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MABF2073 Anti-Rabies Virus CVS-11 Antibody, clone 509-6

Anti-Rabies Virus, clone 509-6, Cat. No. MABF2073, is a mouse monoclonal antibody that detects glycoprotein of Rabies virus, strain CVS-11 and has been tested for use in ELISA, Flow Cytometry, Fluorescence Activated Cell Sorting (FACS), and Neutralizing.

MSDS (material safety data sheet) or SDS, CoA and CoQ, dossiers, brochures and other available documents.
Research Category: Inflammation & Immunology Gene Symbol: G UniProt Number: O92284
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MABF2073
100 μg  
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      Overview

      Replacement Information

      Key Spec Table

      Species ReactivityKey ApplicationsHostFormatAntibody Type
      AELISA, FC, FACS, NEUTMPurifiedMonoclonal Antibody
      Description
      Catalogue NumberMABF2073
      DescriptionAnti-Rabies Virus CVS-11 Antibody, clone 509-6
      Alternate Names
      • Glycoprotein
      • Glycoprotein Rabies virus
      • Rabies virus strain CVS-11
      Background InformationGlycoprotein (UniProt: O92284) is encoded by the G gene in Rabies virus, strain CVS-11. Glycoprotein is a single-pass type I membrane protein that belongs to the lyssavirus glycoprotein family. It attached the virus to host cellular receptor, inducing endocytosis of the virion. In the endosome, the acidic pH induces conformational changes in the glycoprotein trimer, which trigger fusion between virus and cell membrane. The muscular form of the nicotinic acetylcholine receptor (nAChR), the neuronal cell adhesion molecule (NCAM), and the p75 neurotrophin receptor (p75NTR) are reported to bind glycoprotein and thereby facilitate rabies virus entry into cells. Glycoprotein structure includes a virion surface (aa 20-459), helical region (aa 460-480) and an intravirion (aa 481-524). Arginine 352 is reported to be involved in Rabies virus pathogenicity and its mutation attenuates the virus. Glycoprotein is glycosylated and palmitoylated by host and glycosylation is considered to be crucial for glycoprotein export at the cell surface. Clone 509-6 is shown to neutralize all fixed and street rabies virus strains except some virus isolates from bats. The conformational antigenic site I for this clone is located at aa 231. (Ref.: Wiktor, TJ and Koprowski, H (1980). J. Exp. Med. 152: 99-112; Marissen, WE et al (2005). J. Virol. 79(8): 4672 4678).
      References
      Product Information
      FormatPurified
      HS Code3002 15 90
      PresentationPurified mouse monoclonal antibody IgG2a in PBS without azide.
      Quality LevelMQ100
      Applications
      ApplicationAnti-Rabies Virus, clone 509-6, Cat. No. MABF2073, is a mouse monoclonal antibody that detects glycoprotein of Rabies virus, strain CVS-11 and has been tested for use in ELISA, Flow Cytometry, Fluorescence Activated Cell Sorting (FACS), and Neutralizing.
      Key Applications
      • ELISA
      • Flow Cytometry
      • Fluorescence Activated Cell Sorting (FACS)
      • Neutralizing
      Application NotesNeutralizing Analysis: A representative lot neutralized Rabies Virus (Schumacher, C.L., et. al. (1989). J Clin Invest. 84(3):971-5; Bunschoten, H., et. al. (1989). J Gen. Virol. 70 ( Pt 2):291-8; Marissen, W.E., et. al. (2005). J Virol. 79(8):4672-8).

      ELISA Analysis: A representative lot detected Rabies Virus in ELISA applications (Marissen, W.E., et. al. (2005). J Virol. 79(8):4672-8).

      Fluorescence Activated Cell Sorting (FACS) Analysis: A representative lot detected Rabies Virus in Fluorescence Activated Cell Sorting (FACS) applications (Marissen, W.E., et. al. (2005). J Virol. 79(8):4672-8).
      Biological Information
      ImmunogenBeta-propiolactone inactivated Rabies virus, strain CVS-11 (RABV).
      Clone509-6
      ConcentrationPlease refer to lot specific datasheet.
      HostMouse
      SpecificityClone 509-6 detected glycoprotein from Rabies virus, strain CVS-11 in L929 cells expressing the rabies protein. It did not detect glycoprotein in wild type L929 cells.
      IsotypeIgG2aκ
      Species Reactivity
      • All
      Species Reactivity NoteRabies Virus.
      Antibody TypeMonoclonal Antibody
      Gene Symbol
      • G
      Purification MethodProtein G purified
      UniProt Number
      Molecular Weight58.86 kDa calculated.
      Physicochemical Information
      Dimensions
      Materials Information
      Toxicological Information
      Safety Information according to GHS
      Safety Information
      Product Usage Statements
      Quality AssuranceEvaluated by Flow Cytometry in L929 cells expressing the rabies protein and in L929 (WT) cells.

      Flow Cytometry Analysis: 1 µg of this antibody detected glycoprotein of Rabies virus in one million L929 cells expressing the rabies protein.
      Usage Statement
      • Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
      Storage and Shipping Information
      Storage ConditionsStable for 1 year at -20°C from date of receipt. Handling Recommendations: Upon receipt and prior to removing the cap, centrifuge the vial and gently mix the solution. Aliquot into microcentrifuge tubes and store at -20°C. Avoid repeated freeze/thaw cycles, which may damage IgG and affect product performance.
      Packaging Information
      Material Size100 μg
      Transport Information
      Supplemental Information
      Specifications
      Global Trade Item Number
      Catalogue Number GTIN
      MABF2073 04054839367168

      Documentation

      Anti-Rabies Virus CVS-11 Antibody, clone 509-6 Certificates of Analysis

      TitleLot Number
      Anti-Rabies Virus CVS-11, clone 509-6 - 3234451 3234451
      Anti-Rabies Virus CVS-11, clone 509-6 - 3457650 3457650
      Anti-Rabies Virus CVS-11, clone 509-6 - 3804844 3804844
      Anti-Rabies Virus CVS-11, clone 509-6 - 3862808 3862808
      Anti-Rabies Virus CVS-11, clone 509-6 - 3925309 3925309
      Anti-Rabies Virus CVS-11, clone 509-6 - 4008081 4008081
      Anti-Rabies Virus CVS-11, clone 509-6 - 4109627 4109627
      Anti-Rabies Virus CVS-11, clone 509-6 Monoclonal Antibody Q2982473
      Anti-Rabies Virus CVS-11, clone 509-6 Monoclonal Antibody Q2938464