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PC168 Anti-Estrogen Receptor β (Ab-1) (485-503) Rabbit pAb

This Anti-Estrogen Receptor β Rabbit pAb is validated for use in Frozen Sections, Gel Shift, WB, Paraffin Sections for the detection of Estrogen Receptor β.

MSDS (material safety data sheet) or SDS, CoA and CoQ, dossiers, brochures and other available documents.

Synonyms: Anti-ER β

PC168
  
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      Overview

      Replacement Information

      Key Spec Table

      Species ReactivityHostAntibody Type
      M, RRbPolyclonal Antibody
      Description
      OverviewRecognizes the ~60 kDa estrogen receptor β protein in overexpressing cell lines and breast carcinoma tissue. Does not cross-react with estrogen receptor α.
      Catalogue NumberPC168
      Brand Family Calbiochem®
      SynonymsAnti-ER β
      Application Data
      Detection of mouse estrogen receptor β by immunoblotting. Samples: I-11.15 cells (lane A), F9 cells (lane B) and NIH/3T3 cells (lane C) and whole cell lysates from mouse thymus cells (lane D) and spleen (lane E). Primary antibody: Anti-Estrogen Receptor β (Ab-1) (485-503) Rabbit pAb (Cat. No. PC168) (1:1000). Detection: chemiluminescence.
      References
      ReferencesHussien, A., et al. 1997. Proc. Natl. Acad. Sci. USA 94, 1521.
      Li, X., et al. 1997. Neuroendocrinology 66, 63.
      Pratt, W.B. and Toft, D.O. 1997. Endocrine Rev. 18, 306.
      Schumacher, M., et al. 1996. Dev. Neurosci. 18, 6.
      Toran-Allerand, C.D. 1996. Dev. Neurosci. 18, 36.
      Weigel, N.L. 1996. Biochem. J. 319, 657.
      Jones, K.J. 1994. Ann. N.Y. Acad. Sci. 743, 141.
      Arnold, A.P. 1990. in Hormones, Brain and Behavior in Vertebrates, ed. Balthazart, J. (Karger, Basel), pp. 82-91.
      Product Information
      FormLiquid
      FormulationIn PBS, 1 mg/ml BSA.
      Positive controlBreast carcinoma tissue
      Preservative≤0.1% sodium azide
      Applications
      Key Applications Frozen Sections
      Gel Shift
      Immunoblotting (Western Blotting)
      Paraffin Sections
      Application NotesFrozen Sections (5-10 µg/ml)
      Gel Shift (1-5 µl)
      Immunoblotting (1 µg/ml)
      Paraffin Sections (1:50)
      Application CommentsThis antibody will also specifically and efficiently supershift rat estrogen receptor β. Does not cross-react with estrogen receptor α. Antibody should be titrated for optimal results in individual systems.
      Biological Information
      Immunogena synthetic peptide (CSSTEDSKNKESSQNLQSQ) corresponding to amino acids 485-503 of rat estrogen receptor β
      ImmunogenRat
      HostRabbit
      IsotypeIgG
      Species Reactivity
      • Mouse
      • Rat
      Antibody TypePolyclonal Antibody
      Concentration Label Please refer to vial label for lot-specific concentration
      Physicochemical Information
      Dimensions
      Materials Information
      Toxicological Information
      Safety Information according to GHS
      Safety Information
      Product Usage Statements
      Storage and Shipping Information
      Ship Code Dry Ice Only
      Toxicity Standard Handling
      Storage -20°C
      Avoid freeze/thaw Avoid freeze/thaw
      Do not freeze Ok to freeze
      Special InstructionsFollowing initial thaw, aliquot and freeze (-20°C).
      Packaging Information
      Transport Information
      Supplemental Information
      Specifications
      Global Trade Item Number
      Catalogue Number GTIN
      PC168 0

      Documentation

      Anti-Estrogen Receptor β (Ab-1) (485-503) Rabbit pAb SDS

      Title

      Safety Data Sheet (SDS) 

      Anti-Estrogen Receptor β (Ab-1) (485-503) Rabbit pAb Certificates of Analysis

      TitleLot Number
      PC168

      References

      Reference overview
      Hussien, A., et al. 1997. Proc. Natl. Acad. Sci. USA 94, 1521.
      Li, X., et al. 1997. Neuroendocrinology 66, 63.
      Pratt, W.B. and Toft, D.O. 1997. Endocrine Rev. 18, 306.
      Schumacher, M., et al. 1996. Dev. Neurosci. 18, 6.
      Toran-Allerand, C.D. 1996. Dev. Neurosci. 18, 36.
      Weigel, N.L. 1996. Biochem. J. 319, 657.
      Jones, K.J. 1994. Ann. N.Y. Acad. Sci. 743, 141.
      Arnold, A.P. 1990. in Hormones, Brain and Behavior in Vertebrates, ed. Balthazart, J. (Karger, Basel), pp. 82-91.
      Data Sheet

      Note that this data sheet is not lot-specific and is representative of the current specifications for this product. Please consult the vial label and the certificate of analysis for information on specific lots. Also note that shipping conditions may differ from storage conditions.

