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ABN2247-25UG Anti-altFUS

Anti-altFUS, Cat. No. ABN2247, is a rabbit polyclonal antibody that detects altFUS and is tested for use in Immunocytochemistry and Western Blotting.

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ABN2247-25UG
25 μg  
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      Overview

      Replacement Information
      Description
      Catalogue NumberABN2247-25UG
      DescriptionAnti-altFUS
      Alternate Names
      • Alt-Fus
      Background InformationAltFus (UniProt: A0A823ADP9; also known as Alt-Fus) is encoded by the FUS gene (Gene ID: 2521) in human. FUS is a nuclear RNA-binding protein with a C-terminal nuclear localization signal. It is involved in RNA processing, DNA repair, and cell proliferation. FUS is a dual coding gene that also codes for altFUS, a highly conserved, 170 amino acid protein that is endogenously expressed in many cell lines and tissues, including the motor cortex and motor neurons. Higher expression of FUS as well as altFUS contribute to neuronal toxicity. AltFUS is embedded within the FUS gene coding sequence. It is mainly a mitochondrial protein, but a weak cytoplasmic signal is also detected. Cells overexpressing altFUS display altered mitochondrial network, with a higher number of fragmented mitochondria. It can also lead to a loss in mitochondrial potential and accumulation of cytoplasmic aggregates. AltFUS is reported to inhibit autophagy and drive the accumulation of FUS- and TDP43-positive cytoplasmic aggregates. Suppression of its expression in a Drosophila model of FUS-related toxicity is shown to protect against neurodegeneration. (Ref.: Brunet, MA., et al. (3021). EMBO Rep. 22; e50640).
      References
      Product Information
      FormatAffinity Purified
      PresentationPurified rabbit polyclonal antibody in PBS with 0.01% sodium azide.
      Quality LevelMQ200
      Applications
      ApplicationAnti-altFUS, Cat. No. ABN2247, is a rabbit polyclonal antibody that detects altFUS and is tested for use in Immunocytochemistry and Western Blotting.
      Key Applications
      • Western Blotting
      • Immunocytochemistry
      Application NotesImmunocytochemistry Analysis: A representative lot detected altFUS in Immunocytochemistry applications (Brunet, M.A., et. al. (2021). EMBO Rep. 22(1); e50640).

      Note: Actual optimal working dilutions must be determined by end user as specimens, and experimental conditions may vary with the end user
      Biological Information
      ImmunogenLinear peptides corresponding to 19 amino acids from the N-terminal region and 15 amino acids from the internal region of mitochondrial specific human altFUS.
      EpitopeInternal
      Concentration1.0 mg/mL. Please refer to guidance on suggested starting dilutions and/or titers per application and sample type.
      HostRabbit
      SpecificityThis rabbit polyclonal antibody specifically detects a mitochondrial specific 170 amino acid proteins
      IsotypeIgG
      Species Reactivity
      • Human
      Species Reactivity NoteHuman.
      Antibody TypePolyclonal Antibody
      Entrez Gene Number
      Gene Symbol
      • FUS
      Purification MethodAffinity Purified
      UniProt Number
      Molecular Weight~20 kDa observed; 17.74 kDa calculated. Uncharacterized bands may be observed in some lysate(s).
      Physicochemical Information
      Dimensions
      Materials Information
      Toxicological Information
      Safety Information according to GHS
      Safety Information
      Product Usage Statements
      Quality AssuranceEvaluated by Western Blotting in Human brain tissue lysates.

      Western Blotting Analysis: A 1:1,000 dilution of this antibody detected altFUS in Human brain tissue lysates.
      Usage Statement
      • Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
      Storage and Shipping Information
      Storage ConditionsRecommended storage: +2°C to +8°C.
      Packaging Information
      Material Size25 μg
      Transport Information
      Supplemental Information
      Specifications
      Global Trade Item Number
      Catalogue Number GTIN
      ABN2247-25UG 04065268978924