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MABF3141-100UG Anti-ANTXR2/CMG-2 Antibody, clone 1H8

Anti-ANTXR2/CMG-2, clone 1H8, Cat. No. MABF3141, is a mouse monoclonal antibody that detects ANTXR2/CMG-2 and is tested for use in Western Blotting.

MABF3141-100UG
100 μg  
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      Overview

      Replacement Information
      Description
      Catalogue NumberMABF3141-100UG
      DescriptionAnti-ANTXR2/CMG-2 Antibody, clone 1H8
      Alternate Names
      • Anthrax toxin receptor 2
      • Capillary morphogenesis gene 2vprotein
      • CMG-2
      Background InformationAnthrax toxin receptor 2 (UniProt: P58335; also known as Capillary morphogenesis gene 2 protein, CMG-2) is encoded by the ANTRX2 (also known as CMG2) gene (Gene ID: 118429) in human. ANTRX2/CMG-2 is widely expressed, single-pass type I membrane protein that is synthesized with a signal peptide (aa 1-33), which is subsequently cleaved off to produce the mature form that contains an extracellular domain (aa 34-318), a transmembrane domain (aa 319-341), and a cytoplasmic domain (aa 342-489). It shares about 58% amino acid identity with ANTXR1. Four isoforms of ANTRX2/CMG-2 have been described that are produced by alternative splicing. It is reported to be essential for cellular interactions with laminin and the extracellular matrix. It is an important regulator of collagen VI homeostasis. It mediates the transport of collagen to lysosomes for degradation. Its loss promotes accumulation of Collagen VI leading to nodule formation. Antrx2 -/- mice are shown to display massive accumulation of collagen VI in their uteri leading to fibrosis and sterility. It binds to collagen VI via its vWA domain (aa 44-213) in a MIDAS (metal ion-dependent binding domain) manner. (Ref.: Burgi, J., et al. (2017). Nat. Commun. 8; Article 15861; Chaudhary, A., et al. (2012). Cancer Cell. 21(2): 212-226).
      References
      Product Information
      FormatPurified
      PresentationPurified mouse monoclonal antibody IgG2c in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide.
      Quality LevelMQ200
      Applications
      ApplicationAnti-ANTXR2/CMG-2, clone 1H8, Cat. No. MABF3141, is a mouse monoclonal antibody that detects ANTXR2/CMG-2 and is tested for use in Western Blotting.
      Key Applications
      • Western Blotting
      Application NotesTested applications

      Western Blotting Analysis: A representative lot detected ANTXR2/CMG-2 in Western Blotting applications (Chaudhary, A., et al. (2012). Cancer Cell. 21(2):212-26; Szot, C., et al. (2018). J Clin Invest. 128(7):2927-2943).

      Note: Actual optimal working dilutions must be determined by end user as specimens, and experimental conditions may vary with the end user.
      Biological Information
      ImmunogenA recombinant fragment of 178 amino acids from the N-terminal half of human ANTXR2 with an N-terminal extension of GSHMLEDPRG.
      EpitopeN-terminal half
      Clone1H8
      Concentration1.0 mg/mL. Please refer to guidance on suggested starting dilutions and/or titers per application and sample type.
      HostMouse
      SpecificityClone 1H8 is a mouse monoclonal antibody that detects Anthrax toxin receptor 2 (ANTXR2). It targets an epitope within the N-terminal half.
      IsotypeIgG2cκ
      Species Reactivity
      • Mouse
      • Human
      Species Reactivity NoteHuman, Mouse.
      Antibody TypeMonoclonal Antibody
      Entrez Gene Number
      Gene Symbol
      • ANTXR2
      Purification MethodProtein G purified
      UniProt Number
      Molecular Weight~54 kDa observed; 53.67 kDa calculated. Uncharacterized bands may be observed in some lysate(s).
      Physicochemical Information
      Dimensions
      Materials Information
      Toxicological Information
      Safety Information according to GHS
      Safety Information
      Product Usage Statements
      Quality AssuranceEvaluated by Western Blotting in Mouse lung tissue lysate.

      Western Blotting Analysis: A 1:1,000 dilution of this antibody detected ANTXR2/CMG-2 in Mouse lung tissue lysate.
      Usage Statement
      • Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
      Storage and Shipping Information
      Storage ConditionsRecommended storage: +2°C to +8°C.
      Packaging Information
      Material Size100 μg
      Transport Information
      Supplemental Information
      Specifications
      Global Trade Item Number
      Catalogue Number GTIN
      MABF3141-100UG 04065270172532