      Revision08-October-2007 RFH
      SynonymsAnti-ER β
      ApplicationFrozen Sections (5-10 µg/ml)
      Gel Shift (1-5 µl)
      Immunoblotting (1 µg/ml)
      Paraffin Sections (1:50)
      Application Data
      Detection of mouse estrogen receptor β by immunoblotting. Samples: I-11.15 cells (lane A), F9 cells (lane B) and NIH/3T3 cells (lane C) and whole cell lysates from mouse thymus cells (lane D) and spleen (lane E). Primary antibody: Anti-Estrogen Receptor β (Ab-1) (485-503) Rabbit pAb (Cat. No. PC168) (1:1000). Detection: chemiluminescence.
      DescriptionImmunoaffinity purified rabbit polyclonal antibody. Recognizes the ~60 kDa estrogen receptor beta protein.
      BackgroundSteroids regulate important neuronal functions including the organization of neural circuits during development and the regulation of synapse organization in the adult. Steroid hormones may promote cell survival and differentiation of both neurons and glial cells, regulate synaptogenesis, prevent synapse elimination, cause axonal and dendritic growth, and play a role during regeneration of the nervous system. Steroid hormone receptors are members of a large family of nuclear ligand-activated transcription factors that includes the androgen, estrogen, glucocorticoid, and progesterone receptors. In the absence of hormone, steroid receptors are associated in complexes with heat shock proteins and immunophilins. Steroid binding results in the dissociation of the heat-shock protein, receptor dimerization, and binding to specific hormone response elements (HRE) to produce a transcriptionally active complex that leads to the activation or repression of target genes. Steroid receptors are regulated by phosphorylation, however, modulation of kinase activity can also cause receptor activation in the absence of hormone. Recent evidence suggests that steroid receptor expression can be regulated by neurotrophins, and that steroids can regulate the expression of trophic factors and their receptors. Estrogen receptor Beta has been shown to be a high affinity estrogen binding protein capable of initiating transcription of genes under the control of estrogen response elements. RNA distribution studies have shown that estrogen receptor beta is transcribed in human thymus, spleen, ovary and testis as well as rat ovary and prostate.
      HostRabbit
      Immunogen speciesRat
      Immunogena synthetic peptide (CSSTEDSKNKESSQNLQSQ) corresponding to amino acids 485-503 of rat estrogen receptor β
      IsotypeIgG
      Speciesmouse, rat
      Positive controlBreast carcinoma tissue
      FormLiquid
      FormulationIn PBS, 1 mg/ml BSA.
      Concentration Label Please refer to vial label for lot-specific concentration
      Preservative≤0.1% sodium azide
      CommentsThis antibody will also specifically and efficiently supershift rat estrogen receptor β. Does not cross-react with estrogen receptor α. Antibody should be titrated for optimal results in individual systems.
      Storage Avoid freeze/thaw
      -20°C
      Do Not Freeze Ok to freeze
      Special InstructionsFollowing initial thaw, aliquot and freeze (-20°C).
      Toxicity Standard Handling
      ReferencesHussien, A., et al. 1997. Proc. Natl. Acad. Sci. USA 94, 1521.
      Li, X., et al. 1997. Neuroendocrinology 66, 63.
      Pratt, W.B. and Toft, D.O. 1997. Endocrine Rev. 18, 306.
      Schumacher, M., et al. 1996. Dev. Neurosci. 18, 6.
      Toran-Allerand, C.D. 1996. Dev. Neurosci. 18, 36.
      Weigel, N.L. 1996. Biochem. J. 319, 657.
      Jones, K.J. 1994. Ann. N.Y. Acad. Sci. 743, 141.
      Arnold, A.P. 1990. in Hormones, Brain and Behavior in Vertebrates, ed. Balthazart, J. (Karger, Basel), pp. 82-91.