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AP1153 Anti-IL12RB2 Mouse mAb (2H6)

AP1153
  
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      Overview

      Replacement Information

      Key Spec Table

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      Description
      Overview

      This product has been discontinued.





      Recognizes the IL12RB2 protein.
      Catalogue NumberAP1153
      Brand Family Calbiochem®
      SynonymsAnti-Interleukin 12 Receptor β2
      Application Data
      Detection of human IL12RB2 by immunoblotting. Sample: Partial recombinant IL12RB2 human protein. Primary antibody: Anti-IL12RB2 Mouse mAb (2H6) (Cat. No. AP1153) (1-5 µg/ml). Detection: chemiluminescence.
      References
      Product Information
      FormLiquid
      FormulationIn PBS, pH 7.2.
      PreservativeNone
      Applications
      Key Applications Enzyme-Linked Immunosorbent Assay
      Immunoblotting (Western Blotting)
      Application NotesELISA (see comments)
      Immunoblotting (1-5 µg/ml)
      Application CommentsFor ELISA, this antibody can be used as the capture antibody; recommended concentration is 10 ng/ml, 1 µg/well, 100 µl total volume. Variables associated with assay conditions will dictate the proper working dilution.
      Biological Information
      ImmunogenA recombinant polypeptide corresponding to amino acids 105-215 of human IL12RB2, expressed as a GST fusion protein
      Clone2H6
      HostMouse
      IsotypeIgG2a
      Physicochemical Information
      Dimensions
      Materials Information
      Toxicological Information
      Safety Information according to GHS
      Safety Information
      Product Usage Statements
      Storage and Shipping Information
      Ship Code Blue Ice Only
      Toxicity Regulatory Review
      Storage -20°C
      Avoid freeze/thaw Avoid freeze/thaw
      Do not freeze Ok to freeze
      Special InstructionsFollowing initial thaw, aliquot and freeze (-20°C).
      Packaging Information
      Transport Information
      Supplemental Information
      Specifications
      Global Trade Item Number
      Catalogue Number GTIN
      AP1153 0

      Documentation

      Data Sheet

      Note that this data sheet is not lot-specific and is representative of the current specifications for this product. Please consult the vial label and the certificate of analysis for information on specific lots. Also note that shipping conditions may differ from storage conditions.

      Revision27-October-2010 JLN
      SynonymsAnti-Interleukin 12 Receptor β2
      ApplicationELISA (see comments)
      Immunoblotting (1-5 µg/ml)
      Application Data
      Detection of human IL12RB2 by immunoblotting. Sample: Partial recombinant IL12RB2 human protein. Primary antibody: Anti-IL12RB2 Mouse mAb (2H6) (Cat. No. AP1153) (1-5 µg/ml). Detection: chemiluminescence.
      DescriptionPurified mouse monoclonal antibody. Recognizes the IL12RB2 protein.
      BackgroundIL12RB2 is a type I transmembrane protein identified as a subunit of the interleukin 12 receptor complex. The coexpression of IL12RB2 and IL12RB1 proteins is shown to lead to the formation of high-affinity IL12 binding sites and the reconstitution of IL12 dependent signaling. The expression of this gene is up-regulated by interferon gamma in Th1 cells, and plays a role in Th1 cell differentiation. The up-regulation of this gene is found to be associated with a number of infectious diseases, such as Crohn's disease and leprosy, which is thought to contribute to the inflammatory response and host defense.
      HostMouse
      ImmunogenA recombinant polypeptide corresponding to amino acids 105-215 of human IL12RB2, expressed as a GST fusion protein
      Clone2H6
      IsotypeIgG2a
      Specieshuman
      FormLiquid
      FormulationIn PBS, pH 7.2.
      PreservativeNone
      CommentsFor ELISA, this antibody can be used as the capture antibody; recommended concentration is 10 ng/ml, 1 µg/well, 100 µl total volume. Variables associated with assay conditions will dictate the proper working dilution.
      Storage Avoid freeze/thaw
      -20°C
      Do Not Freeze Ok to freeze
      Special InstructionsFollowing initial thaw, aliquot and freeze (-20°C).
      Toxicity Regulatory Review