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HPRTKMAG-01K MILLIPLEX MAP RTK Phosphoprotein Magnetic Bead Panel- Cell Signaling Multiplex Assay

Intracellular Bead-Based Multiplex Assays using the Luminex technology enables the simultaneous relative quantitation of multiple phosphorylation and total pathway proteins in tissue and cell lysate samples. Compare Multiplexing results to those of Western blotting.

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Key Applications: Multiplexing Detection Methods: Luminex xMAP Research Category: Signaling, Apoptosis & Cancer
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HPRTKMAG-01K
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Descripción

Replacement Information

Tabla espec. clave

Analytes AvailableSpecies ReactivityKey ApplicationsDetection Methods
c-Kit c-Met/HGFR (panTyr) EGFR (pan Tyr) ErbB2/HER2 ErbB3/HER3 (panTyr) ErbB4/HER4 (panTyr) FLT3 (pan Tyr) IGF-1R (pan Tyr) M-CSFR (pan Tyr) PDGFR-α (pan Tyr) PDGFRß (pan Tyr) TIE1 (pan Tyr) Tie2 (pan Tyr) VEGFR1 (pan Tyr) VEGFR2 (pan Tyr) VEGFR3 (pan Tyr) FGFR1 HMplexLuminex xMAP
Description
Catalogue NumberHPRTKMAG-01K
Trade Name
  • MILLIPLEX
DescriptionMILLIPLEX MAP RTK Phosphoprotein Magnetic Bead Panel- Cell Signaling Multiplex Assay
OverviewThe MILLIPLEX® MAP RTK Phosphoprotein Magnetic Bead Customizable Panel is used to detect changes in phosphorylated (panTyr) c-Kit, c-Met/HGFR, EGFR, ErbB2/HER2, ErbB3/HER3 , ErbB4/HER4 , FGFR1 , FLT3 , IGF-1R, M-CSFR, PDGFRα, PDGFRβ, TIE1, TIE2, VEGFR1, VEGFR2, and VEGFR3 in cell lysates using the Luminex® system.

MILLIPLEX® MAP offers the broadest selection of analytes across a wide range of pathways. Once the panel of interest has been identified, you can rely on the quality we build into each kit to produce results you can trust. Performance criteria evaluated during the validation process include: cross-reactivity, assay CVs, kit stability, and sample behavior. In addition each kit meets stringent Quality Control criteria to ensure lot-to-lot reproducibility.

The MILLIPLEX® MAP RTK Phosphoprotein Magnetic Bead Panel includes:
• Positive and negative control cell lysates provided to qualify assay performance
• Configurable magnetic beads to capture analytes of interest
• Optimized detection antibody cocktail designed to yield consistent analyte profiles within a panel
Background InformationRTKs (Receptor Tyrosine Kinases) are transmembrane proteins that act as receptors for growth factors, neurotrophic factors and other extracellular signaling molecules. Upon binding extracellular ligands, RTKs can undergo receptor dimerization and tyrosine phosphorylation on specific residues in the cytoplasmic tails which then lead to RTK-mediated signal transduction. Of the greater than 500 known protein kinases in human genome, approximately 60 are members of the RTK family. They are central components of cell signaling networks and play crucial roles in normal physiological processes and disease processes ranging from diabetes to cancer.
References
Product Information
Detection methodLuminex xMAP
ConfigurationDesign your multiplex kit by choosing available analytes within this panel.
Panel TypeCell Signaling
Quality LevelMQ200
Applications
Application Intracellular Bead-Based Multiplex Assays using the Luminex technology enables the simultaneous relative quantitation of multiple phosphorylation and total pathway proteins in tissue and cell lysate samples. Compare Multiplexing results to those of Western blotting.
Key Applications
  • Multiplexing
Application Notes• An overnight (4°C) incubation is recommended for best results.
• This assay requires 25 µL diluted cell lysate per well.
• This kit must be run using Assay Buffer 1 (provided).
• 1 - 25 μg cell lysate/well (recommended starting concentration is 40 to 1,000 μg protein/mL).
• This 17 plex panel , unlike most signaling kits that are fixed/premixed, is customizable so that it is possible to choose only those analytes that are wanted or needed.
Biological Information
SpecificityCross-reactivity between the antibodies and any of the other analytes in this panel is non-detectable or negligible.
Species Reactivity
  • Human
Analytes Available
  • c-Kit
  • c-Met/HGFR (panTyr)
  • EGFR (pan Tyr)
  • ErbB2/HER2
  • ErbB3/HER3 (panTyr)
  • ErbB4/HER4 (panTyr)
  • FLT3 (pan Tyr)
  • IGF-1R (pan Tyr)
  • M-CSFR (pan Tyr)
  • PDGFR-α (pan Tyr)
  • PDGFRß (pan Tyr)
  • TIE1 (pan Tyr)
  • Tie2 (pan Tyr)
  • VEGFR1 (pan Tyr)
  • VEGFR2 (pan Tyr)
  • VEGFR3 (pan Tyr)
  • FGFR1
Physicochemical Information
Dimensions
Materials Information
Toxicological Information
Safety Information according to GHS
Safety Information
Product Usage Statements
Usage Statement
  • Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
Storage and Shipping Information
Storage ConditionsRecommended storage for kit components is 2 - 8°C.
Packaging Information
Material Size96-well plate
Material Package96-well plate
Transport Information
Supplemental Information
Specifications
Global Trade Item Number
Número de referencia GTIN
HPRTKMAG-01K 04054839033292