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MABF2678-100UL Anti-KSHV gpK8.1A Antibody, clone 4A4

Anti-KSHV gpK8.1A, clone 4A4, Cat. No. MABF2678, is a mouse monoclonal antibody that detects Kaposi's sarcoma-associated herpesvirus (KSHV) and is tested in Electron Microscopy, ELISA, Immunocytochemistry, Inhibition Assay, and Western Blotting.

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MABF2678-100UL
100 µL  
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      Overview

      Replacement Information
      Description
      Catalogue NumberMABF2678-100UL
      DescriptionAnti-KSHV gpK8.1A Antibody, clone 4A4
      Alternate Names
      • Kaposi's sarcoma-associated herpesvirus glycoprotein K8.1
      • Glycoprotein K8.1
      • Glycoprotein K8.1A
      • Glycoprotein gp35/37
      • K8.1
      • K8.1 protein
      Background InformationKaposi's sarcoma-associated herpesvirus glycoprotein K8.1 (UniProt: O36551; also known as Glycoprotein K8.1, Glycoprotein K8.1A, Glycoprotein gp35/37, K8.1, K8.1 protein) is encoded by the K8.1 (also known as HHV8GK18_gp55) gene (Gene ID: 4961469) in Kaposi's sarcoma-associated herpesvirus (KSHV). Kaposi sarcoma virus is an oncogenic human g-herpesvirus that has been identified in AIDS patients in association with Kaposi's sarcoma. It can persist latently in host cells while expressing only a limited set of viral genes and maintaining its genome as extrachromosomal plasmids. This virus encodes two immunogenic class I glycoproteins, K8.1A and K8.1B. These glycoproteins have several serine and threonine residues that serves as potential sites for O-glycosylation. The 228-amino-acid-long K8.1A open reading frame (ORF) contains four N-glycosylation sites and the 167-amino-acid-long K8.1B ORF contains three N-glycosylation sites. Glycoprotein K8.1A is reported to mediate KSHV binding to target cells via interaction with heparan sulfate. This protein is involved in binding to target cells but is not required for viral entry into cells. Clone 4A4 detects both K8.1A and K8.1B glycoprotein and is shown to react with infected cell membranes and virion envelope. This antibody is shown to partially inhibit KSHV infection in HEK293 cells. (Ref.: Faure, A., et al. (2019). Proc. Natl. Acad. Sci. USA. 116(33); 16519-16528; Zhu, L., et al. (1999). Virology 262(1), 237-249).
      References
      Product Information
      FormatPurified
      PresentationPurified mouse monoclonal antibody IgG1 in PBS without azide.
      Quality LevelMQ200
      Applications
      ApplicationAnti-KSHV gpK8.1A, clone 4A4, Cat. No. MABF2678, is a mouse monoclonal antibody that detects Kaposi's sarcoma-associated herpesvirus (KSHV) and is tested in Electron Microscopy, ELISA, Immunocytochemistry, Inhibition Assay, and Western Blotting.
      Key Applications
      • Western Blotting
      • Inhibition
      • Electron Microscopy
      • ELISA
      • Immunocytochemistry
      Application NotesTested Applications

      Inhibition: A representative lot of this antibody inhibited infection by KSHV in HEK293 cells.(Faure, A., et al. (2019). Proc Natl Acad Sci USA. 116(33):16519-16528).

      Electron Microscopy: A representative lot of this antibody detected K8.1A glycoprotein in TPA stimulated BCBL-1 cells. (Zhu, L., et al. (1999). Virology. 262(1):237-49).

      Western Blotting Analysis: A representative lot detected KSHV gpK8.1A in Western Blotting applications (Zhu, L., et al. (1999). Virology. 262(1):237-49).

      ELISA Analysis: A representative lot detected KSHV gpK8.1A in ELISA applications (Zhu, L., et al. (1999). Virology. 262(1):237-49).

      Immunocytochemistry Analysis: A representative lot detected KSHV gpK8.1A in Immunocytochemistry applications (Zhu, L., et al. (1999). Virology. 262(1):237-49).

      Note: Actual optimal working dilutions must be determined by end user as specimens, and experimental conditions may vary with the end user
      Biological Information
      ImmunogenPhorbol-12-myristate-13-acetate TPA))- induced BCBL-1 (Body Cavity Based Lymphoma-1) cells.
      EpitopeUnknown
      Clone4A4
      Concentration0.5 mg/mL. Please refer to guidance on suggested starting dilutions and/or titers per application and sample type.
      HostMouse
      SpecificityClone 4A4 is a mouse monoclonal antibody that detects Glycoprotein K8.1 of Kaposi's sarcoma-associated herpesvirus.
      IsotypeIgG1κ
      Species Reactivity
      • Virus
      Species Reactivity NoteKaposi's sarcoma-associated herpesvirus
      Antibody TypeMonoclonal Antibody
      Entrez Gene Number
      Gene Symbol
      • K8.1
      Purification MethodProtein G purified
      UniProt Number
      Molecular Weight~35 kDa observed; 24.81 kDa calculated. Uncharacterized bands may be observed in some lysate(s).
      Physicochemical Information
      Dimensions
      Materials Information
      Toxicological Information
      Safety Information according to GHS
      Safety Information
      Product Usage Statements
      Quality AssuranceEvaluated by Western Blotting in Phorbol-12-myristate-13-acetate (TPA)-induced BCBL-1 cells.

      Western Blotting Analysis (WB): A 1:1,000 dilution of this antibody detected Kaposi's sarcoma-associated herpesvirus (KSHV) in Phorbol-12-myristate-13-acetate (TPA)- induced BCBL-1 cells.
      Usage Statement
      • Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
      Storage and Shipping Information
      Storage ConditionsStore at -10°C to -25°C. Handling Recommendations: Upon receipt and prior to removing the cap, centrifuge the vial and gently mix the solution. Aliquot into microcentrifuge tubes and store at -20°C. Avoid repeated freeze/thaw cycles, which may damage IgG and affect product performance.
      Packaging Information
      Material Size100 µL
      Transport Information
      Supplemental Information
      Specifications
      Global Trade Item Number
      Catalogue Number GTIN
      MABF2678-100UL 04065267780696