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MABT1539-25UG Anti-Filaggrin Antibody, clone AHF3

This mouse monoclonal Anti-Filaggrin, clone AHF3, Cat. No. MABT1539 is tested for use in Immunocytochemistry, ELISA, Immunofluorescence, Electron Microscopy, Western Blotting and Immunohistochemistry,

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      Overview

      Description
      Catalogue NumberMABT1539-25UG
      DescriptionAnti-Filaggrin Antibody, clone AHF3
      Alternate Names
      • FLG
      Background InformationFilaggrin (UniProt: P20930; also known as FLG) is encoded by the FLG gene (Gene ID: 2312) in human. Filaggrin, a calcium-binding protein, is synthesized as a large, insoluble, highly phosphorylated precursor with several tandem copies of 324 amino acids in cytoplasmic granules. During terminal differentiation it undergoes dephosphorylation and proteolytic cleavage to remove linker peptides and produce functional basic filaggrin units. It is widely expressed and in skin tissue it is detected mainly in stratum granulosum of the epidermis. During terminal differentiation of mammalian epidermis, it aggregates keratin intermediate filaments and promotes disulfide-bond formation among the intermediate filaments. This creates the intracellular fibrous matrix of the cornified cells. Deimination of arginine to citrulline by peptidylarginine deiminase is reported to result in reduction in its affinity for cytokeratins. The N-terminal region of the mature filaggrin is shown to be heterogenous and is blocked by the formation of pyroglutamate. Clone AHF3 detects both the profilaggrin and filaggrin and exhibits reactivity in various immunochemical applications. (Ref.: Simon, M., et al. (1996). Biochem. J. 317(pt 1); 173 177; Simon, M., et al. (1995). Clin. Exp. Immunol. 100(1); 90-98; Simon, M., et al. (1995). J. Invest. Dermatol. 105(3); 432-437).
      Product Information
      FormatPurified
      PresentationPurified mouse monoclonal antibodyIgG1κ in buffer containing PBS with 0.1% sodium azide with 50% glycerol.
      Quality LevelMQ200
      Applications
      ApplicationThis mouse monoclonal Anti-Filaggrin, clone AHF3, Cat. No. MABT1539 is tested for use in Immunocytochemistry, ELISA, Immunofluorescence, Electron Microscopy, Western Blotting and Immunohistochemistry, for the detection of filaggrin and pro-filaggrin..
      Key Applications
      • Immunocytochemistry
      • Immunofluorescence
      • Immunohistochemistry
      • ELISA
      • Western Blotting
      • Electron Microscopy
      Application NotesTested Applications

      Immunohistochemistry Applications: A representative lot detected Filaggrin in Immunohistochemistry application.. (Henry, J., et al. (2011). FASEB J. 25(5):1567-76; Hsu, C-Y., et al. (2011). J Biol Chem. 286(26):23222-33).

      Western Blotting Analysis: A representative lot detected Filaggrin in Western Blotting application. (Simon, M., et al. (1995). J Invest Dermatol. 105(3):432-7; Simon, M., et al. (1996). Biochem J. 317(Pt1)(Pt1):173-7; Henry, J., et al. (2011). FASEB J. 25(5):1567-76; Hsu, C-Y., et al. (2011). J Biol Chem. 286(26):23222-33; Pellerin, L., et al. (2013). J Allergy Clin Immunol. 131(4):1094-102; Hsu, C-Y., et al. (2017). J Invest Dermatol. 137(2):422-429).

      Electron Microscopy: A representative lot detected Filaggrin in Electron Microscopy application. (Simon, M., et al. (1995). J Invest Dermatol. 105(3):432-7; Henry, J., et al. (2011). FASEB J. 25(5):1567-76).

      Immunofluorescence Analysis: A representative lot detected Filaggrin in Immunofluorscence application. (Simon, M., et al. (1995). J Invest Dermatol. 105(3):432-7; Henry, J., et al. (2011). FASEB J. 25(5):1567-76; Hsu, C-Y., et al. (2011). J Biol Chem. 286(26):23222-33).

      ELISA Analysis: A representative lot detected Filaggrin in ELISA application. (Simon, M., et al. (1995). Clin Exp Immunol. 100(1):90-8; Simon, M., et al. (1996). Biochem J. 317(Pt1)(Pt1):173-7; Miedouge, M., et al. (2001). Tumour Biol. 22(1):19-26).

      Note: Actual optimal working dilutions must be determined by end user as specimens, and experimental conditions may vary with the end user.
      Biological Information
      ImmunogenPartially purified Filaggrin from human breast skin tissue.
      EpitopeUnknown
      CloneAHF3
      Concentration1.0 mg/mL. Please refer to guidance on suggested starting dilutions and/or titers per application and sample type.
      HostMouse
      SpecificityClone AHF3 is a mouse monoclonal antibody that detects Filaggrin and Profilaggrin.
      IsotypeIgG1κ
      Species Reactivity
      • Human
      Species Reactivity NoteHuman.
      Antibody TypeMonoclonal Antibody
      Entrez Gene Number
      Gene Symbol
      • FLG
      Purification MethodDEAE
      UniProt Number
      Molecular Weight435.17 kDa calculated.
      Product Usage Statements
      Quality AssuranceEvaluated by Immunocytochemistry in HaCat cells.

      Immunocytochemistry Analysis: A 1:1,000 dilution of this antibody detected Filaggrin in HaCat cells.
      Usage Statement
      • Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
      Storage and Shipping Information
      Storage ConditionsStore at -10°C to -25°C. Handling Recommendations: Upon receipt and prior to removing the cap, centrifuge the vial and gently mix the solution. Aliquot into microcentrifuge tubes and store at -20°C. Avoid repeated freeze/thaw cycles, which may damage IgG and affect product performance.
      Packaging Information
      Material Size25 μg
      Global Trade Item Number
      Catalogue Number GTIN
      MABT1539-25UG 04065271270176