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05-488-I-100UG Anti-Pyk2/CAKβ Antibody, clone 74

Anti-Pyk2/CAKβ, clone 74, Cat. No. 05-488-I, is a mouse monoclonal antibody that detects Protein-tyrosine kinase 2b and is tested for use in Immunocytochemistry and Western Blotting.

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05-488-I-100UG
100 μg  
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      Overview

      Replacement Information
      Description
      Catalogue Number05-488-I-100UG
      DescriptionAnti-Pyk2/CAKβ Antibody, clone 74
      Alternate Names
      • Protein-tyrosine kinase 2-beta
      • EC:2.7.10.2
      • Calcium-dependent tyrosine kinase
      • CADTK
      • Calcium-regulated non-receptor proline-rich tyrosine kinase
      • Cell adhesion kinase beta
      • CAK-beta
      • CAKB
      • Focal adhesion kinase 2
      • FADK 2
      • Proline-rich tyrosine kinase 2
      Background InformationProtein-tyrosine kinase 2β (UniProt: P70600; also known as EC:2.7.10.2, Calcium-dependent tyrosine kinase, CADTK, Calcium-regulated non-receptor proline-rich tyrosine kinase, Cell adhesion kinase beta, CAK-beta, CAKB, Focal adhesion kinase 2, FADK 2, Proline-rich tyrosine kinase 2, Pyk2) is encoded by the Ptk2b (also known as Pak2, Pyk2) gene (Gene ID: 50646) in rat. Pky2 is a non-receptor protein-tyrosine kinase that regulates reorganization of the actin cytoskeleton, cell polarization, cell migration, adhesion, spreading, and bone remodeling. Its protein kinase domain is localized in amino acids 425-683. Pyk2 plays a role in the regulation of the humoral immune response and is required for normal levels of marginal B-cells in the spleen and normal migration of splenic B-cells. It is highly expressed in pulmonary vein endothelial cells, lung, and brain. It localizes to the cytoplasmic side of the membrane. Pyk2 is activated by phosphorylation of tyrosine residues in response to various stimuli that elevate intracellular calcium levels. Tyr402 is the major autophosphorylation site, but it can also be phosphorylated by other kinases. This phosphorylation promotes interaction with Src and Src family members, leading to phosphorylations at Tyr579, Tyr580, and Tyr881. Phosphorylation at Tyr402, Tyr579 and Tyr580 is required for its optimal kinase activity and phosphorylation at Tyr881 is essential for its interaction with Grb2.
      References
      Product Information
      FormatPurified
      PresentationPurified mouse monoclonal antibody IgG1 in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide.
      Quality LevelMQ200
      Applications
      ApplicationAnti-Pyk2/CAKβ, clone 74, Cat. No. 05-488-I, is a mouse monoclonal antibody that detects Protein-tyrosine kinase 2b and is tested for use in Immunocytochemistry and Western Blotting.
      Key Applications
      • Western Blotting
      • Immunocytochemistry
      Application NotesWestern Blotting Analysis: A 1:250 dilution from a representative lot detected Pyk2/CAKβ in rat brain tissue lysate.

      Western Blotting Analysis: A representative lot detected Pyk2/CAKβ in Western Blotting applications (Bernard-Trifilo, J.A., et. al. (2005). J Neurochem. 93(4):834-49).

      Immunocytochemistry Analysis: A 1:25 dilution from a representative lot detected Pyk2/CAKβ in E18 rat cortex neurons.

      Note: Actual optimal working dilutions must be determined by end user as specimens, and experimental conditions may vary with the end user
      Biological Information
      ImmunogenGST-tagged recombinant fragment corresponding to 412 amino acids from the N-terminal half of rat Protein-tyrosine kinase 2-beta (Pyk2).
      EpitopeN-terminal half
      Clone74
      Concentration1.0 mg/mL. Please refer to guidance on suggested starting dilutions and/or titers per application and sample type.
      HostMouse
      SpecificityClone 74 is a mouse monoclonal antibody that detects Protein-tyrosine kinase 2β (Pyk2). It targets an epitope within the N-terminal half.
      IsotypeIgG1
      Species Reactivity
      • Rat
      • Human
      Species Reactivity NoteHuman, Rat
      Antibody TypeMonoclonal Antibody
      Entrez Gene Number
      Gene Symbol
      • Ptk2b
      • Pak2
      • Pyk2
      Purification MethodProtein G purified
      UniProt Number
      Molecular Weight~110 kDa observed; 115.78 kDa calculated. Uncharacterized bands may be observed in some lysate(s).
      Physicochemical Information
      Dimensions
      Materials Information
      Toxicological Information
      Safety Information according to GHS
      Safety Information
      Product Usage Statements
      Quality AssuranceEvaluated by Western Blotting in Raji cell lysate.

      Western Blotting Analysis: A 1:250 dilution of this antibody detected Pyk2/CAKβ in Raji cell lysate.
      Usage Statement
      • Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
      Storage and Shipping Information
      Storage ConditionsStable for 1 year at +2°C to +8°C from date of receipt.
      Packaging Information
      Material Size100 μg
      Transport Information
      Supplemental Information
      Specifications
      Global Trade Item Number
      Catalogue Number GTIN
      05-488-I-100UG 04065265944854