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MAB4419 Anti-OCT-4 [POU5F1] Antibody, clone 7F9.2

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MAB4419
100 µg  
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      Overview

      Key Spec Table

      Species ReactivityKey ApplicationsHostFormatAntibody Type
      H, MICC, FC, WBMPurifiedMonoclonal Antibody
      Description
      Catalogue NumberMAB4419
      ReplacesMAB4305
      DescriptionAnti-OCT-4 [POU5F1] Antibody, clone 7F9.2
      Alternate Names
      • Octamer-binding transcription factor 3
      • POU class 5 homeobox 1
      • POU domain class 5, transcription factor 1
      • POU-type homeodomain-containing DNA-binding protein
      • octamer-binding transcription factor-3
      Background InformationOctamer-4 (Oct-4), a member of the POU family of transcription factors, has been demonstrated to be vital for the formation of self-renewing pluripotent stem cells. During embryogenesis, expression of Oct-4 is limited to pluripotent cells of the inner cell mass (ICM) that contribute to the formation of all fetal cell types. This relationship between Oct-4 and pluripotency has seen this transcription factor emerge as a marker of pluripotent stem cells. Undifferentiated human and murine pluripotent Embryonic Stem (ES) and Embryonic Carcinoma (EC) cells express Oct-4. Additionally, murine Embryonic Germ (EG) cells are also known to express Oct-4. Following stem cell differentiation, the level of Oct-4 expression decreases. Oct-4 expression by human EG cells is not known.
      Product Information
      FormatPurified
      PresentationPurified mouse monoclonal IgG1κ in a buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl, and 0.05% NaN3.
      Quality LevelMQ100
      Applications
      ApplicationThis Anti-OCT-4 [POU5F1] Antibody, clone 7F9.2 is validated for use in IC, FC, WB for the detection of OCT-4 [POU5F1].
      Key Applications
      • Immunocytochemistry
      • Flow Cytometry
      • Western Blotting
      Application NotesWestern Blotting: Recommended working dilution is 1 - 2 µg/mL.

      Flow Cytometry: Recommended working dilution is 2 µg/mL.

      Immunocytochemistry: Recommended working dilution is 1:100 – 1: 200.
      Biological Information
      ImmunogenRecombinant human Oct-4 protein, a.a. 1-145.
      Clone7F9.2
      ConcentrationPlease refer to the Certificate of Analysis for the lot-specific concentration.
      HostMouse
      SpecificityThis antibody reacts with the intracellular protein, Oct-4 and detects the Oct-4A isoform.
      IsotypeIgG1κ
      Species Reactivity
      • Human
      • Mouse
      Species Reactivity NoteReacts with both human and mouse Oct-4; however, reactivity to other species has not been determined.
      Antibody TypeMonoclonal Antibody
      Entrez Gene Number
      Gene Symbol
      • OCT3
      • OCT4
      • OTF3
      • OTF4
      • Oct-3
      • Oct-4
      Purification MethodProtein G sepharose column
      UniProt Number
      UniProt SummaryFUNCTION: Transcription factor that binds to the octamer motif (5'-ATTTGCAT-3'). Forms a trimeric complex with SOX2 on DNA and controls the expression of a number of genes involved in embryonic development such as YES1, FGF4, UTF1 and ZFP206. Critical for early embryogenesis and for embryonic stem cell pluripotency.
      SUBUNIT STRUCTURE:Interacts with UBE2I.
      SUBCELLULAR LOCATION: Nucleus. Note= Expressed in a diffuse and slightly punctuate pattern.
      TISSUE SPECIFICTY: Expressed the totipotent and pluripotent stem cells of the pregastrulation embryo. Also expressed in primordial germ cells and in the female germ line. Absent from adult tissues.
      DEVELOPMENTAL STAGE: Down-regulated during differentiation to endoderm and mesoderm. Ref.1
      INDUCTION: Repressed by retinoic acid (RA).
      PTM: Sumoylation enhances the protein stability, DNA binding and transactivation activity. Sumoylation is required for enhanced YES1 expression.
      SEQUENCE SIMILARITIES: Belongs to the POU transcription factor family. Class-5 subfamily.
      Contains 1 homeobox DNA-binding domain.
      Contains 1 POU-specific domain.
      Stem Cell Type
      • Human Embryonic Stem Cells
      • Mouse Embryonic Stem Cells
      • Induced Pluripotent Stem Cells
      Molecular Weight~39 kDa
      Product Usage Statements
      Usage Statement
      • Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
      Storage and Shipping Information
      Storage ConditionsMaintain refrigerated at 2-8°C in undiluted aliquots for up to 12 months.
      Packaging Information
      Material Size100 µg
      Global Trade Item Number
      Catalogue Number GTIN
      MAB4419 04053252503436