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IM72 Anti-MMP-9 (Ab-8) Mouse mAb (IA5)

Anti-MMP-9 (Ab-8) Mouse mAb (IA5) MSDS (material safety data sheet) or SDS, CoA and CoQ, dossiers, brochures and other available documents.

Synonyms: Anti-Gelatinase B, Anti-92 kDa Gelatinase, Anti-Matrix Metalloproteinase 9, Anti-Type IV Collagenase

IM72
  
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      Overview

      Replacement Information

      Key Spec Table

      Host
      M
      Description
      Overview

      This product has been discontinued.





      Recognizes the ~92 kDa latent and the ~83 kDa active forms of MMP-9 in HUVEC and dexamethasone-treated HT1080 cells.
      Catalogue NumberIM72
      Brand Family Calbiochem®
      SynonymsAnti-Gelatinase B, Anti-92 kDa Gelatinase, Anti-Matrix Metalloproteinase 9, Anti-Type IV Collagenase
      References
      ReferencesCottam, D.W. and Rees, R.C. 1993. Intl. J. Oncol. 2, 861.
      Stetler-Stevenson, W.G., et al. 1993. FASEB J. 7, 1434.
      Woessner, J.F. 1991. FASEB J. 5, 2145.
      Liotta, L.A. and Stetler-Stevenson, W.G. 1990. in Seminars in Cancer Biology, ed. M.M. Gottesman. Vol. 1, 99.
      Product Information
      DeclarationNot available for sale in Japan.
      FormLiquid
      FormulationIn 10 mM PBS, 0.2% BSA, pH 7.4.
      Positive controlConditioned medium from dexamethasone-treated HT-1080 or HUVEC cells
      Preservative≤0.1% sodium azide
      Applications
      Key Applications Immunoblotting (Western Blotting)
      Immunofluorescence
      Not Frozen Sections
      Not Paraffin Sections
      Application NotesFrozen Sections (not recommended)
      Immunoblotting (2-4 µg/ml)
      Immunofluorescence (see comments)
      Paraffin Sections (not recommended)
      Application CommentsThis antibody has also been shown to work for immunofluorescence. Antibody should be titrated for optimal results in individual systems.
      Biological Information
      Immunogennative, human pro-MMP-9
      ImmunogenHuman
      CloneIA5
      HostMouse
      IsotypeIgG₁
      Concentration Label Please refer to vial label for lot-specific concentration
      Physicochemical Information
      Dimensions
      Materials Information
      Toxicological Information
      Safety Information according to GHS
      Safety Information
      Product Usage Statements
      Storage and Shipping Information
      Ship Code Blue Ice Only
      Toxicity Standard Handling
      Storage +2°C to +8°C
      Do not freeze Ok to freeze
      Special InstructionsFor long-term storage, aliquot and freeze (-20°C). Avoid freeze/thaw cycles.
      Packaging Information
      Transport Information
      Supplemental Information
      Specifications
      Global Trade Item Number
      Catalogue Number GTIN
      IM72 0

      Documentation

      Anti-MMP-9 (Ab-8) Mouse mAb (IA5) SDS

      Title

      Safety Data Sheet (SDS) 

      Anti-MMP-9 (Ab-8) Mouse mAb (IA5) Certificates of Analysis

      TitleLot Number
      IM72

      References

      Reference overview
      Cottam, D.W. and Rees, R.C. 1993. Intl. J. Oncol. 2, 861.
      Stetler-Stevenson, W.G., et al. 1993. FASEB J. 7, 1434.
      Woessner, J.F. 1991. FASEB J. 5, 2145.
      Liotta, L.A. and Stetler-Stevenson, W.G. 1990. in Seminars in Cancer Biology, ed. M.M. Gottesman. Vol. 1, 99.
      Data Sheet

      Note that this data sheet is not lot-specific and is representative of the current specifications for this product. Please consult the vial label and the certificate of analysis for information on specific lots. Also note that shipping conditions may differ from storage conditions.

      Revision04-September-2007 RFH
      SynonymsAnti-Gelatinase B, Anti-92 kDa Gelatinase, Anti-Matrix Metalloproteinase 9, Anti-Type IV Collagenase
      ApplicationFrozen Sections (not recommended)
      Immunoblotting (2-4 µg/ml)
      Immunofluorescence (see comments)
      Paraffin Sections (not recommended)
      DescriptionPurified mouse monoclonal antibody generated by immunizing BALB/c mice with the specified immunogen and fusing splenocytes with p3-X63-Ag8.653 mouse myeloma cells. Recognizes the ~92 kDa latent and the ~86 kDa active forms of MMP-9.
      BackgroundMatrix metalloproteinases (MMPs) are a family of enzymes that are responsible for the degradation of extracellular matrix components such as collagen, laminin and proteoglycans. In addition to sequence homology, all MMPs share the following characteristics: the catalytic mechanism is dependent upon a zinc ion at the active center, they cleave one or more extracellular matrix components, they are secreted as zymogens which are activated by removal of an ~10 kDa segment from the N-terminus and they are inhibited by tissue inhibitor of metalloproteinases (TIMP). These enzymes are involved in normal physiological processes such as embryogenesis and tissue remodeling and may play an important role in angiogenesis, arthritis, periodontitis, and metastasis. Matrix metalloproteinase-9 (MMP-9) also known as gelatinase B, 92 kDa gelatinase/type IV collagenase (EC 3.4.24.35) has a broad range of substrate specificity for native collagens including types IV, V, VII, and X as well as gelatin, proteoglycans and elastin. MMP-9 is secreted as a 92 kDa proenzyme and can be activated in vitro by organomercurials (e.g., 4-aminophenylmercuric acetate, APMA), trypsin, and α-chymotrypsin and in vivo by cathepsin G and MMP-3 to the 86 kDa active form. The activity of MMP-9 can be inhibited by TIMPs in a 1:1 molar ratio. TIMP-1 is known to bind exclusively to pro-MMP-9 (Kd ~35 nM) whereas TIMP-2 binds to pro-MMP-2 with a Kd ~5 nM. MMP-9 has been measured in human plasma from normal individuals and has been shown to be elevated in sera of patients with hepatocellular carcinomas.
      HostMouse
      Immunogen speciesHuman
      Immunogennative, human pro-MMP-9
      CloneIA5
      IsotypeIgG₁
      Specieshuman
      Positive controlConditioned medium from dexamethasone-treated HT-1080 or HUVEC cells
      FormLiquid
      FormulationIn 10 mM PBS, 0.2% BSA, pH 7.4.
      Concentration Label Please refer to vial label for lot-specific concentration
      Preservative≤0.1% sodium azide
      CommentsThis antibody has also been shown to work for immunofluorescence. Antibody should be titrated for optimal results in individual systems.
      Storage +2°C to +8°C
      Do Not Freeze Ok to freeze
      Special InstructionsFor long-term storage, aliquot and freeze (-20°C). Avoid freeze/thaw cycles.
      Toxicity Standard Handling
      ReferencesCottam, D.W. and Rees, R.C. 1993. Intl. J. Oncol. 2, 861.
      Stetler-Stevenson, W.G., et al. 1993. FASEB J. 7, 1434.
      Woessner, J.F. 1991. FASEB J. 5, 2145.
      Liotta, L.A. and Stetler-Stevenson, W.G. 1990. in Seminars in Cancer Biology, ed. M.M. Gottesman. Vol. 1, 99.