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DP02A Anti-SV40 T Antigen (Ab-2) Mouse mAb (PAb416) Agarose Conjugate

This Anti-SV40 T Antigen (Ab-2) Mouse mAb (PAb416) Agarose Conjugate is validated for use in Immunoprecipitation, Affinity Purification for the detection of SV40 T Antigen (Ab-2).

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DP02A
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Overview

Replacement Information

Key Spec Table

Species ReactivityHostAntibody Type
Sv40 Infected CellsMMonoclonal Antibody

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DP02A-0.5ML
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      Description
      OverviewRecognizes the ~94 kDa SV40 large T antigen in SV80 and SV-T2 cells. Cat. No. DP02A is linked to agarose for immunoprecipitation and purification.
      Catalogue NumberDP02A
      Brand Family Calbiochem®
      References
      ReferencesDeCaprio, J.A., et al. 1988. Cell 54, 275.
      Whyte, P., et al. 1988. Nature 334, 124.
      Mitchell, P.J., et al. 1987. Cell 50, 847.
      Dixon, R.A.F. and Nathans, D., 1985. J. Virol. 53, 1001.
      Simanis, V. and Lane, D.P., 1985. Virol. 144, 88.
      Mann, R.S. and Carroll, R.B. 1984. Virology 138, 379
      Sarnow, P., et al. 1982. Cell 28, 387.
      Crawford, L.V., et al. 1981. Proc. Natl. Acad. Sci. USA 78, 41.
      Lane, D.P. and Crawford, L.V. 1979. Nature 278, 261.
      Carroll, R.B., et al. 1974. Proc. Natl. Acad. Sci. USA 71, 3754.
      Tooze, J.1973. Cold Spring Harbor, New York.
      Tegtmeyer, P. 1972. J. Virol. 10,
      Product Information
      FormLiquid slurry
      Formulation50% slurry in PBS.
      Positive controlSV80 or SV-T2 cells
      Preservative≤0.1% sodium azide
      Quality LevelMQ100
      Applications
      Application ReferencesOriginal Clone Harlow, E., et al. 1981. J. Virol. 39, 861.
      Key Applications Affinity Purification
      Immunoprecipitation
      Application NotesImmunoprecipitation (15 µl suspension/sample)
      Affinity Purification (see comments)
      Application CommentsSuitable for affinity purification and immunoprecipitation. The amount of suspension required for purification is dependent on individual sample types.
      Biological Information
      Immunogenpurified SV40 large T-antigen
      EpitopeWithin amino acids 83-128
      ClonePAb416
      HostMouse
      IsotypeIgG2a
      Species Reactivity
      • Sv40 Infected Cells
      Antibody TypeMonoclonal Antibody
      Concentration Label Please refer to vial label for lot-specific concentration
      Physicochemical Information
      Dimensions
      Materials Information
      Toxicological Information
      Safety Information according to GHS
      Safety Information
      Product Usage Statements
      Storage and Shipping Information
      Ship Code Blue Ice Only
      Toxicity Standard Handling
      Storage +2°C to +8°C
      Do not freeze Yes
      Packaging Information
      Transport Information
      Supplemental Information
      Specifications
      Global Trade Item Number
      Catalogue Number GTIN
      DP02A-0.5ML 04055977226553

      Documentation

      Anti-SV40 T Antigen (Ab-2) Mouse mAb (PAb416) Agarose Conjugate SDS

      Title

      Safety Data Sheet (SDS) 

      Anti-SV40 T Antigen (Ab-2) Mouse mAb (PAb416) Agarose Conjugate Certificates of Analysis

      TitleLot Number
      DP02A

      References

      Reference overview
      DeCaprio, J.A., et al. 1988. Cell 54, 275.
      Whyte, P., et al. 1988. Nature 334, 124.
      Mitchell, P.J., et al. 1987. Cell 50, 847.
      Dixon, R.A.F. and Nathans, D., 1985. J. Virol. 53, 1001.
      Simanis, V. and Lane, D.P., 1985. Virol. 144, 88.
      Mann, R.S. and Carroll, R.B. 1984. Virology 138, 379
      Sarnow, P., et al. 1982. Cell 28, 387.
      Crawford, L.V., et al. 1981. Proc. Natl. Acad. Sci. USA 78, 41.
      Lane, D.P. and Crawford, L.V. 1979. Nature 278, 261.
      Carroll, R.B., et al. 1974. Proc. Natl. Acad. Sci. USA 71, 3754.
      Tooze, J.1973. Cold Spring Harbor, New York.
      Tegtmeyer, P. 1972. J. Virol. 10,
      Data Sheet

      Note that this data sheet is not lot-specific and is representative of the current specifications for this product. Please consult the vial label and the certificate of analysis for information on specific lots. Also note that shipping conditions may differ from storage conditions.

      Revision22-August-2007 RFH
      ApplicationImmunoprecipitation (15 µl suspension/sample)
      Affinity Purification (see comments)
      DescriptionPurified mouse monoclonal antibody generated by immunizing mice with the specified immunogen and fusing splenocytes with NS-1 mouse myeloma cells (see application references). Supplied as an agarose conjugate. Recognizes the ~94 kDa SV40 large T antigen.
      BackgroundSV40 T Ag (Ab-2) is a mouse monoclonal antibody with specificity for antigenic determinants unique to the SV40 large T antigen and non-reactive with SV40 small T antigen. Both antigens are encoded by the early region of the SV40 genome. The large T antigen binds DNA, and complexes with a 53,000 dalton cellular transforming protein, p53, which is required for initiation of viral DNA replication during lytic growth. In addition the large T antigen binds DNA polymerase and the transcription factor AP-2 and forms a specific complex with the p105 product of the retinoblastoma susceptibility gene.
      HostMouse
      Immunogenpurified SV40 large T-antigen
      EpitopeWithin amino acids 83-128
      ClonePAb416
      IsotypeIgG2a
      SpeciesSV40 infected cells
      Positive controlSV80 or SV-T2 cells
      FormLiquid slurry
      Formulation50% slurry in PBS.
      Concentration Label Please refer to vial label for lot-specific concentration
      Preservative≤0.1% sodium azide
      CommentsSuitable for affinity purification and immunoprecipitation. The amount of suspension required for purification is dependent on individual sample types.
      Storage +2°C to +8°C
      Do Not Freeze Yes
      Toxicity Standard Handling
      ReferencesDeCaprio, J.A., et al. 1988. Cell 54, 275.
      Whyte, P., et al. 1988. Nature 334, 124.
      Mitchell, P.J., et al. 1987. Cell 50, 847.
      Dixon, R.A.F. and Nathans, D., 1985. J. Virol. 53, 1001.
      Simanis, V. and Lane, D.P., 1985. Virol. 144, 88.
      Mann, R.S. and Carroll, R.B. 1984. Virology 138, 379
      Sarnow, P., et al. 1982. Cell 28, 387.
      Crawford, L.V., et al. 1981. Proc. Natl. Acad. Sci. USA 78, 41.
      Lane, D.P. and Crawford, L.V. 1979. Nature 278, 261.
      Carroll, R.B., et al. 1974. Proc. Natl. Acad. Sci. USA 71, 3754.
      Tooze, J.1973. Cold Spring Harbor, New York.
      Tegtmeyer, P. 1972. J. Virol. 10,
      Application referencesOriginal Clone Harlow, E., et al. 1981. J. Virol. 39, 861.

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      Categories

      Life Science Research > Antibodies and Assays > Primary Antibodies