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ABS2053-25UG Anti-Glutaredoxin-2 (Grx2)

Anti-Glutaredoxin-2 (Grx2), Cat. No. ABS2053, is a rabbit polyclonal antibody that detects Glutaredoxin-2, mitochondrial, and has been tested for use in Immunohistochemistry (Paraffin) and Western Blotting.

Anti-Glutaredoxin-2 (Grx2) MSDS (material safety data sheet) or SDS, CoA and CoQ, dossiers, brochures and other available documents.
Research Category: Signaling Gene Symbol: GLRX2, GRX2, CGI-133 UniProt Number: Q9NS18
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ABS2053-25UG
25 μg  
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      Overview

      Replacement Information

      Key Spec Table

      Species ReactivityKey ApplicationsHostFormatAntibody Type
      HWB, IH(P)RbAffinity PurifiedPolyclonal Antibody
      Description
      Catalogue NumberABS2053-25UG
      DescriptionAnti-Glutaredoxin-2 (Grx2)
      Alternate Names
      • Glutaredoxin-2
      • mitochondrial
      Background InformationGlutaredoxin-2, mitochondrial (UniProt: Q9NS18) is encoded by the GLRX2 (also known as GRX2, CGI-133) gene (Gene ID: 51022) in human. Glutaredoxin-2 is a glutathione-dependent oxidoreductase that is widely expressed and facilitates the maintenance of mitochondrial redox homeostasis upon induction of apoptosis by oxidative stress. Its active form is a monomer and it can dimerize to an inactive form where homodimers are linked by one 2Fe-2S cluster. The 2Fe-2S may serve as a redox sensor and the presence of one-electron oxidants or reductants can lead to the loss of the 2Fe-2S cluster and subsequent monomerization and activation of the enzyme. Glutaredoxin-2 acts as a very efficient catalyst of monothiol reactions because of its high affinity for protein glutathione-mixed disulfides. It can receive electrons not only from glutathione (GSH), but also from thioredoxin reductase supporting both monothiol and dithiol reactions. Glutaredoxin-2 is known to efficiently catalyze both glutathionylation and deglutathionylation of mitochondrial complex I, which in turn regulates the superoxide production by the complex. Overexpression of glutaredoxin-2 decreases the susceptibility to apoptosis and prevents loss of cardiolipin and cytochrome c release.
      References
      Product Information
      FormatAffinity Purified
      PresentationPurified rabbit polyclonal antibody in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide.
      Applications
      ApplicationAnti-Glutaredoxin-2 (Grx2), Cat. No. ABS2053, is a rabbit polyclonal antibody that detects Glutaredoxin-2, mitochondrial, and has been tested for use in Immunohistochemistry (Paraffin) and Western Blotting.
      Key Applications
      • Western Blotting
      • Immunohistochemistry (Paraffin)
      Application NotesImmunohistochemistry Analysis: A 1:1,000 dilution from a representative lot detected Glutaredoxin-2 (Grx2) in human cerebellum and human tonsil tissue.
      Biological Information
      ImmunogenKLH-conjugated linear peptide corresponding to 16 amino acids from the C-terminal half of human Glutaredoxin-2 .
      ConcentrationPlease refer to lot specific datasheet.
      HostRabbit
      SpecificityThis rabbit polyclonal antibody detects mitochondrial isoform of glutatedoxin-2. It targets an epitope with in 16 amino acids from the C-terminal half.
      Species Reactivity
      • Human
      Species Reactivity NoteHuman. Predicted to react with Chimpanzee, Baboon, Monkey based on 100% sequence homology.
      Antibody TypePolyclonal Antibody
      Gene Symbol
      • GLRX2
      • GRX2
      • CGI-133
      Purification MethodAffinity Purified
      UniProt Number
      Molecular Weight17 kDa observed. Uncharacterized bands may be observed in some lysate(s).
      Physicochemical Information
      Dimensions
      Materials Information
      Toxicological Information
      Safety Information according to GHS
      Safety Information
      Product Usage Statements
      Quality AssuranceEvaluated by Western Blotting in A549 cell lysate.
      Western Blotting Analysis: 0.5 µg/mL of this antibody detected Glutaredoxin-2 (Grx2) in 10 µg of A549 cell lysate.
      Usage Statement
      • Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
      Storage and Shipping Information
      Storage ConditionsStable for 1 year at 2-8°C from date of receipt.
      Packaging Information
      Material Size25 μg
      Transport Information
      Supplemental Information
      Specifications
      Global Trade Item Number
      Catalogue Number GTIN
      ABS2053-25UG 04054839499272