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MABN2620-25UG Anti-Myelin Proteolipid Protein Antibody, clone AA3

Anti-Myelin Proteolipid Protein, clone AA3, Cat. No. MABN2620, is a rat monoclonal antibody that detects Myelin proteolipid protein and is tested for use in ELISA, Immunocytochemistry, Immunohistochemistry (Paraffin), Immunoprecipitation, and Western Blot.

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MABN2620-25UG
25 μg  
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      Overview

      Replacement Information
      Description
      Catalogue NumberMABN2620-25UG
      DescriptionAnti-Myelin Proteolipid Protein Antibody, clone AA3
      Alternate Names
      • PLP
      • Lipophilin
      Background InformationMyelin proteolipid protein (UniProt: P04116; also known as PLP, DM20, Lipophilin) is encoded by the PLP1 (also known as PLP) gene (Gene ID: 281410) in bovine. Myelin proteolipid protein is a highly conserved, multi-pass membrane protein with three cytoplasmic domains, four transmembrane domains, and two extracellular domains. It is composed of a series of alternating hydrophobic and hydrophilic domains. It is the major myelin protein from the central nervous system and plays an important role in the formation or maintenance of the multilamellar structure of myelin. It is shown to be the most abundant protein of the central nervous system (CNS) myelin sheath with a relative abundance of about 17% of the myelin proteins. It is mainly expressed by myelin forming oligodendrocytes in the CNS and Schwann cells in the peripheral nervous system. PLP1 gene is reported to encode two isoforms; a 30 kDa PLP and a 25 kDa DM20 that has a deletion of 35 amino acids in the second cytoplasmic domain. The DM20 protein is expressed prior to PLP during development and as development proceeds PLP becomes the more predominant protein. Although the molecular mass of PLP is ~ 30 kDa, it migrates anomalously on SDS gels and gives a lower apparent molecular mass. (Ref.: LeVine, SM., et al. (1990). Dev. Neurosci. 12(4-5); 235-250; Yamamura, T., et al. (1991). J. Neurochem. 57(5); 1671-1680; Sarret, C., et al. (2010). Neuroscience. 166(2); 522-538).
      References
      Product Information
      FormatPurified
      PresentationPurified rat monoclonal antibody IgG2b in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide.
      Quality LevelMQ200
      Applications
      ApplicationAnti-Myelin Proteolipid Protein, clone AA3, Cat. No. MABN2620, is a rat monoclonal antibody that detects Myelin proteolipid protein and is tested for use in ELISA, Immunocytochemistry, Immunohistochemistry (Paraffin), Immunoprecipitation, and Western Blot.
      Key Applications
      • Western Blotting
      • Immunocytochemistry
      • Immunoprecipitation
      • Immunohistochemistry (Paraffin)
      • ELISA
      Application NotesWestern Blotting Analysis: A 1:1,000 dilution from a representative lot detected Myelin Proteolipid Protein in HepG2 cell lysate.

      Immunohistochemistry (Paraffin) Analysis: A 1:50 dilution from a representative lot detected Myelin Proteolipid Protein in human cerebral cortex and human cerebellum tissue sections.

      Immunoprecipitation Analysis: A representative lot immunoprecipitated Myelin Proteolipid Protein in Immunoprecipitation applications (Gudz, T.I., et. al. (2006). J Neurosci. 26(9):2458-66).

      ELISA Analysis: A representative lot detected Myelin Proteolipid Protein in ELISA applications (Yamamura, T., et. al. (1991). J Neurochem. 57(5):1671-80).

      Immunohistochemistry Applications: A representative lot detected Myelin Proteolipid Protein in Immunohistochemistry applications ( Gudz, T.I., et. al. (2006). J Neurosci. 26(9):2458-66; Sarret, C., et. al. (2010). Neuroscience. 166(2):522-38).

      Western Blotting Analysis: A representative lot detected Myelin Proteolipid Protein in Western Blotting applications (Zhu, H., et. al. (2012). Glia. 60(1):69-82).

      Immunocytochemistry Analysis: A representative lot detected Myelin Proteolipid Protein in Immunocytochemistry applications (Sarret, C., et. al. (2010). Neuroscience. 166(2):522-38).

      Note: Actual optimal working dilutions must be determined by end user as specimens, and experimental conditions may vary with the end user
      Biological Information
      ImmunogenPurified Myelin proteolipid protein from bovine brain.
      EpitopeC-terminal
      CloneAA3
      Concentration1.0 mg/mL. Please refer to guidance on suggested starting dilutions and/or titers per application and sample type.
      HostRat
      SpecificityClone AA3 is a rat monoclonal antibody that detects Myelin proteolipid protein. It targets an epitope within 13 amino acids from the C-terminal region. It detects both isoforms of Myelin proteolipid protein.
      IsotypeIgG2bκ
      Species Reactivity
      • Rat
      • Mouse
      • Human
      Species Reactivity NoteHuman, Mouse, Rat. Predicted to react with Bovine based on 100% sequence homology.
      Antibody TypeMonoclonal Antibody
      Entrez Gene Number
      Gene Symbol
      • PLP1
      • PLP
      Purification MethodProtein G purified
      UniProt Number
      Molecular Weight~17 kDa observed; 30.02 kDa calculated. Uncharacterized bands may be observed in some lysate(s).
      Physicochemical Information
      Dimensions
      Materials Information
      Toxicological Information
      Safety Information according to GHS
      Safety Information
      Product Usage Statements
      Quality AssuranceEvaluated by Western Blotting in SH-SY5Y cell lysate.

      Western Blotting Analysis: A 1:1,000 dilution of this antibody detected Myelin Proteolipid Protein in SH-SY5Y cell lysate.
      Usage Statement
      • Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
      Storage and Shipping Information
      Storage ConditionsStable for 1 year at +2°C to +8°C from date of receipt.
      Packaging Information
      Material Size25 μg
      Transport Information
      Supplemental Information
      Specifications
      Global Trade Item Number
      Catalogue Number GTIN
      MABN2620-25UG 04065266020755

      Documentation

      Anti-Myelin Proteolipid Protein Antibody, clone AA3 SDS

      Title

      Safety Data Sheet (SDS) 

      Anti-Myelin Proteolipid Protein Antibody, clone AA3 Certificates of Analysis

      TitleLot Number
      Anti-Myelin Proteolipid Protein, clone AA3 - 4023763 4023763
      Anti-Myelin Proteolipid Protein, clone AA3 - 4025561 4025561
      Anti-Myelin Proteolipid Protein, clone AA3 - 4028623 4028623
      Anti-Myelin Proteolipid Protein, clone AA3 - 4033104 4033104
      Anti-Myelin Proteolipid Protein, clone AA3 - 4034325 4034325
      Anti-Myelin Proteolipid Protein, clone AA3 - 4038978 4038978
      Anti-Myelin Proteolipid Protein, clone AA3 - 4042599 4042599
      Anti-Myelin Proteolipid Protein, clone AA3 - 4044764 4044764
      Anti-Myelin Proteolipid Protein, clone AA3 - 4074328 4074328
      Anti-Myelin Proteolipid Protein, clone AA3 - 4137663 4137663
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