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353919 Glutathione Peroxidase Assay Kit

353919
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Descripción

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Detection Methods
Colorimetric

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      Description
      OverviewA sensitive spectrophotometric assay (340 nm) for the measurement of glutathione peroxidase (GPx) activity in plasma, erythrocyte lysates, tissue homogenates, and cell lysates. Assay range: 50-344 nmol/min/ml. Contains sufficient reagents for 96 tests.
      Catalogue Number353919
      Brand Family Calbiochem®
      Materials Required but Not Delivered A plate reader with a 340 nm filter
      Adjustable pipettors and a repeat pipettor
      A source of pure water. Glass distilled water or HPLC-grade water is acceptable
      PBS
      Homogenization buffer (cell and tissue preparation)
      References
      ReferencesUrsini, F., et al. 1985. Biochim. Biophys. Acta 839, 62.
      Forstrom, J.W., et al. 1978. Biochem. 17, 2639.
      Paglia, D.E., et al. 1967. J. Lab. Clin. Med. 70, 158.
      Product Information
      Detection methodColorimetric
      Form96 Tests
      Format96-well plate
      Kit containsAssay Buffer, Sample Buffer, Glutathione Peroxidase (Control), Co-Substrate Mixture, Cumene Hydroperoxide, NADPH, 96 Well Plate, Plate Cover, and a user protocol.
      Positive controlBovine erythrocyte glutathione peroxidase
      Quality LevelMQ100
      Biological Information
      Assay range50-344 nmol/min/ml
      Assay time2 h
      Sample TypeCells, tissues, plasma
      Storage and Shipping Information
      Ship Code Blue Ice Only
      Toxicity Standard Handling
      Storage -20°C
      Storage ConditionsUpon arrival store the entire contents of the kit at -20°C.
      Do not freeze Ok to freeze
      Supplemental Information
      Kit containsAssay Buffer, Sample Buffer, Glutathione Peroxidase (Control), Co-Substrate Mixture, Cumene Hydroperoxide, NADPH, 96 Well Plate, Plate Cover, and a user protocol.
      Global Trade Item Number
      Número de referencia GTIN
      353919-1KIT 04055977193251