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MAB1285 Anti-HLA-B27 Antibody, clone HLA.ABC.m3

This Anti-HLA-B27 Antibody, clone HLA.ABC.m3 is validated for use in FC, IC for the detection of HLA-B27.

Anti-HLA-B27 Antibody, clone HLA.ABC.m3 MSDS (material safety data sheet) or SDS, CoA and CoQ, dossiers, brochures and other available documents.
Research Category: Inflammation & Immunology Research Sub-Category: Immunoglobulins & Immunology Gene Symbol: HLA-B, Bw-54, Bw-45, Bw-47, Bw-46, Bw-52, B-5, Bw-4, Bw-57, OTTHUMP00000034826, AS, Bw-56, Bw-55, Bw-41, Bw-48, B'DT, Bw-50, Bw-60, Bw-58, B-21, HLA-B-5501, B-12, Bw-22, Bw-53, HLAB, SPDA1, Bw-44 UniProt Number: P03989
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MAB1285
100 µg  
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      Overview

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      Key Spec Table

      Species ReactivityKey ApplicationsHostFormatAntibody Type
      HFC, ICCMPurifiedMonoclonal Antibody
      Description
      Catalogue NumberMAB1285
      Brand Family Chemicon®
      Trade Name
      • Chemicon
      DescriptionAnti-HLA-B27 Antibody, clone HLA.ABC.m3
      OverviewBIOCHEMISTRY: pI = 8.0
      References
      Product Information
      FormatPurified
      PresentationThe antibody is supplied in phosphate-buffered saline, pH 7.4, containing 0.2% bovine serum albumin and 0.1% sodium azide. The characteristics of each lot are tested by flow cytometry.
      Quality LevelMQ100
      Applications
      ApplicationThis Anti-HLA-B27 Antibody, clone HLA.ABC.m3 is validated for use in FC, IC for the detection of HLA-B27.
      Key Applications
      • Flow Cytometry
      • Immunocytochemistry
      Application NotesThis antibody is useful in the investigation of HLA-B27 in diseases such as ankylosing spondylitis and anterior uveitis.

      SUGGESTED USAGE DILUTION

      1. Flow cytometry and indirect immunofluorescence 1:60 to 1:120

      Dilute with isotonic buffer. Use 50 μl of diluted antibody per 1 x 10E6 peripheral blood mononuclear cells (PBMC) in 100 μl buffer.

      2. Cytotoxicity testing 1:25.
      Biological Information
      ImmunogenImmune complex precipitated from HLA-B27 positive cell line (Bordin) by anti HLA antibody and staphylococcal Protein A.
      CloneHLA.ABC.m3
      ConcentrationPlease refer to the Certificate of Analysis for the lot-specific concentration.
      HostMouse
      SpecificityThis antibody binds strongly to the HLA-B27 antigen. It also binds less strongly to the HLA-B7 antigen. Affinity studies by Scatchard analysis showed that the antibody has a higher affinity for HLA-B27 (9.7x108M-1) than for HLA-B7

      (9.5x107M-1).

      Cell reactivity:

      Flow cytometric analysis of lymphocyte staining for HLA-B27 gave the following results:



