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MABF2119-100UG Anti-CD318 (CD6L) Antibody, clone 3A11

Anti-CD318 (CD6L), clone 3A11, Cat. No. MABF2119, is a mouse monoclonal antibody that detects CD318 (CD6L) and has been tested for use in Flow Cytometry, Immunocytochemistry, Immunohistochemistry (Paraffin), Immunoprecipitation, and Western Blotting.

Anti-CD318 (CD6L) Antibody, clone 3A11 MSDS (material safety data sheet) or SDS, CoA and CoQ, dossiers, brochures and other available documents.
Research Category: Inflammation & Immunology Gene Symbol: CDCP1, TRASK UniProt Number: Q9H5V8
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MABF2119-100UG
100 μg  
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      Overview

      Replacement Information

      Key Spec Table

      Species ReactivityKey ApplicationsHostFormatAntibody Type
      HFC, ICC, IH(P), IP, WBMPurifiedMonoclonal Antibody
      Description
      Catalogue NumberMABF2119-100UG
      DescriptionAnti-CD318 (CD6L) Antibody, clone 3A11
      Alternate Names
      • CUB domain-containing protein 1
      • Membrane glycoprotein gp140
      • Subtractive immunization M plus HEp3-associated 135 kDa protein
      • SIMA135
      • Transmembrane and associated with src kinases
      • CD6L
      Background InformationCUB domain-containing protein 1 (UniProt: Q9H5V8; also known as Membrane glycoprotein gp140, Subtractive immunization M plus HEp3-associated 135 kDa protein, SIMA135, Transmembrane and associated with Src kinases, CD318, CD6L) is encoded by the CDCP1 (also known as TRASK) gene (Gene ID:64866) in human. CD318 is a single-pass membrane protein (isoform 1) that serves as a ligand for CD6. It is highly expressed in mitotic cells and has low expression during interphase. It is detected in high levels in skeletal muscle and colon tissue and in lower levels in kidney, placenta, lungs, and small intestine. Its levels are shown to be up-regulated in several human cancer tissues. It is also expressed in high levels in synovial tissues where it participates in CD6-dependent adhesion of T cells to synovial fibroblasts. Presence of CD318 on cancer cells is associated with their aggressiveness and metastatic potential. Preincubation of cancer cells with clone 3A11 is shown to enhance killing of these cells and inhibits their survival. CD318 is shown to be chemoattractive to T cells and levels of soluble CD318 are increased in the synovial fluid from patients with rheumatoid arthritis and juvenile inflammatory arthritis. CD318 is synthesized with a signal peptide (aa 1-29), which is cleaved off in the mature form. It has an extracellular domain (aa 30-667), a transmembrane domain (aa 668-688), and a cytoplasmic domain (aa 689-836). Three isoforms of CD318 have been described that are produced by alternative splicing. (Ref.:Ruth, JH., et al. (2021). JCI Insight. doi: 10.1172/jci.insight.145662. Enyindah-Asonye, G., et al. (2017). Proc. Natl. Acad. Sci. USA 114(33); E6912-E6921).
      References
      Product Information
      FormatPurified
      PresentationPurified mouse monoclonal antibody IgG1 in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide.
      Applications
      ApplicationAnti-CD318 (CD6L), clone 3A11, Cat. No. MABF2119, is a mouse monoclonal antibody that detects CD318 (CD6L) and has been tested for use in Flow Cytometry, Immunocytochemistry, Immunohistochemistry (Paraffin), Immunoprecipitation, and Western Blotting.
      Key Applications
      • Flow Cytometry
      • Immunocytochemistry
      • Immunohistochemistry (Paraffin)
      • Immunoprecipitation
      • Western Blotting
      Application NotesImmunoprecipitation Analysis: A representative lot immunoprecipitated CD318 (CD6L) in Immunoprecipitation applications (Saifullah, M.K., et. al. (2004). J Immunol. 173(10):6125-33; Enyindah-Asonye, G., et. al. (2017). Proc Natl Acad Sci U S A. 114(33):E6912-E6921).

      Immunohistochemistry (Paraffin) Analysis: A representative lot detected CD318 (CD6L) in Immunohistochemistry applications (Enyindah-Asonye, G., et. al. (2017). Proc Natl Acad Sci U S A. 114(33):E6912-E6921).

      Western Blotting Analysis: A representative lot detected CD318 (CD6L) in Western Blotting applications (Saifullah, M.K., et. al. (2004). J Immunol. 173(10):6125-33; Enyindah-Asonye, G., et. al. (2017). Proc Natl Acad Sci U S A. 114(33):E6912-E6921).

      Flow Cytometry Analysis: A representative lot detected CD318 (CD6L) in Flow Cytometry applications (Saifullah, M.K., et. al. (2004). J Immunol. 173(10):6125-33; Enyindah-Asonye, G., et. al. (2017). Proc Natl Acad Sci U S A. 114(33):E6912-E6921).
      Biological Information
      ImmunogenHBL-100 human mammary gland epithelial cells.
      Epitopeextracellular domain
      Clone3A11
      ConcentrationPlease refer to lot specific datasheet.
      HostMouse
      SpecificityClone 3A11 specifically detects CD318 in human cells. It targets an epitope with in the extracellular domain.
      IsotypeIgG1κ
      Species Reactivity
      • Human
      Species Reactivity NoteHuman.
      Antibody TypeMonoclonal Antibody
      Entrez Gene Number
      Gene Symbol
      • CDCP1
      • TRASK
      Purification MethodProtein G purified
      UniProt Number
      Molecular Weight92.93 kDa calculated.
      Physicochemical Information
      Dimensions
      Materials Information
      Toxicological Information
      Safety Information according to GHS
      Safety Information
      Product Usage Statements
      Quality AssuranceEvaluated by Immunocytochemistry in HaCaT epidermal keratinocyte cells.

      Immunocytochemistry Analysis: A 1:1,000 dilution of this antibody detected CD318 (CD6L) in HaCaT epidermal keratinocyte cells.
      Usage Statement
      • Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
      Storage and Shipping Information
      Storage ConditionsStable for 1 year at 2-8°C from date of receipt.
      Packaging Information
      Material Size100 μg
      Transport Information
      Supplemental Information
      Specifications
      Global Trade Item Number
      Catalogue Number GTIN
      MABF2119-100UG 04054839573071

      Documentation

      Anti-CD318 (CD6L) Antibody, clone 3A11 SDS

      Title

      Safety Data Sheet (SDS) 

      Anti-CD318 (CD6L) Antibody, clone 3A11 Certificates of Analysis

      TitleLot Number
      Anti-CD318 (CD6L), clone 3A11 - 3863367 3863367
      Anti-CD318 (CD6L), clone 3A11 - 3941854 3941854
      Anti-CD318 (CD6L), clone 3A11 - 3975178 3975178
      Anti-CD318 (CD6L), clone 3A11 - 3987754 3987754
      Anti-CD318 (CD6L), clone 3A11 - 4010124 4010124
      Anti-CD318 (CD6L), clone 3A11 - 4123397 4123397
      Anti-CD318 (CD6L), clone 3A11 Monoclonal Antibody Q3108820