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CP18 Anti-Collagen Type II (Ab-1) Mouse mAb (II-4C11)

This Anti-Collagen Type II (Ab-1) Mouse mAb (II-4C11) is validated for use in Frozen Sections for the detection of Collagen Type II (Ab-1).

MSDS (material safety data sheet) or SDS, CoA and CoQ, dossiers, brochures and other available documents.
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CP18
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Overview

Replacement Information

Key Spec Table

Species ReactivityHostAntibody Type
B, H, Rb, RMMonoclonal Antibody

Pricing & Availability

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CP18-100UG
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      Description
      OverviewRecognizes the ~130 kDa type II collagen protein. May react with other collagens. When used at high concentrations higher bands corresponding to aggregates may also be detected.
      Catalogue NumberCP18
      Brand Family Calbiochem®
      References
      ReferencesKumagai, J. et al. 1994 J. Anat. 185, 278.
      Kivrikko, K,I. 1993 Ann. Med. 25, 113.
      Sandell, L.J., et al. 1991 J. Cell. Biol. 114, 1307.
      Ryan, M.C., and Sandell, L.J. 1990 J. Biol. Chem. 265, 10334.
      Mayne, R., and Burgeson, R.E. 1987 Structure and Function of Collagen Types,Academic Press, Orlando, FL
      Product Information
      DeclarationManufactured by Daiichi Fine Chemical Co., Ltd. Not available for sale in Japan.
      FormLiquid
      FormulationIn 100 mM sodium phosphate buffer, 0.1% BSA, pH 7.0.
      Preservative≤0.1% sodium azide
      Quality LevelMQ100
      Applications
      Application ReferencesImmunoblotting Kumagai, J. et al. 1994 J. Anat. 185, 278. Paraffin Sections Kumagai, J. et al. 1994 J. Anat. 185, 278.
      Key Applications Frozen Sections
      Immunoblotting (Western Blotting)
      Paraffin Sections
      Application NotesFrozen Sections (1-10 µg/ml)
      Immunoblotting (1-5 µg/ml, see application references)
      Paraffin Sections (1-10 µg/ml, see application references)
      Application CommentsMay react with other collagens. When used at high concentrations higher bands corresponding to aggregates may also be detected. This antibody may also react with other classes of collagen at a concentration higher than 1 µg/ml. In immunoblotting, a major band of ~130 kDa is obtained. Antibody should be titrated for optimal results in individual systems.
      Biological Information
      Immunogenpurified collagen type II
      ImmunogenHuman
      CloneII-4C11
      HostMouse
      IsotypeIgG₁
      Species Reactivity
      • Bovine
      • Human
      • Rabbit
      • Rat
      Antibody TypeMonoclonal Antibody
      Concentration Label Please refer to vial label for lot-specific concentration
      Physicochemical Information
      Dimensions
      Materials Information
      Toxicological Information
      Safety Information according to GHS
      Safety Information
      Product Usage Statements
      Storage and Shipping Information
      Ship Code Blue Ice Only
      Toxicity Standard Handling
      Storage -20°C
      Avoid freeze/thaw Avoid freeze/thaw
      Do not freeze Ok to freeze
      Special InstructionsFollowing initial thaw, aliquot and freeze (-20°C).
      Packaging Information
      Transport Information
      Supplemental Information
      Specifications
      Global Trade Item Number
      Catalogue Number GTIN
      CP18-100UG 04055977220957

      Documentation

      Anti-Collagen Type II (Ab-1) Mouse mAb (II-4C11) SDS

      Title

      Safety Data Sheet (SDS) 

      Anti-Collagen Type II (Ab-1) Mouse mAb (II-4C11) Certificates of Analysis

      TitleLot Number
      CP18

      References

      Reference overview
      Kumagai, J. et al. 1994 J. Anat. 185, 278.
      Kivrikko, K,I. 1993 Ann. Med. 25, 113.
      Sandell, L.J., et al. 1991 J. Cell. Biol. 114, 1307.
      Ryan, M.C., and Sandell, L.J. 1990 J. Biol. Chem. 265, 10334.
      Mayne, R., and Burgeson, R.E. 1987 Structure and Function of Collagen Types,Academic Press, Orlando, FL
      Data Sheet

      Note that this data sheet is not lot-specific and is representative of the current specifications for this product. Please consult the vial label and the certificate of analysis for information on specific lots. Also note that shipping conditions may differ from storage conditions.

      Revision10-July-2007 JSW
      ApplicationFrozen Sections (1-10 µg/ml)
      Immunoblotting (1-5 µg/ml, see application references)
      Paraffin Sections (1-10 µg/ml, see application references)
      DescriptionPurified mouse monoclonal antibody generated by immunizing BALB/c mice with the specified immunogen and fusing splenocytes with P3-X-63-Ag8-LUI mouse myeloma cells. Recognizes the ~130 kDa type II collagen protein.
      BackgroundCollagens are the major fibrous structural elements of cartilage, tendon, skin, bone, lung and blood vessels. Their function is to hold cells together and thereby form discrete tissues. Collagens form insoluble fibers that have a high intrinsic tensile strength. Structurally, the collagens are composed of three polypeptide chains, αchains, which exist as three distinct subclasses each of which in turn is made up of numerous subtypes. Type II collagen is a major component of cartilage. It is translated as a pro-form which is then processed to yield the mature protein. Collagen II can also be expressed in two different forms, IIA and IIB, as a result of alternate splicing involving the inclusion of exon. The protein is synthesized primarily by chondrocytes and by certain cells in the eye, and exists as a trimer of α1(II) chains. Mutations in collagen II have been documented in chondrodysplasias and ostoarthrosis.
      HostMouse
      Immunogen speciesHuman
      Immunogenpurified collagen type II
      CloneII-4C11
      IsotypeIgG₁
      Speciesbovine, human, rabbit, rat
      FormLiquid
      FormulationIn 100 mM sodium phosphate buffer, 0.1% BSA, pH 7.0.
      Concentration Label Please refer to vial label for lot-specific concentration
      Preservative≤0.1% sodium azide
      CommentsMay react with other collagens. When used at high concentrations higher bands corresponding to aggregates may also be detected. This antibody may also react with other classes of collagen at a concentration higher than 1 µg/ml. In immunoblotting, a major band of ~130 kDa is obtained. Antibody should be titrated for optimal results in individual systems.
      Storage Avoid freeze/thaw
      -20°C
      Do Not Freeze Ok to freeze
      Special InstructionsFollowing initial thaw, aliquot and freeze (-20°C).
      Toxicity Standard Handling
      ReferencesKumagai, J. et al. 1994 J. Anat. 185, 278.
      Kivrikko, K,I. 1993 Ann. Med. 25, 113.
      Sandell, L.J., et al. 1991 J. Cell. Biol. 114, 1307.
      Ryan, M.C., and Sandell, L.J. 1990 J. Biol. Chem. 265, 10334.
      Mayne, R., and Burgeson, R.E. 1987 Structure and Function of Collagen Types,Academic Press, Orlando, FL
      Application referencesImmunoblotting Kumagai, J. et al. 1994 J. Anat. 185, 278. Paraffin Sections Kumagai, J. et al. 1994 J. Anat. 185, 278.