      Subjects Fluorescence Intensity

      Heterozygous HLA-B27+ Strong

      Homozygous HLA-B7+ Strong

      Heterozygous HLA-B7+ Faint

      Non B27, Non B7 Negative
      IsotypeIgG2a
      Species Reactivity
      • Human
      Antibody TypeMonoclonal Antibody
      Entrez Gene Number
      Entrez Gene SummaryHLA-B belongs to the HLA class I heavy chain paralogues. This class I molecule is a heterodimer consisting of a heavy chain and a light chain (beta-2 microglobulin). The heavy chain is anchored in the membrane. Class I molecules play a central role in the immune system by presenting peptides derived from the endoplasmic reticulum lumen. They are expressed in nearly all cells. The heavy chain is approximately 45 kDa and its gene contains 8 exons. Exon 1 encodes the leader peptide, exon 2 and 3 encode the alpha1 and alpha2 domains, which both bind the peptide, exon 4 encodes the alpha3 domain, exon 5 encodes the transmembrane region and exons 6 and 7 encode the cytoplasmic tail. Polymorphisms within exon 2 and exon 3 are responsible for the peptide binding specificity of each class one molecule. Typing for these polymorphisms is routinely done for bone marrow and kidney transplantation. Hundreds of HLA-B alleles have been described.
      Gene Symbol
      • HLA-B
      • Bw-54
      • Bw-45
      • Bw-47
      • Bw-46
      • Bw-52
      • B-5
      • Bw-4
      • Bw-57
      • OTTHUMP00000034826
      • AS
      • Bw-56
      • Bw-55
      • Bw-41
      • Bw-48
      • B'DT
      • Bw-50
      • Bw-60
      • Bw-58
      • B-21
      • HLA-B-5501
      • B-12
      • Bw-22
      • Bw-53
      • HLAB
      • SPDA1
      • Bw-44
      UniProt Number
      UniProt SummaryFUNCTION: SwissProt: P30498 # Involved in the presentation of foreign antigens to the immune system.| Q31612 # Involved in the presentation of foreign antigens to the immune system.| P30488 # Involved in the presentation of foreign antigens to the immune system.| P03989 # Involved in the presentation of foreign antigens to the immune system.| P30493 # Involved in the presentation of foreign antigens to the immune system.| P30460 # Involved in the presentation of foreign antigens to the immune system.| P30484 # Involved in the presentation of foreign antigens to the immune system.| P01889 # Involved in the presentation of foreign antigens to the immune system.| P30485 # Involved in the presentation of foreign antigens to the immune system.| Q29940 # Involved in the presentation of foreign antigens to the immune system.| P30483 # Involved in the presentation of foreign antigens to the immune system.| P30495 # Involved in the presentation of foreign antigens to the immune system.| P30492 # Involved in the presentation of foreign antigens to the immune system.| P18465 # Involved in the presentation of foreign antigens to the immune system.| P30487 # Involved in the presentation of foreign antigens to the immune system.| P30480 # Involved in the presentation of foreign antigens to the immune system.| P30491 # Involved in the presentation of foreign antigens to the immune system.| Q95365 # Involved in the presentation of foreign antigens to the immune system.| Q29836 # Involved in the presentation of foreign antigens to the immune system.| Q29718 # Involved in the presentation of foreign antigens to the immune system.| P30475 # Involved in the presentation of foreign antigens to the immune system.| P18464 # Involved in the presentation of foreign antigens to the immune system.| P30479 # Involved in the presentation of foreign antigens to the immune system.| P30490 # Involved in the presentation of foreign antigens to the immune system.| P10319 # Involved in the presentation of foreign antigens to the immune system.| P30466 # Involved in the presentation of foreign antigens to the immune system.| P30461 # Involved in the presentation of foreign antigens to the immune system.| P18463 # Involved in the presentation of foreign antigens to the immune system.| Q04826 # Involved in the presentation of foreign antigens to the immune system.| P30481 # Involved in the presentation of foreign antigens to the immune system.| P30685 # Involved in the presentation of foreign antigens to the immune system.| P30462 # Involved in the presentation of foreign antigens to the immune system.| Q31610 # Involved in the presentation of foreign antigens to the immune system.| P30486 # Involved in the presentation of foreign antigens to the immune system.| P30464 # Involved in the presentation of foreign antigens to the immune system.


      SIZE: 362 amino acids; 40478 Da

      SUBUNIT: Heterodimer of an alpha chain and a beta chain (beta-2- microglobulin). Interacts with human herpesvirus 8 MIR1 protein (By similarity).

      SUBCELLULAR LOCATION: Membrane; Single-pass type I membrane protein.



      DOMAIN: SwissProt: P30498

      PTM: Polyubiquitinated in a post ER compartment by interaction with human herpesvirus 8 MIR1 protein. This targets the protein for rapid degradation via the ubiquitin system (By similarity).

