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71351 Rosetta-gami™ 2(DE3) Competent Cells * - Novagen

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71351
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          Description
          OverviewRosetta-gami 2 host strains combine the advantages of Rosetta 2 and Origami 2 strains to alleviate codon bias and enhance disulfide bond formation in the cytoplasm when heterologoous proteins are expressed in E. coli. These trxB/gor mutants are compatible with kanamycin-resistant vectors, and carry the chloramphenicol-resistant pRARE2 plasmid, which supplies seven rare tRNAs.

          DE3 indicates that the host is a lysogen of λDE3, and therefore carries a chromosomal copy of the T7 RNA polymerase gene under control of the lacUV5 promoter. Such strains are suitable for production of protein from target genes cloned in pET vectors by induction with IPTG.
          Genotype: Δ(ara-leu)7697 ΔlacX74 ΔphoA PvuII phoR araD139 ahpC galE galK rpsL (DE3) F′[lac+ lacIq pro] gor522::Tn10 trxB pRARE2 (CamR, StrR, TetR)


          This product contains genetically modified organisms (GMO). Within the EU GMOs are regulated by Directives 2001/18/EC and 2009/41/EC of the European Parliament and of the Council and their national implementation in the member States respectively. This legislation obliges MilliporeSigma to request certain information about you and the establishment where the GMOs are being handled. Click here for Enduser Declaration (EUD) Form.




          This product is sold for internal research use only. Any commercial use of this product, its components, and/or any derivatives thereof (including but not limited to proteins produced using the product or its components) (together and hereinafter the 'EMD Product') requires signature of a written commercial use agreement with EMD Millipore Corporation or its successor-in-interest. Commercial use shall include but not be limited to: (1) use of the EMD Product to manufacture products for sale to third parties; (2) use of the EMD Product to provide services, information, or data to third parties in exchange for consideration; (3) use of the EMD Product for therapeutic, diagnostic or prophylactic purposes (including as part of a device, chip, assay or other product); or (4) resale of the EMD Product, whether or not such EMD Product is resold for research use. Nothing contained herein shall be deemed to represent or warrant that additional third party rights are not required for use of the EMD Product. Please direct any questions on these use restrictions to: licensing@milliporesigma.com.
          Catalogue Number71351
          Brand Family Novagen®
          References
          Product Information
          0.4 ml1 mlComponent
          2 × 0.2 ml5 × 0.2 mlRosetta-gami 2(DE3) Competent Cells
          2 × 2 ml4 × 2 mlSOC Medium
          10 µl10 µlTest Plasmid
          Guaranteed efficiency2 × 10⁶ cfu/µg
          Quality LevelMQ100
          Applications
          Biological Information
          Physicochemical Information
          Dimensions
          Materials Information
          Toxicological Information
          Safety Information according to GHS
          Safety Information
          Product Usage Statements
          Storage and Shipping Information
          Ship Code Dry Ice Only
          Toxicity Multiple Toxicity Values, refer to MSDS
          Storage ≤ -70°C
          Do not freeze Ok to freeze
          Packaging Information
          Transport Information
          Supplemental Information
          Specifications
          Global Trade Item Number
          Número de referencia GTIN
          71351-3 04055977253375
          71351-4 04055977253382

          Documentation

          Licencias necesarias

          Título
          CARTA DE USO GENERAL 2016
          PRODUCTO REGULADO POR LA SAGARPA
          PRODUCTO REGULADO POR LA SECRETARÍA DE SALUD

          Rosetta-gami™ 2(DE3) Competent Cells * - Novagen Ficha datos de seguridad (MSDS)

          Título

          Ficha técnica de seguridad del material (MSDS) 

          Rosetta-gami™ 2(DE3) Competent Cells * - Novagen Certificados de análisis

          CargoNúmero de lote
          71351

          Folleto

          Cargo
          Competent Cell Brochure

          Citas

          Título
        • Myong-Ok Park, Taeko Mizutani and Patrik R. Jones. (2007) Glyceraldehyde-3-phosphate ferredoxin oxidoreductase from Methanococcus maripaludis. Journal of Bacteriology 189, 7281-7289.
        • Sabine Oertelt, et al. (2006) Anti-mitochondrial antibodies and primary biliary cirrhosis in TGF-beta receptor II dominant-negative mice. Journal of Immunology 177, 1655-1660.
        • Protocolos de usuario

          Cargo
          TB009 Competent Cells
          TB053 Academic and Non-profit Laboratory Assurance Letter
          TB055 pET System Manual

          Productos y aplicaciones relacionados

          Related Products

          Número de referencia Descripción  
          71350 Rosetta-gami™ 2 Competent Cells - Novagen Precios y disponibilidad
          71352 Rosetta-gami™ 2(DE3)pLysS Competent Cells - Novagen Precios y disponibilidad
          71432 Rosetta-gami™ 2 Competent Cell Set - Novagen Precios y disponibilidad

          Productos relacionados por: Brand Facete

          Categorías

          Life Science Research > Genomic Analysis > Transfection and Protein Expression > Bacterial Expression > Competent Cells for Expression