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MABS1247 Anti-BIG1 Antibody, clone 102

Detect BIG1 using this mouse monoclonal Anti-BIG1 Antibody, clone 102, Cat. No. MABS1247. It has been tested in Immunocytochemistry and Western Blotting.

Anti-BIG1 Antibody, clone 102 MSDS (material safety data sheet) or SDS, CoA and CoQ, dossiers, brochures and other available documents.
Research Category: Signaling Gene Symbol: ARFGEF1, ARFGEP1, BIG1 UniProt Number: Q9Y6D6
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MABS1247
100 μg  
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      Overview

      Replacement Information

      Key Spec Table

      Species ReactivityKey ApplicationsHostFormatAntibody Type
      HWB, ICCMPurifiedMonoclonal Antibody
      Description
      Catalogue NumberMABS1247
      DescriptionAnti-BIG1 Antibody, clone 102
      Alternate Names
      • Brefeldin A-inhibited guanine nucleotide-exchange protein 1
      • Brefeldin A-inhibited GEP 1
      • ADP-ribosylation factor guanine nucleotide-exchange factor 1
      • p200 ARF guanine nucleotide exchange factor
      • p200 ARF-GEP1
      Background InformationBrefeldin A-inhibited guanine nucleotide-exchange protein 1 (UniProt: Q9Y6D6; also known as BIG1, Brefeldin A-inhibited GEP 1, ADP-ribosylation factor guanine nucleotide-exchange factor 1, p200 ARF guanine nucleotide exchange factor, p200 ARF-GEP1) is encoded by the ARFGEF1 (also known as ARFGEP1, BIG1) gene (Gene ID: 10565) in human. BIG1 is expressed in placenta, lung, heart, brain, kidney and pancreas. BIG1 activates ADP-ribosylation factor (ARF) GTPases and is involved in vesicular trafficking. It promotes the activation of ARF1/ARF3 through replacement of GDP with GTP. BIG1 is required for the maintenance of Golgi structure and this function may be independent of its GEF activity. BIG1 and BIG2 display about 74% homology in their amino acid sequence and about 90% identity in their Sec7 domains. BIG1 is phosphorylated by PKA that reduces its GEF activity. It is localized in the cytoplasm in a multiprotein complex along with BIG2. Its nuclear localization has also been reported and it contains a nuclear localization sequence (aa 711-715). In serum starved cells treated with FK506, BIG1 is essentially completely concentrated in nuclei. BIG1 is shown to be a binding partner of myosin IXb and regulates its Rho-GTPase activating protein activity. Here BIG1 and RhoA compete with each other for the binding to myosin IXb, thus resulting in the inhibition of the GAP activity by BIG1. (Ref.: Lin, S et al. (2013). Arterioscler Thromb Vasc Biol. 33(2): e31-e38).
      References
      Product Information
      FormatPurified
      PresentationPurified mouse monoclonal antibody IgG1 in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide.
      Quality LevelMQ100
      Applications
      ApplicationDetect BIG1 using this mouse monoclonal Anti-BIG1 Antibody, clone 102, Cat. No. MABS1247. It has been tested in Immunocytochemistry and Western Blotting.
      Key Applications
      • Western Blotting
      • Immunocytochemistry
      Application NotesWestern Blotting Analysis: 0.5 µg/mL of this antibody detected BIG1 in 10 µg of A431 and HEK293 cell lysates.

      Immunocytochemistry Analysis: A representative lot detected BIG1 in Immunocytochemistry applications (Lowery, J., et. al. (2013). J Biol Chem. 288(16):11532-45).

      Western Blotting Analysis: A representative lot detected BIG1 in Western Blotting applications (Lowery, J., et. al. (2013). J Biol Chem. 288(16):11532-45).
      Biological Information
      ImmunogenGST-tagged recombinat fragment corresponding to 311 amino acids from human Brefeldin A-inhibited guanine nucleotide-exchange protein 1 (BIG1).
      Clone102
      ConcentrationPlease refer to lot specific datasheet.
      HostMouse
      SpecificityClone 102 specifically detects BIG1 protein in human and targets an epitope within amino acids 212-522 in the N-terminal region.
      IsotypeIgG1κ
      Species Reactivity
      • Human
      Species Reactivity NoteHuman.
      Antibody TypeMonoclonal Antibody
      Entrez Gene Number
      Gene Symbol
      • ARFGEF1
      • ARFGEP1
      • BIG1
      Purification MethodProtein G purified
      UniProt Number
      Molecular Weight~209 kDa observed; 208.77 kDa calculated. Uncharacterized bands may be observed in some lysate(s).
      Physicochemical Information
      Dimensions
      Materials Information
      Toxicological Information
      Safety Information according to GHS
      Safety Information
      Product Usage Statements
      Quality AssuranceEvaluated by Western Blotting in Jurkat cell lysate.

      Western Blotting Analysis: 0.5 µg/mL of this antibody detected BIG1 in 10 µg of Jurkat cell lysate.
      Usage Statement
      • Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
      Storage and Shipping Information
      Storage ConditionsStable for 1 year at 2-8°C from date of receipt.
      Packaging Information
      Material Size100 μg
      Transport Information
      Supplemental Information
      Specifications
      Global Trade Item Number
      Catalogue Number GTIN
      MABS1247 04054839174476