      DISEASE: "SwissProt: P03989 # HLA-B27 is associated with the development of ankylosing spondylitis (AS) [MIM:106300]. AS is a chronic inflammatory rheumatic disease that mainly affects the axial skeleton and is considered the prototype of seronegative spondyloarthropathies (SNSA), which include reactive arthritis (e.g., Reiter's syndrome), psoritic spondylitis, and other spondyloarthropathies (SpA). In the Greek Cypriot population, a restricted number of HLA-B27 subtypes are associated with AS and other B27-related diseases and an elevated frequency of the B*2702 allele in the AS patients is identified. The allele B*2707 seems to have a protective role in this population because it was found only in the healthy controls.| P18464 # HLA-B51 is associated with Behcet disease but it is not certain whether HLA-B51 itself or a closely linked gene is responsible for susceptibility. About 20% of healthy individuals of various ethnic origin carries the HLA-B 51 locus, compared with 50 to 80% of patients. Behcet disease is a systemic disorder of recurrent acute inflammation characterized by 4 major symptoms: oral aphthous ulcers, skin lesions, ocular symptoms and genital ulcerations. Occasionally, inflammation in tissues and organs throughout the body can occur, including the gastrointestinal tract, central nervous system, vascular system, lungs, and kidneys. The etiology of Behcet disease is unclear.| P30464 # Involved in susceptibility to Stevens-Johnson syndrome [MIM:608579]. This is a life-threatening reaction of the skin to particular types of medication. It is characterized by high fever, malaise and blistering exanthema. The more severe form of the disease is referred to as toxic epidermal necrolysis."

      SIMILARITY: SwissProt: P30498 ## Belongs to the MHC class I family. & Contains 1 Ig-like C1-type (immunoglobulin-like) domain. | Q31612 ## Belongs to the MHC class I family. & Contains 1 Ig-like C1-type (immunoglobulin-like) domain. | P30488 ## Belongs to the MHC class I family. & Contains 1 Ig-like C1-type (immunoglobulin-like) domain. | P03989 ## Belongs to the MHC class I family. & Contains 1 Ig-like C1-type (immunoglobulin-like) domain. | P30493 ## Belongs to the MHC class I family. & Contains 1 Ig-like C1-type (immunoglobulin-like) domain. | P30460 ## Belongs to the MHC class I family. & Contains 1 Ig-like C1-type (immunoglobulin-like) domain. | P30484 ## Belongs to the MHC class I family. & Contains 1 Ig-like C1-type (immunoglobulin-like) domain. | P01889 ## Belongs to the MHC class I family. & Contains 1 Ig-like C1-type (immunoglobulin-like) domain. | P30485 ## Belongs to the MHC class I family. & Contains 1 Ig-like C1-type (immunoglobulin-like) domain. | Q29940 ## Belongs to the MHC class I family. & Contains 1 Ig-like C1-type (immunoglobulin-like) domain. | P30483 ## Belongs to the MHC class I family. & Contains 1 Ig-like C1-type (immunoglobulin-like) domain. | P30495 ## Belongs to the MHC class I family. & Contains 1 Ig-like C1-type (immunoglobulin-like) domain. | P30492 ## Belongs to the MHC class I family. & Contains 1 Ig-like C1-type (immunoglobulin-like) domain. | P18465 ## Belongs to the MHC class I family. & Contains 1 Ig-like C1-type (immunoglobulin-like) domain. | P30487 ## Belongs to the MHC class I family. & Contains 1 Ig-like C1-type (immunoglobulin-like) domain. | P30480 ## Belongs to the MHC class I family. & Contains 1 Ig-like C1-type (immunoglobulin-like) domain. | P30491 ## Belongs to the MHC class I family. & Contains 1 Ig-like C1-type (immunoglobulin-like) domain. | Q95365 ## Belongs to the MHC class I family. & Contains 1 Ig-like C1-type (immunoglobulin-like) domain. | Q29836 ## Belongs to the MHC class I family. & Contains 1 Ig-like C1-type (immunoglobulin-like) domain. | Q29718 ## Belongs to the MHC class I family. & Contains 1 Ig-like C1-type (immunoglobulin-like) domain. | P30475 ## Belongs to the MHC class I family. & Contains 1 Ig-like C1-type (immunoglobulin-like) domain. | P18464 ## Belongs to the MHC class I family. & Contains 1 Ig-like C1-type (immunoglobulin-like) domain. | P30479 ## Belongs to the MHC class I family. & Contains 1 Ig-like C1-type (immunoglobulin-like) domain. | P30490 ## Belongs to the MHC class I family. & Contains 1 Ig-like C1-type (immunoglobulin-like) domain. | P10319 ## Belongs to the MHC class I family. & Contains 1 Ig-like C1-type (immunoglobulin-like) domain. | P30466 ## Belongs to the MHC class I family. & Contains 1 Ig-like C1-type (immunoglobulin-like) domain. | P30461 ## Belongs to the MHC class I family. & Contains 1 Ig-like C1-type (immunoglobulin-like) domain. | P18463 ## Belongs to the MHC class I family. & Contains 1 Ig-like C1-type (immunoglobulin-like) domain. | Q04826 ## Belongs to the MHC class I family. & Contains 1 Ig-like C1-type (immunoglobulin-like) domain. | P30481 ## Belongs to the MHC class I family. & Contains 1 Ig-like C1-type (immunoglobulin-like) domain. | P30685 ## Belongs to the MHC class I family. & Contains 1 Ig-like C1-type (immunoglobulin-like) domain. | P30462 ## Belongs to the MHC class I family. & Contains 1 Ig-like C1-type (immunoglobulin-like) domain. | Q31610 ## Belongs to the MHC class I family. & Contains 1 Ig-like C1-type (immunoglobulin-like) domain. | P30486 ## Belongs to the MHC class I family. & Contains 1 Ig-like C1-type (immunoglobulin-like) domain. | P30464 ## Belongs to the MHC class I family. & Contains 1 Ig-like C1-type (immunoglobulin-like) domain.
      Physicochemical Information
      Dimensions
      Materials Information
      Toxicological Information
      Safety Information according to GHS
      Safety Information
      Product Usage Statements
      Usage Statement
      • Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
      Storage and Shipping Information
      Storage ConditionsStore at 2 to 8°C. For prolonged periods, store below -20°C in undiluted aliquots. AVOID REPEATED FREEZE/THAW CYCLES.

