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71234 pBiEx™-1 DNA - Novagen

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      Description
      OverviewThe pBiEx™ vectors are designed for cloning and high-level expression of proteins in both bacteria and in transiently transfected Spodoptera-derived insect cells. In E. coli, target gene transcription is regulated by the tightly controlled T7lac promoter, and a ribosome binding site (rbs) directs translation initiation. In insect cells, transcription is driven by the AcNPV-derived hr5 enhancer and immediate early promoter, IE1.

      pBiEx-1 contains N-terminal His•Tag and S•Tag coding sequences, and a C-terminal HSV•Tag coding sequence. For sequencing or PCR of recombinants in pBiEx-1, refer to the table below to choose the appropriate primers.






      This product is sold for internal research use only. Any commercial use of this product, its components, and/or any derivatives thereof (including but not limited to proteins produced using the product or its components) (together and hereinafter the 'EMD Product') requires signature of a written commercial use agreement with EMD Millipore Corporation or its successor-in-interest. Commercial use shall include but not be limited to: (1) use of the EMD Product to manufacture products for sale to third parties; (2) use of the EMD Product to provide services, information, or data to third parties in exchange for consideration; (3) use of the EMD Product for therapeutic, diagnostic or prophylactic purposes (including as part of a device, chip, assay or other product); or (4) resale of the EMD Product, whether or not such EMD Product is resold for research use. Nothing contained herein shall be deemed to represent or warrant that additional third party rights are not required for use of the EMD Product. Please direct any questions on these use restrictions to: licensing@milliporesigma.com.
      Vector5' Primer3' Primeralt 5' Primer
      pIEx-1S•Tag 18mer PrimerAS HSV•Tag PrimerIE1 Promoter Primer
      pIEx-2S•Tag 18mer PrimerAS HSV•Tag PrimerIE1 Promoter Primer
      pIEx-3S•Tag 18mer PrimerAS HSV•Tag PrimerIE1 Promoter Primer
      pIEx-4IE1 Promoter PrimerAS S•Tag 18mer Primer
      NA
      pIEx-5IE1 Promoter PrimerAS S•Tag 18mer Primer
      NA
      pBiEx-1S•Tag 18mer PrimerAS HSV•Tag PrimerIE1 Promoter Primer
      T7 Promoter Primer
      pBiEx-2S•Tag 18mer PrimerAS HSV•Tag PrimerIE1 Promoter Primer
      T7 Promoter Primer
      pBiEx-3IE1 Promoter PrimerAS S•Tag 18mer PrimerT7 Promoter Primer
      Catalogue Number71234
      Brand Family Novagen®
      SynonymsInsectDirect™ System- pBiEx™-1 DNA
      References
      Product Information
      Quality LevelMQ100
      Applications
      Biological Information
      Physicochemical Information
      Dimensions
      Materials Information
      Toxicological Information
      Safety Information according to GHS
      Safety Information
      Product Usage Statements
      Storage and Shipping Information
      Ship Code Shipped with Blue Ice or with Dry Ice
      Toxicity Standard Handling
      Storage -20°C
      Do not freeze Ok to freeze
      Packaging Information
      Transport Information
      Supplemental Information
      Specifications
      Global Trade Item Number
      Número de referencia GTIN
      71234-3 04055977271232

      Documentation

      pBiEx™-1 DNA - Novagen Ficha datos de seguridad (MSDS)

      Título

      Ficha técnica de seguridad del material (MSDS) 

      pBiEx™-1 DNA - Novagen Certificados de análisis

      CargoNúmero de lote
      71234

      Folleto

      Cargo
      Insect Cell Expression
      Insect Directル Protein Expression & Purification System

      Protocolos de usuario

      Cargo
      TB053 Academic and Non-profit Laboratory Assurance Letter
      TB357 pBiEx Vectors

      Mapa vectorial

      Cargo
      TB354 pBiEx™-1 Vector Map

      Secuencia vectorial

      Cargo
      pBiEx™-1 Sequence