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WB59 Western-Re-Probe™ Reagent

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WB59
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WB59-100ML
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      Description
      OverviewDesigned to dissociate and remove antibodies from membrane-bound proteins without destroying antigenic binding capacity. Recommended for stripping chemiluminescent or radioisotopic signals from blots. Not recommended for stripping color-producing Western blots. Supplied as a 5X solution sufficient for stripping 25-30 standard blots.
      A special formulation designed to dissociate and remove antibodies from the membrane bound protein without destroying the antigenic binding affinity.
      Catalogue NumberWB59
      Brand Family Calbiochem®
      Product Information
      FormLiquid
      FormulationSupplied as a 5X solution.
      Kit contains5X Re-Probe solution (125 ml)
      Quality LevelMQ100
      Applications
      Key Applications Immunoblotting (Western Blotting)
      Application NotesImmunoblotting (see comments)
      Application CommentsSufficient volume to strip 25-30 standard blots.

      ADVANTAGES
      -Immunoblotting analyzed repeatedly with multiple probes
      -Saving on hard-to-get reagents and samples
      -Correction and re-analysis of unsatisfactory Western Blots
      -Saving time spent in gel re-running and transfer processes

      PROTOCOL
      1- Keep the membrane wet in an appropriate buffer after each probing procedure. Prepare 20-25 ml solution of 1X Western-Re-Probe™ in water.
      2- Incubate the membrane in 1X Western-Re-Probe™ buffer for 30 min at room temperature (RT) with shaking.
      3- Wash the membrane three times in PBST or TBST buffer containing 0.1% Tween®-20 detergent. Use a large volume of washing buffer in a tray for the washing step. Each wash cycle involves agitating the membrane 5 min in the washing buffer.
      4- Block the membrane and perform immunodetection.

      Western-Re-Probe™ Reagent can be used for stripping and re-probing membranes 2-3 times. Some loss of signal may be noticed after second cycle of stripping and re-probing.

      PROTOCOL SUMMARY
      1. Incubate membrane in 1X Western-Re-Probe™ (30 min at RT) with shaking
      2. Wash the membrane 3 times in PBST or TBST buffer
      3. Block membrane and perform immunodetection
      Storage and Shipping Information
      Ship Code Blue Ice Only
      Toxicity Standard Handling
      Storage +2°C to +8°C
      Do not freeze Ok to freeze
      Supplemental Information
      Kit contains5X Re-Probe solution (125 ml)
      Global Trade Item Number
      Número de referencia GTIN
      WB59-100ML 04055977206746