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113200 Anti-α-Actin Mouse mAb (1A4)

Overview

Key Spec Table

Species ReactivityHostAntibody Type
Bab, H, RbMMonoclonal Antibody

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113200-500UL
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      Description
      OverviewRecognizes α-actin in smooth muscle cells of blood vessels and parenchymal tissue of the intestine, testis, and ovary. Does not recognize actin from fibroblasts (β- and γ-cytoplasmic), striated muscle (α-sarcomeric), or myocardium (α-myocardial).
    • Antibody Target Gene Symbol: ACTA2
    • Target Synonym: 0610041G09RIK, a-SMA, AAT6, ACTSA, Actvs, ALPHA ACTIN VSM, ALPHA SMA, ALPHA SMOOTH MUSCLE ACTIN, ALPHA-ACTIN 2, FLJ45296, MGC127800, RGD: 621676, Similar to vascular alpha actin, Sm alpha actin, SMalphaA, SMOOTH MUSCLE ALPHA ACTIN
    • Entrez Gene Name: actin, alpha 2, smooth muscle, aorta
    • Hu Entrez ID: 59 (Related Antibodies: 113200)
    • Rat Entrez ID: 81633
    • Catalogue Number113200
      Brand Family Calbiochem®
      References
      ReferencesRannov-Jennsen, L., et al. 1990. Lab Invest. 63, 532.
      Skalli, O., et al. 1989. J. Histochem. Cytochem 37, 315.
      Skalli, O., et al. 1986. J. Cell. Biol. 103, 2787.
      Product Information
      FormLiquid
      FormulationIn 150 mM NaCl, 20 mM Tris-borate, BHC-6 carrier polysaccharides, pH 7.5.
      Negative controlFibroblasts, striated muscle, or myocardium
      Positive controlSmooth muscle cells of blood vessels and parenchymal tissue of intestine, testis, or ovary
      Preservative≤0.1% sodium azide
      Quality LevelMQ100
      Applications
      Key Applications Frozen Sections
      Paraffin Sections
      Application NotesFrozen Sections (1:50-1:75)
      Immunoblotting (see comments)
      Paraffin Sections (1:50-1:75)
      Application CommentsDoes not recognize actin from fibroblasts (β- and γ-cytoplasmic), striated muscle (α-sarcomeric), or myocardium (α-myocardial).Prolonged fixation in buffered formalin destroys the epitope. The antibody is specific for the α-smooth muscle isoform of actin. This antibody is also reported to work for immunoblotting. Variables associated with assay conditions will dictate the proper working dilution.
      Biological Information
      Immunogena synthetic peptide corresponding to amino acids 1-10 of human smooth muscle α-actin.
      ImmunogenHuman Smooth Muscle
      Clone1A4
      HostMouse
      IsotypeIgG2a
      Species Reactivity
      • Baboon
      • Human
      • Rabbit
      Antibody TypeMonoclonal Antibody
      Concentration Label Please refer to vial label for lot-specific concentration
      Storage and Shipping Information
      Ship Code Blue Ice Only
      Toxicity Standard Handling
      Storage +2°C to +8°C
      Do not freeze Yes
      Global Trade Item Number
      Catalogue Number GTIN
      113200-500UL 04055977208054