodimer or multimer prior to activation. DNA double-stranded breaks induced by ionizing radiation (IR) cause rapid ATM autophosphorylation at Ser1981 in human or the Ser1987 equivalent in mouse.
      References
      Product Information
      FormatAlexaFluor®488
      PresentationPurified mouse monoclonal IgG1κ antibody conjugate in PBS with 15 mg/mL BSA and 0.1 % sodium azide.
      Quality LevelMQ100
      Applications
      ApplicationAnti-phospho-ATM (Ser1981) Antibody, clone 10H11.E12, Alexa Fluor® 488 is an antibody against phospho-ATM (Ser1981) for use in Immunocytochemistry.
      Key Applications
      • Immunocytochemistry
      Application NotesThe unconjugated antibody (Cat. Nos. 05-740, MAB3806 & MAB3806-C) is shown to be suitable also for ELISA, Immunocytochemistry, Immunofluorescence, Immunoprecipitation, and Western blotting applications. PE-conjugate (Cat. No. FCMAB110P) is also available and tested for Flow cytometry application.
      Biological Information
      ImmunogenKLH-conjugated, synthetic peptide corresponding to amino acids 1974-1988 (SLAFEEG[pS]QSTTISS) of human ATM. The immunizing sequence is 11/12 identical to the corresponding mouse and rat sequence.
      EpitopepSer1981.
      Clone10H11.E12
      ConcentrationPlease refer to lot specific datasheet.
      HostMouse
      SpecificityReacts with ATM phosphorylated at serine 1981. Clone 10H11.E12 is covered by US patent No. 6,916,627 and 7,108,992.
      IsotypeIgG1κ
      Species Reactivity
      • Human
      • Mouse
      Antibody TypeMonoclonal Antibody
      Entrez Gene Number
      Gene Symbol
      • ATM
      • AT
      Modifications
      • Phosphorylation
      Purification MethodProtein G Purified
      UniProt Number
      Molecular WeightThe unconjugated antibody (Cat. No. 05-430) is shown to recognize the ~370 kDa phosphoATM target band and a >400 kDa non-specific band.
      Physicochemical Information
      Dimensions
      Materials Information
      Toxicological Information
      Safety Information according to GHS
      Safety Information
      Product Usage Statements
      Quality AssuranceEvaluated by Immunocytochemistry in HeLa cells.

      Immunocytochemistry Analysis: A 1:100 dilution of this antibody detected UV-induced ATM Ser1981 phosphorylation in HeLa cells.
      Usage Statement
      • Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
      Storage and Shipping Information
      Storage ConditionsStable for 1 year at 2-8°C from date of receipt.
      Packaging Information
      Material Size100 µL
      Transport Information
      Supplemental Information
      Specifications
      Global Trade Item Number
      Catalogue Number GTIN
      05-740-AF488 04055977350586

      Documentation

      Anti-phospho-ATM (Ser1981) Antibody, clone 10H11.E12, Alexa Fluor® 488 SDS

      Title

      Safety Data Sheet (SDS) 

      Anti-phospho-ATM (Ser1981) Antibody, clone 10H11.E12, Alexa Fluor® 488 Certificates of Analysis

      TitleLot Number
      Anti-phospho-ATM (Ser1981), -Q2683363 Q2683363
      Anti-phospho-ATM (Ser1981), clone 10H11.E12, Alexa Fluor® 488 - 3308163 3308163
      Anti-phospho-ATM (Ser1981), clone 10H11.E12, Alexa Fluor® 488 - 3757087 3757087
      Anti-phospho-ATM (Ser1981), clone 10H11.E12, Alexa Fluor® 488 - 4203377 4203377
      Anti-phospho-ATM (Ser1981), clone 10H11.E12, Alexa Fluor® 488 -2890748 2890748
      Anti-phospho-ATM (Ser1981), clone 10H11.E12, Alexa Fluor® 488 Monoclonal Antibody 3123163

      Related Products & Applications

      Related Products

      Catalogue Number Description  
      05-513 Anti-ATM Antibody, clone AM9 Show Pricing & Availability
      05-740 Anti-phospho-ATM (Ser1981) Antibody, clone 10H11.E12 Show Pricing & Availability
      07-1286 Anti-ATM Antibody Show Pricing & Availability
      FCMAB110P Anti-phospho-ATM (Ser1981) Antibody, clone 10H11.E12 PE conjugate Show Pricing & Availability
      MAB3806 Anti-ATM phosphoSer1981 Antibody, clone 10H11.E12 Show Pricing & Availability
      MAB3806-C Anti-phospho-ATM (Ser1981) Antibody, clone 10H11.E12, Ascites Free Show Pricing & Availability

      Categories

      Life Science Research > Antibodies and Assays > Primary Antibodies