Millipore Sigma Vibrant Logo

MABN856 Anti-Neurophysin 2/NP-AVP Antibody, clone PS 45

View Products on Sigmaaldrich.com
MABN856
100 μg  
Price could not be retrieved
Minimum Quantity is a multiple of
Maximum Quantity is
Upon Order Completion More Information
You Saved ()
 
Request Pricing
Limited Availability
Limited Availability
In Stock 
Discontinued
Limited Quantities Available
Availability to be confirmed
    Remaining : Will advise
      Remaining : Will advise
      Will advise
      Contact Customer Service
      Contact Customer Service

      Special Offers

       

      Contact Customer Service

      Overview

      Key Spec Table

      Species ReactivityKey ApplicationsHostFormatAntibody Type
      R, M, B, H, FgWB, IH(P), RIA, EM, IP, ELISAMPurifiedMonoclonal Antibody
      Description
      Catalogue NumberMABN856
      DescriptionAnti-Neurophysin 2/NP-AVP Antibody, clone PS 45
      Alternate Names
      • Vasopressin-neurophysin 2-copeptin
      • AVP-NPII
      • Neurophysin 2
      • Neurophysin-II
      Background InformationThe neurophysin 1 and 2 (NPI and NPII) are carriers of peptide hormons oxytocin (OT) and vasopressin (AVP), respectively. Each NP and its associated hormon peptide are synthesized as one precursor protein, OT-NPI (UniProt P01179) encoded by the Oxt (or Ot) gene or AVP-NPII (UniProt P01186) encoded by the Avp gene in rat species. Posttranslational cleavages remove the signal peptide and process the precursor into mature OT and NPI (aa 20-28 and aa 32-135, respectively, of OT-NPI), or AVP and NPII (aa 24-32 and aa 36-128, respectively, of AVP-NPII). OT is a potent hormone and neurotransmitter secreted within the pituitary gland. OT exerts its biological activity through its receptor OXTR (also known as OTR). OT is used clinically for labor induction and may exhibit therapeutic potential for treating social anxiety disorders. AVP functions primarily as an antidiuretic and regulates the concentration of water, glucose, and salts in blood. AVP is secreted from the posterior pituitary, but exerts its biological activity in the kidney via the G protein-coupled receptor AVPR2 (V2R).
      Product Information
      FormatPurified
      PresentationPurified mouse monoclonal IgG2bκ antibody in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide.
      Quality LevelMQ100
      Applications
      ApplicationThis Anti-Neurophysin 2/NP-AVP Antibody, clone PS 45 is validated for use in Western Blotting, Immunohistochemistry (Paraffin), Radioimmunoassay, Electron Microscopy, Immunoprecipitation, ELISA for the detection of Neurophysin 2/NP-AVP.
      Key Applications
      • Western Blotting
      • Immunohistochemistry (Paraffin)
      • Radioimmunoassay
      • Electron Microscopy
      • Immunoprecipitation
      • ELISA
      Application NotesImmunohistochemistry Analysis: A 1:250-1,000 dilution from a representative lot detected Neurophysin 2/NP-AVP in rat brain and rat pituitary tissue.
      Radioimmunoassay (RIA): Target specificity of a representative lot was confirmed by RIA. Only rat neurophysin 2, but not neurophysin 1, oxytocin, or angiotensin II, can compete against 125I-neurophysin 2 for binding mAb PS 45 (Ben-Barak, Y., et al. (1985). J Neurosci. 5(1):81-97).
      Electron Microscopy (EM): A representative lot immunostained granulated neurosecretory vesicles (NSVs) in all axons, Herring bodies, and terminals by EM using rat posterior pituitary LR White sections (Ben-Barak, Y., et al. (1985). J Neurosci. 5(1):81-97).
      Immunoprecipitation Analysis: A representative lot immunoprecipitated radiolabelled neurophysin 2, but not neurophysin 1 (Ben-Barak, Y., et al. (1985). J Neurosci. 5(1):81-97).
      ELISA Analysis: A representative lot detected neurophysin 2 immunoreactivity in rat neurophysin preparations by non-sandwich ELISA using either 125I-labelled or HRP-conjugated secondary antibody (Ben-Barak, Y., et al. (1985). J Neurosci. 5(1):81-97).
      3. References/ Please add reference:
      Ben-Barak, Y., et al. (1985). J Neurosci. 5(1):81-97
      Biological Information
      ImmunogenKLH-conjugated acid soluble extract of rat (Sprague-Dawley) intermediate lobes.
      EpitopeNear Neurophysin 2 C-terminus
      ClonePS 45
      ConcentrationPlease refer to lot specific datasheet.
      HostMouse
      SpecificityClone PS 45 reacts with Vasopressin-neurophysin 2-copeptin precursor, neurophysin 2, but not Arg-vasopressin, copeptin, oxytocin, or neurophysin 1.
      IsotypeIgG2bκ
      Species Reactivity
      • Rat
      • Mouse
      • Bovine
      • Human
      • Frog
      Antibody TypeMonoclonal Antibody
      Entrez Gene Number
      Gene Symbol
      • Avp
      Purification MethodProtein G Purified
      UniProt Number
      Molecular Weight~15 kDa observed
      Product Usage Statements
      Quality AssuranceEvaluated by Western Blotting in rat pituitary tissue lysate.

      Western Blotting Analysis: 2.0 µg/mL of this antibody detected Neurophysin 2/NP-AVP in 10 µg of rat pituitary tissue lysate.
      Usage Statement
      • Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
      Storage and Shipping Information
      Storage ConditionsStable for 1 year at 2-8°C from date of receipt.
      Packaging Information
      Material Size100 μg
      Global Trade Item Number
      Catalogue Number GTIN
      MABN856 04055977277012