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MABN2653-25UG Anti-3-Hydroxykynurenine Antibody, clone 2c4-B6-C2

Anti-3-Hydroxykynurenine, clone 2c4-B6-C2, Cat. No. MABN2653, is a mouse monoclonal antibody that detects 3-hydroxykynurenine (3OHKYN) and is tested for use in ELISA, Immunocytochemistry, Immunohistochemistry, and Western Blotting.

MABN2653-25UG
25 μg  
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      Overview

      Replacement Information
      Description
      Catalogue NumberMABN2653-25UG
      DescriptionAnti-3-Hydroxykynurenine Antibody, clone 2c4-B6-C2
      Alternate Names
      • 3-Hydroxy-DL-kynurenine
      Background InformationKynurenine, a breakdown product of tryptophan, is produced by the action of tryptophan dioxygenase mainly in the liver. Kynurenine and its breakdown products perform several biological functions, including dilating blood vessels during inflammation and regulating the immune response. The kynurenine pathway is reported to be involved in the synthesis of NAD+ from the L-tryptophan and alterations in this pathway have been linked to several neurological and psychiatric disorders. Kynurenine and its metabolites can readily pass-through cell membranes and can diffuse through the cortex of the lens. Kynurenine plays a protective role in the eye by shielding the retina from UV damage. However, it can also cause damage to lens proteins if glutathione levels are not sufficient to prevent the reaction of kynurenines with proteins. Kynurenines undergo spontaneous deamination to form ,β-unsaturated ketones that are highly reactive with nucleophilic amino acids in lens proteins. Kynurenine levels decrease with age and with cataract formation, which is mainly via its reaction with lens proteins. Clone 2c4-B6-C2 recognizes kynurenine-derived structure containing a hydroxyl group on benzene ring. It reacts with 3-hydrxy kynurenine N -acetyl lysine, N -acetyl histidine, N acetyl arginine, and N acetyl cysteine. It is requires 3-OH and 2-NH2 groups in the benzene ring to recognize the antigen. (Ref.: Mailankot, M, and Nagaraj, RH. (2010). Int. J. Biochem. Cell Biol. 42(9); 1446-1454; Staniszewska, MM., and Nagaraj, RH. (2005). J. Biol. Chem. 280(23); 22154-22164).
      References
      Product Information
      FormatPurified
      PresentationPurified mouse monoclonal antibody IgG1 in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide.
      Quality LevelMQ200
      Applications
      ApplicationAnti-3-Hydroxykynurenine, clone 2c4-B6-C2, Cat. No. MABN2653, is a mouse monoclonal antibody that detects 3-hydroxykynurenine (3OHKYN) and is tested for use in ELISA, Immunocytochemistry, Immunohistochemistry, and Western Blotting.
      Key Applications
      • Western Blotting
      • Immunohistochemistry
      • Immunocytochemistry
      • ELISA
      Application NotesTested applications

      ELISA Analysis: A representative lot detected 3-Hydroxykynurenine in ELISA applications (Staniszewska, M.M., et al. (2005). J Biol Chem. 280(23); 21154-64).

      Western Blotting Analysis: A representative lot detected 3-Hydroxykynurenine in Western Blotting applications (Staniszewska, M.M., et al. (2005). J Biol Chem. 280(23);21154-64).

      Immunohistochemistry Applications: A representative lot detected 3-Hydroxykynurenine in Immunohistochemistry applications (Staniszewska, M.M., et al. (2005). J Biol Chem. 280(23); 21154-64).

      Immunocytochemistry Analysis: A representative lot detected 3-Hydroxykynurenine in Immunocytochemistry applications (Mailankot, M., et al. (2010). Int J Biochem Cell Biol. 42(9); 1446-54).

      Note: Actual optimal working dilutions must be determined by end user as specimens, and experimental conditions may vary with the end user.
      Biological Information
      ImmunogenKLH-conjugated 3-Hydroxykynurenine.
      EpitopeUnknown
      Clone2c4-B6-C2
      Concentration1.0 mg/mL. Please refer to guidance on suggested starting dilutions and/or titers per application and sample type.
      HostMouse
      SpecificityClone 2c4-B6-C2 is a mouse monoclonal antibody that detects 3-Hydroxykynurenine.
      IsotypeIgG1κ
      Species Reactivity
      • Human
      Species Reactivity NoteHuman. Predicted to react with all mammals speices.
      Antibody TypeMonoclonal Antibody
      Entrez Gene Number
      Purification MethodProtein G purified
      UniProt Number
      Molecular Weight~60 kDa observed for BSA-conjugated 3-Hydroxykynurenine. 224.21 Da calculated for 3-Hydroxykynurenine. Uncharacterized bands may be observed in some lysate(s).
      Physicochemical Information
      Dimensions
      Materials Information
      Toxicological Information
      Safety Information according to GHS
      Safety Information
      Product Usage Statements
      Quality AssuranceEvaluated by Western Blotting with 3-hydoxykynurenine modified bovine serum albumin (BSA).

      Western Blotting Analysis (WB): A 1:1,000 dilution of this antibody detected 3-hydroxykynurenine (3-OHKYN) modified BSA , but not in unmodified BSA.
      Usage Statement
      • Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
      Storage and Shipping Information
      Storage ConditionsRecommended storage: +2°C to +8°C.
      Packaging Information
      Material Size25 μg
      Transport Information
      Supplemental Information
      Specifications
      Global Trade Item Number
      Catalogue Number GTIN
      MABN2653-25UG 04065269802471