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GR07 Anti-Insulin Receptor (Ab-3) Mouse mAb (29B4)

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GR07
  
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      Overview

      Replacement Information

      Key Spec Table

      Species ReactivityHostAntibody Type
      H, M, RMMonoclonal Antibody
      Description
      Overview

      This product has been discontinued.



      Recognizes the ~95 kDa β chain of the insulin receptor protein. Cross-reacts with IGF-I receptor.
      Catalogue NumberGR07
      Brand Family Calbiochem®
      References
      ReferencesGrigorescu, F., et al. 1987. J. Clin. Endocrinol, Metab. 64, 549.
      Hari, J. and Roth, R.A. 1987. J. Biol. Chem. 262, 15341.
      Rosen, O.M. 1987. Science 237, 1452.
      Morgan, D.O., et al. 1986. Proc. Natl. Acad., Sci. USA 83, 328.
      White, M.F. and Kahn, C.R., 1986. The Enzymes 17, 247.
      Ganguly, S., et al. 1985. Academic Press, Inc. 27, 83.
      Grunberger, G., et al. 1984. Science 223, 932.
      Product Information
      FormLiquid
      FormulationIn 50 mM sodium phosphate buffer, 0.2% gelatin.
      Negative controlNormal skin fibroblasts
      Positive controlHepG2 cells
      Preservative≤0.1% sodium azide
      Quality LevelMQ100
      Applications
      Application ReferencesOriginal Clone, Neutralization Studies Morgan, D.O. and Roth, R.A. 1986. Biochemistry 25, 1364.
      Key Applications Immunoprecipitation
      Not Western Blot
      Not Paraffin Sections
      Neutralization Studies
      Application NotesImmunoblotting (not recommended)
      Immunoprecipitation (1-2 µg/sample)
      Neutralization Studies (see comments and application references)
      Paraffin Sections (not recommended)
      Application CommentsThe antibody has been reported to produce partial inhibition of insulin-dependent protein-tyrosine kinase activity (see application references). Also reported to cross-react with IGF-I receptor. Antibody should be titrated for optimal results in individual systems.
      Biological Information
      Immunogenpartially purified, human, placental insulin receptor
      ImmunogenHuman
      Clone29B4
      HostMouse
      IsotypeIgG₁
      Species Reactivity
      • Human
      • Mouse
      • Rat
      Antibody TypeMonoclonal Antibody
      Concentration Label Please refer to vial label for lot-specific concentration
      Physicochemical Information
      Dimensions
      Materials Information
      Toxicological Information
      Safety Information according to GHS
      Safety Information
      Product Usage Statements
      Storage and Shipping Information
      Ship Code Blue Ice Only
      Toxicity Standard Handling
      Storage +2°C to +8°C
      Do not freeze Yes
      Packaging Information
      Transport Information
      Supplemental Information
      Specifications
      Global Trade Item Number
      Catalogue Number GTIN
      GR07 0

      Documentation

      Anti-Insulin Receptor (Ab-3) Mouse mAb (29B4) Certificates of Analysis

      TitleLot Number
      GR07

      References

      Reference overview
      Grigorescu, F., et al. 1987. J. Clin. Endocrinol, Metab. 64, 549.
      Hari, J. and Roth, R.A. 1987. J. Biol. Chem. 262, 15341.
      Rosen, O.M. 1987. Science 237, 1452.
      Morgan, D.O., et al. 1986. Proc. Natl. Acad., Sci. USA 83, 328.
      White, M.F. and Kahn, C.R., 1986. The Enzymes 17, 247.
      Ganguly, S., et al. 1985. Academic Press, Inc. 27, 83.
      Grunberger, G., et al. 1984. Science 223, 932.
      Data Sheet

      Note that this data sheet is not lot-specific and is representative of the current specifications for this product. Please consult the vial label and the certificate of analysis for information on specific lots. Also note that shipping conditions may differ from storage conditions.

      Revision24-August-2007 RFH
      ApplicationImmunoblotting (not recommended)
      Immunoprecipitation (1-2 µg/sample)
      Neutralization Studies (see comments and application references)
      Paraffin Sections (not recommended)
      DescriptionPurified mouse monoclonal antibody generated by immunizing SJL mice with the specified immunogen and fusing splenocytes with Sp2/0 mouse myeloma cells (see application references). Recognizes the ~95 kDa β chain of insulin receptor.
      BackgroundInsulin initiates its effects on cells through interaction with high-affinity, cell surface, glycoprotein receptors, consisting of two α (135,000 dalton) and two β (95,000 dalton) subunits linked by disulfide bonds. The α subunits bind insulin and the β subunits become phosphorylated in response to insulin. Insulin-dependent phosphorylation occurs at multiple sites near the carboxyl terminus and in the kinase domain. The receptor protein-tyrosine kinase has been implicated as the mediator of most, if not all, of the effects of insulin. Mutations that diminish both phosphorylation and internalization of the insulin receptor block insulin action, as do antibodies which inhibit its protein-tyrosine kinase activity. Cells from patients with diabetic syndromes, characterized by nonresponsiveness to insulin, exhibit reduced insulin dependent protein-tyrosine kinase activity.
      HostMouse
      Immunogen speciesHuman
      Immunogenpartially purified, human, placental insulin receptor
      Clone29B4
      IsotypeIgG₁
      Specieshuman, mouse, rat
      Positive controlHepG2 cells
      Negative controlNormal skin fibroblasts
      FormLiquid
      FormulationIn 50 mM sodium phosphate buffer, 0.2% gelatin.
      Concentration Label Please refer to vial label for lot-specific concentration
      Preservative≤0.1% sodium azide
      CommentsThe antibody has been reported to produce partial inhibition of insulin-dependent protein-tyrosine kinase activity (see application references). Also reported to cross-react with IGF-I receptor. Antibody should be titrated for optimal results in individual systems.
      Storage +2°C to +8°C
      Do Not Freeze Yes
      Toxicity Standard Handling
      ReferencesGrigorescu, F., et al. 1987. J. Clin. Endocrinol, Metab. 64, 549.
      Hari, J. and Roth, R.A. 1987. J. Biol. Chem. 262, 15341.
      Rosen, O.M. 1987. Science 237, 1452.
      Morgan, D.O., et al. 1986. Proc. Natl. Acad., Sci. USA 83, 328.
      White, M.F. and Kahn, C.R., 1986. The Enzymes 17, 247.
      Ganguly, S., et al. 1985. Academic Press, Inc. 27, 83.
      Grunberger, G., et al. 1984. Science 223, 932.
      Application referencesOriginal Clone, Neutralization Studies Morgan, D.O. and Roth, R.A. 1986. Biochemistry 25, 1364.