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71092 PopCulture® Reagent

PopCulture® Reagent: Ficha de datos de seguridad (MSDS o SDS), certificado de análisis y de calidad (CoA y CoQ), expedientes, folletos y otros documentos disponibles.
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71092
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Replacement Information

Precios y disponibilidad

Número de referencia DisponiblidadEmbalaje Cant./Env. Precio Cantidad
71092-3
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      71092-5
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          Description
          OverviewPopCulture Reagent* is a buffered mixture of concentrated detergents formulated to extract proteins from E. coli cells directly in their culture medium. Using this method, cell culture, protein extraction and purification performed in the original culture tube or multiwell plate. PopCulture perforates the E. coli cell wall without denaturing soluble protein and protects protein from the pH extremes produced in high density culture media. Recombinant proteins can be assayed directly or purified by adding an affinity matrix, washing the matrix:target protein complex to remove culture medium and cellular contaminants and eluting the purified protein from the matrix. Purification of fusion proteins from total culture extracts has been demonstrated using both IMAC and GST affinity chromatography methods (1).

          To further enhance the PopCulture purification procedure, lysozyme and/or Benzonase® Nuclease may be added. Lysozyme cleaves a peptidoglycan bond in the E. coli cell wall, enhancing cell lysis and increasing the yield of protein (1, 2). Proteins may be expressed in a host encoding T7 lysozyme (pLysS host) or exogenous lysozyme may be added after the PopCulture Reagent. Benzonase Nuclease may also be added to degrade endogenous nucleic acids that may interfere with purification due to high viscosity.

          The PopCulture protein purification procedure is ideally suited to high throughput (HT) robotic processing of samples for proteomics research or any application that would benefit from the increased speed and convenience. The magnetic agarose capture resins (e.g. GST•Bind™ Magnetic Agarose Beads, His•Bind® Magnetic Agarose Beads) are optimal for HT applications since the entire procedure can be performed in a single tube without the need for columns or centrifugation.

          *patent pending
          Catalogue Number71092
          Brand Family Novagen®
          References
          References1. Grabski, A., Drott, D., Handley, M., Mehler, M. and Novy, R. (2001). inNovations 13, 1–4.

          2. Inouye, M., Arnheim, N. and Sternglanz, T. (1973). J. Biol. Chem. 248, 7247.
          Product Information
          DeclarationThis product is covered by EP Patent 1,432,822 owned by EMD Chemicals Inc. or its Affiliates.
          Quality LevelMQ100
          Applications
          Biological Information
          Physicochemical Information
          Dimensions
          Materials Information
          Toxicological Information
          Safety Information according to GHS
          Safety Information
          Product Usage Statements
          Storage and Shipping Information
          Ship Code Shipped at Ambient Temperature or with Blue Ice
          Toxicity Irritant
          Storage +15°C to +30°C
          Do not freeze Ok to freeze
          Packaging Information
          Transport Information
          Supplemental Information
          Specifications
          Global Trade Item Number
          Número de referencia GTIN
          71092-3 04055977271799
          71092-5 04055977271829

          Documentation

          PopCulture® Reagent Ficha datos de seguridad (MSDS)

          Título

          Ficha técnica de seguridad del material (MSDS) 

          PopCulture® Reagent Certificados de análisis

          CargoNúmero de lote
          71092

          Referencias bibliográficas

          Visión general referencias
          1. Grabski, A., Drott, D., Handley, M., Mehler, M. and Novy, R. (2001). inNovations 13, 1–4.

          2. Inouye, M., Arnheim, N. and Sternglanz, T. (1973). J. Biol. Chem. 248, 7247.

          Folleto

          Cargo
          Bulk Product Guide
          Overnight Express Autoinduction Systems
          Protein Purification and Detection Tools

          Citas

          Título
        • Andrew T. Anfora and Rodney A. Welch. (2006) DsdX is the second D-serine transporter in uropathogenic Escherichia coli clinical isolate CFT073. Journal of Bacteriology 188, 6622-6628.
        • Ilya G. Serebriiskii, et al. (2005) A combined yeast/bacterial two-hybrid system: development and evaluation. Molecular and Cellular Proteomics 4, 819-826.
        • Henry Nguyen, et al. (2004) An automated small-scale protein expression and purification screening provides beneficial information for protein production. Journal of Structural and Functional Genomics 5, 23-27.
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          Protocolos de usuario

          Cargo
          TB323 PopCulture® Reagent