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DR1067 Anti-GTF2I Mouse mAb (3E2)

DR1067
  
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      Overview

      Key Spec Table

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      M
      Description
      Overview

      This product has been discontinued.





      Recognizes the ~130 kDa GTF2I protein in HeLa cells and human colon and placenta tissue.
      Catalogue NumberDR1067
      Brand Family Calbiochem®
      SynonymsAnti-General Transcription Factor IIi
      Application Data
      Detection of human GTF2I by immunoblotting. Sample: Whole tissue extract from human colon. Primary antibody: Anti-GTF2I Mouse mAb (3E2) (Cat. No. DR1067) (1-5 µg/ml). Detection: chemiluminescence.

      Detection of human GTF2I by immunocytochemistry. Sample: HeLa cells fixed in paraformaldehyde. Primary antibody: Anti-GTF2I Mouse mAb (3E2) (Cat. No. DR1067) (10 µg/ml). Detection: fluorescence.

      Detection of human GTF2I by staining paraffin sections. Sample: Formalin-fixed, paraffin-embedded human placenta tissue. Primary antibody: Anti-GTF2I Mouse mAb (3E2) (Cat. No. DR1067) (3 µg/ml). Detection: colorimetric (DAB).
      Product Information
      FormLiquid
      FormulationIn PBS, pH 7.11.
      Positive controlHuman colon tissue, Human placenta tissue, HeLa cells
      PreservativeNone
      Applications
      Key Applications Enzyme-Linked Immunosorbent Assay
      Immunoblotting (Western Blotting)
      Immunocytochemistry
      Paraffin Sections
      Application NotesELISA (1-5 µg/ml)
      Immunoblotting (1-5 µg/ml)
      Immunocytochemistry (10 µg/ml)
      Paraffin Sections (3 µg/ml)
      Application CommentsFor ELISA, this antibody can be used as the capture antibody; recommended concentration is 10 ng/ml, 1 µg/well, 100 µl total volume. Variables associated with assay conditions will dictate the proper working dilution.
      Biological Information
      ImmunogenFull-length, recombinant, human GTF2l (aa 36-275), expressed as a GST fusion protein
      Clone3E2
      HostMouse
      IsotypeIgG2a
      Storage and Shipping Information
      Ship Code Blue Ice Only
      Toxicity Regulatory Review
      Storage -20°C
      Avoid freeze/thaw Avoid freeze/thaw
      Do not freeze Ok to freeze
      Special InstructionsFollowing initial thaw, aliquot and freeze (-20°C).
      Global Trade Item Number
      Catalogue Number GTIN
      DR1067 0