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14-484 PKCα Protein, active, 10 µg

Active, recombinant, full-length human PKCα, containing N-terminal His6 tag. For use in Kinase Assays.

PKCα Protein, active, 10 µg: Ficha de datos de seguridad (MSDS o SDS), certificado de análisis y de calidad (CoA y CoQ), expedientes, folletos y otros documentos disponibles.
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14-484
10 µg  Also available in 250 µg size --call for pricing and availability and reference catalog number 14-484M when ordering the 250 µg size.
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      Descripción

      Replacement Information
      Description
      Catalogue Number14-484
      Brand Family Upstate
      Trade Name
      • Upstate
      DescriptionPKCα Protein, active, 10 µg
      OverviewRecombinant, full-length human PKCα, containing N-terminal His6 tag

      PKC Lipid activator (Catalog # 20-133) is required for use.
      References
      Product Information
      Quality LevelMQ100
      Applications
      ApplicationActive, recombinant, full-length human PKCα, containing N-terminal His6 tag. For use in Kinase Assays.
      Key Applications
      • Kinase Assay
      Biological Information
      SourceExpressed by baculovirus in Sf21 cells
      Specific ActivityFor Specific Activity data, refer to the Certificate of Analysis for individual lots of this enzyme.
      Entrez Gene Number
      Entrez Gene SummaryProtein kinase C (PKC) is a family of serine- and threonine-specific protein kinases that can be activated by calcium and the second messenger diacylglycerol. PKC family members phosphorylate a wide variety of protein targets and are known to be involved in diverse cellular signaling pathways. PKC family members also serve as major receptors for phorbol esters, a class of tumor promoters. Each member of the PKC family has a specific expression profile and is believed to play a distinct role in cells. The protein encoded by this gene is one of the PKC family members. This kinase has been reported to play roles in many different cellular processes, such as cell adhesion, cell transformation, cell cycle checkpoint, and cell volume control. Knockout studies in mice suggest that this kinase may be a fundamental regulator of cardiac contractility and Ca(2+) handling in myocytes.
      Gene Symbol
      • PRKCA
      • PKC-A
      • PKCA
      • MGC129901
      • AAG6
      • MGC129900
      • PRKACA
      • PKC-alpha
      Protein TargetPKCα
      Purification MethodNi2+/NTA-agarose
      Target Sub-FamilyAGC
      UniProt Number
      UniProt SummaryFUNCTION: SwissProt: P17252 # PKC is activated by diacylglycerol which in turn phosphorylates a range of cellular proteins. PKC also serves as the receptor for phorbol esters, a class of tumor promoters.
      COFACTOR: Binds 3 calcium ions per subunit. The ions are bound to the C2 domain (By similarity).
      SIZE: 672 amino acids; 76764 Da
      SUBUNIT: Interacts with CENTA1, CSPG4 and PRKCABP. Binds to SDPR in the presence of phosphatidylserine.
      SIMILARITY: SwissProt: P17252 ## Belongs to the protein kinase superfamily. AGC Ser/Thr protein kinase family. PKC subfamily. & Contains 1 AGC-kinase C-terminal domain. & Contains 1 C2 domain. & Contains 2 phorbol-ester/DAG-type zinc fingers. & Contains 1 protein kinase domain.
      Molecular Weight78kDa
      Physicochemical Information
      Dimensions
      Materials Information
      Toxicological Information
      Safety Information according to GHS
      Safety Information
      Product Usage Statements
      Quality Assuranceroutinely evaluated by phosphorylation of Histone H1.
      Usage Statement
      • Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
      Storage and Shipping Information
      Storage Conditions1 year at -70°C
      Packaging Information
      Material Size10 µg
      Material PackageAlso available in 250 µg size --call for pricing and availability and reference catalog number 14-484M when ordering the 250 µg size.
      Transport Information
      Supplemental Information
      Specifications
      Global Trade Item Number
      Número de referencia GTIN
      14-484 04053252329326

      Documentation

      PKCα Protein, active, 10 µg Ficha datos de seguridad (MSDS)

      Título

      Ficha técnica de seguridad del material (MSDS) 

      PKCα Protein, active, 10 µg Certificados de análisis

      CargoNúmero de lote
      PKC#945;, active - 33245U-M 33245U-M
      PKC#945;, active - 33245U-N 33245U-N
      PKC#945;, active - 33245U-P 33245U-P

      Preguntas frecuentes

      PreguntaRespuesta
      Why is it important to shake the reaction mixture during the incubations?It is important to shake the reaction mixture during incubations so that the substrate and the enzyme get good interaction. If you do not have access to a shaking incubator, use a water bath for the incubations, removing the tubes regularly to vortex.