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MABS1955-100UL Anti-mtHsp70 Antibody, clone JG1

Anti-mtHsp70, clone JG1, Cat. No. MABS1955, is a mouse monoclonal antibody that detects Heat shock 70 kDa protein 1A and has been tested for use in ELISA, Immunocytochemistry, Immunoprecipitation, and Western Blotting.

Anti-mtHsp70 Antibody, clone JG1 MSDS (material safety data sheet) or SDS, CoA and CoQ, dossiers, brochures and other available documents.
Research Category: Signaling Gene Symbol: Hspa1a, Hsp70-3, Hsp70A1 UniProt Number: Q61696
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MABS1955-100UL
100 µL  
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      Overview

      Replacement Information

      Key Spec Table

      Species ReactivityKey ApplicationsHostFormatAntibody Type
      M, HELISA, ICC, IP, WBMPurifiedMonoclonal Antibody
      Description
      Catalogue NumberMABS1955-100UL
      DescriptionAnti-mtHsp70 Antibody, clone JG1
      Alternate Names
      • Heat shock 70 kDa protein 1A
      • Heat shock 70 kDa protein 3
      • HSP70.3
      • Hsp68
      • PBP74
      Background InformationHeat shock 70 kDa protein 1A (UniProt: Q61696; also known as Heat shock 70 kDa protein 3, HSP70.3, Hsp68, PBP74, Grp75) is encoded by the Hspa1a (also known as Hsp70-3, Hsp70A1) gene (Gene ID: 193740) in murine species. The 70-kDa heat shock protein (HSP70) is highly conserved, ubiquitous protein that functions as a molecular chaperone and helps in folding of newly synthesized or denatured proteins and in activation of proteolysis of misfolded proteins and the formation and dissociation of protein complexes. Heat shock 70 kDa protein 1A is localized in cytoplasmic mRNP granules containing untranslated mRNAs. It is acetylated at Lysine 77 by NA110 in response to cellular stress and then it is
      gradually deacetylated by HDAC4 at later stages. Its acetylation enhances its chaperone activity and determines whether it will function as a chaperone for protein refolding or degradation by controlling its binding to co-chaperones HOPX and STUB1. The acetylated form and the non-acetylated form are shown to bind to HOPX and STUB1, respectively. It contains four ATP-binding regions and its N-terminal nucleotide binding domain (NBD; ATPase domain) is responsible for binding and hydrolyzing ATP. Its substrate binding domain (SBD) is localized to the C-terminal region. When ADP is bound in the NBD, a conformational change enhances the affinity of the SBD for client proteins. (Ref.: Green, JM et al. (1995). Hybridoma 14(4); 347-354).
      References
      Product Information
      FormatPurified
      PresentationPurified mouse monoclonal antibody IgG3 in PBS without azide.
      Applications
      ApplicationAnti-mtHsp70, clone JG1, Cat. No. MABS1955, is a mouse monoclonal antibody that detects Heat shock 70 kDa protein 1A and has been tested for use in ELISA, Immunocytochemistry, Immunoprecipitation, and Western Blotting.
      Key Applications
      • ELISA
      • Immunocytochemistry
      • Immunoprecipitation
      • Western Blotting
      Application NotesWestern Blotting Analysis: 1:500 dilution from a representative lot detected mtHsp70 in HeLa and U2OS cell lysates.

      Immunocytochemistry Analysis: A representative lot detected mtHsp70 in Immunocytochemistry applications (Green, J.M., et. al. (1995). Hybridoma. 14(4):347-54; McCormick, A.L., et. al. (2005). J Virol. 79(19):12205-17).

      Immunoprecipitation Analysis: A representative lot immunoprecipitated mtHsp70 in Immunoprecipitation applications (Green, J.M., et. al. (1995). Hybridoma. 14(4):347-54).

      ELISA Analysis: A representative lot detected mtHsp70 in ELISA applications (Green, J.M., et. al. (1995). Hybridoma. 14(4):347-54).

      Western Blotting Analysis: A representative lot detected mtHsp70 in Western Blotting applications (Green, J.M., et. al. (1995). Hybridoma. 14(4):347-54).
      Biological Information
      ImmunogenA linear peptide corresponding to 19 amino acids from the C-terminal region of murine mtHSP70 and conjugated to amino acids 582-599 of Tetnus toxoid at its N-terminal.
      EpitopeC-terminus
      CloneJG1
      ConcentrationPlease refer to lot specific datasheet.
      HostMouse
      SpecificityClone JG1 specifically detects Heat shock 70 kDa protein 1A (MtHSP70).
      IsotypeIgG3κ
      Species Reactivity
      • Mouse
      • Human
      Species Reactivity NoteHuman, Mouse. Predicted to react with Hamster based on 100% sequence homology.
      Antibody TypeMonoclonal Antibody
      Entrez Gene Number
      Gene Symbol
      • Hspa1a
      • Hsp70-3
      • Hsp70A1
      Purification MethodProtein G purified
      UniProt Number
      Molecular Weight~75 kDa observed; 70.08 kDa calculated. Uncharacterized bands may be observed in some lysate(s).
      Physicochemical Information
      Dimensions
      Materials Information
      Toxicological Information
      Safety Information according to GHS
      Safety Information
      Product Usage Statements
      Quality AssuranceEvaluated by Western Blotting in MCF7-10A cell lysate.

      Western Blotting Analysis: 1:500 dilution of this antibody detected mtHsp70 in MCF7-10A cell lysate.
      Usage Statement
      • Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
      Storage and Shipping Information
      Storage ConditionsStable for 1 year at -20°C from date of receipt. Handling Recommendations: Upon receipt and prior to removing the cap, centrifuge the vial and gently mix the solution. Aliquot into microcentrifuge tubes and store at -20°C. Avoid repeated freeze/thaw cycles, which may damage IgG and affect product performance.
      Packaging Information
      Material Size100 µL
      Transport Information
      Supplemental Information
      Specifications
      Global Trade Item Number
      Catalogue Number GTIN
      MABS1955-100UL 04054839519499