      WARNING: The monoclonal reagent solution contains 0.1% sodium azide as a preservative. Due to potential hazards arising from the build up of this material in pipes, spent reagent should be disposed of with liberal volumes of water.
      Packaging Information
      Material Size100 µg
      Transport Information
      Supplemental Information
      Specifications
      Global Trade Item Number
      Catalogue Number GTIN
      MAB1285 04053252264511

      Documentation

      Anti-HLA-B27 Antibody, clone HLA.ABC.m3 SDS

      Title

      Safety Data Sheet (SDS) 

      Anti-HLA-B27 Antibody, clone HLA.ABC.m3 Certificates of Analysis

      TitleLot Number
      ANTI-HLA-B27, CLN HLA.ABC.M3 MOUSE MONOCLONAL ANTIBODY CERTIFICATE OF ANALYSIS - 2164951 2164951
      ANTI-HLA-B27, CLN HLA.ABC.M3 MOUSE MONOCLONAL ANTIBODY - 2441961 2441961
      ANTI-HLA-B27, CLN HLA.ABC.M3 - 2219358 2219358
      ANTI-HLA-B27, CLN HLA.ABC.M3 - 3288689 3288689
      ANTI-HLA-B27, CLN HLA.ABC.M3 - 3382293 3382293
      ANTI-HLA-B27, CLN HLA.ABC.M3 - 3428802 3428802
      ANTI-HLA-B27, CLN HLA.ABC.M3 - 3519302 3519302
      ANTI-HLA-B27, CLN HLA.ABC.M3 - 3574406 3574406
      ANTI-HLA-B27, CLN HLA.ABC.M3 - 3590789 3590789
      ANTI-HLA-B27, CLN HLA.ABC.M3 - 3594290 3594290
      Show more

      References

      Reference overviewPub Med ID
      Description of a mouse monoclonal anti-HLA-B27 antibody HLA-ABC-m3.
      Trapani, J A, et al.
      Hum. Immunol., 7: 205-16 (1983)  1983

      Show Abstract       		6604046 6604046

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      Categories

      Life Science Research > Antibodies and Assays > Primary Antibodies