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17-10461 EZ-Magna ChIP™ HiSens Chromatin Immunoprecipitation Kit

Single day chromatin immunoprecipitation (ChIP) kit containing all necessary reagents to enable ChIP from low input amounts of chromatin obtained from either cells or tissues using magnetic A/G beads. Control primers included.

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17-10461
24 assays  Kit capacity: 24 chromatin immunoprecipitation assays
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      For target-specific spike-in controls that make ChIP experiments more quantitative and accurate, Click on the Related Product & Applications tab above.
      Description
      Catalogue Number17-10461
      Trade Name
      • Magna ChIP
      DescriptionEZ-Magna ChIP™ HiSens Chromatin Immunoprecipitation Kit
      OverviewThe EZ-Magna HiSens ChIP kit provides a complete set of validated, quality controlled reagents, and a detailed protocol to enable ChIP from a wide range of input amounts of chromatin obtained from either cells or tissues. Our specialized blend of protein A/G blend of magnetic beads is specifically produced for chromatin immunoprecipiptation and enables the use of a broader range of antibodies than protein A or G alone eliminating the need to purchase different kits for different antibody isotypes. The SCW Buffer is unique to the Magna ChIP HiSens kit and enables the use of a single buffer for multiple steps of the ChIP process (sonication, chromatin immunoprecipitation, and wash). The ChIP elution buffer provided with the HiSens kit has been formulated to allow analysis of enrichment by qPCR without additional clean-up steps for more rapid results.

      Features & Benefits
      • Robust ChIP from as few as 10,000 to as many as 1,000,000 cells
      • Protocols and reagents for generation of chromatin from range of input sample type
      • Superior performance with variety of isotypes of either polyclonal or monoclonal antibodies
      • Specialized buffer and bead formulations; lower backgrounds and higher fold enrichment
      • Single buffer system for sonication, chromatin IP, and wash
      • Perform analysis of enrichment without additional purification after cross link reversal.
      • Compatible with all commonly used downstream analysis applications– qPCR, next generation sequencing, microarray
      • Includes positive and negative control antibodies and assay against known positive locus
      Alternate Names
      • Low-input ChIP kit
      • low-input chromatin immunoprecipitation kit
      Materials Required but Not DeliveredReagents
      • Cells, stimulated or treated as desired
      • Antibody of interest for chromatin immunoprecipitation
      • 37% Formaldehyde
      • qPCR reagents, such as SYBR® Green Master Mix
      • 2% Agarose gel
      • 100 bp DNA Ladder
      • 6X gel Loading buffer
      • DNase- and RNase-free sterile H2O (e.g. Millipore Nuclease-free water Cat. No. 3098

      Equipment
      • Microscope and cell counter
      • Magnetic Separator for microcentrifuge tubes
      • (Magna GrIP™ Rack (8 Well), Cat. No. 20-400
      • Vortex mixer
      • Rotating wheel/platform (e.g. Labnet H5500)
      • Microcentrifuge
      • Sonicator
      • Thermomixer® (e.g. Eppendorf 05-400-205)
      • Variable temperature water bath or incubator
      • Timer
      • Variable volume (5-1000 µL) pipettors and tips
      • Cell scraper
      • Microcentrifuge tubes, 1.5 mL (for best results use siliconized tubes)
      • Thermal cycler
      • Real-time PCR Instrument
      • Filter-tip pipette tips
      • Agarose gel electrophoresis equipment or microfluidic electrophoresis system
      Background InformationChromatin Immunoprecipitation (ChIP) is a powerful technique for mapping the in vivo distribution of proteins associated with chromosomal DNA. These proteins can be histone subunits and post-translational modifications or other chromatin associated proteins such as transcription factors, chromatin regulators, etc. Additionally, ChIP can be used to identify regions of the genome associated with these proteins, or conversely, to identify proteins associated with a particular region of the genome. ChIP methodology often involves protein-DNA and protein-protein cross-linking, fragmentation of the cross-linked chromatin, and subsequent immunoprecipitation of chromatin with an antibody specific to a target protein. The DNA fragments isolated in complex with the target protein can be identified by a variety of methods including PCR, DNA microarray and DNA sequencing. Standard or quantitative PCR can be performed to verify whether a particular DNA sequence (the gene or region of the genome) is associated with the protein of interest. The combination of ChIP and promoter or genomic tiling microarrays (ChIP-chip) allows genome-wide identification of DNA-binding sites for chromatin-associated proteins with precise resolution. Alternatively, high-throughput sequencing of libraries constructed from immunoprecipitated chromosomal DNA (ChIP-Seq) is a powerful alternative to ChIP-chip in mapping the protein-DNA interactions across mammalian genomes.
      References
      Product Information
      Components
      • 10X Glycine
      • Nuclei Isolation Buffer
      • 10X PBS
      • SCW Buffer (Sonication/ChIP/Wash)
      • Magna ChIP Protein A/G Magnetic Beads
      • Low Stringency IP Wash Buffer
      • ChIP Elution Buffer
      • Protease Inhibitor Cocktail
      • Proteinase K Solution
      • Anti-Trimethyl-Histone H3 (Lys4)
      • Normal Rabbit IgG
      • Control PCR primers
      PresentationTwo boxes containing all necessary reagents to perform up to 24 chromatin immunoprecipitation reactions. Supplied buffers are sufficient to generate 12 individual chromatin preparation reactions using 15 cm plates of cultured cells or tissue samples.
      Quality LevelMQ100
      Applications
      ApplicationSingle day chromatin immunoprecipitation (ChIP) kit containing all necessary reagents to enable ChIP from low input amounts of chromatin obtained from either cells or tissues using magnetic A/G beads. Control primers included.
      Biological Information
      Analytes Available
      • Protein A+G
      Physicochemical Information
      Dimensions
      Materials Information
      Toxicological Information
      Safety Information according to GHS
      Safety Information
      Product Usage Statements
      Usage Statement
      • Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
      Storage and Shipping Information
      Storage ConditionsUpon receipt, store components at the temperatures indicated on the labels. Kit components are stable for 1 year from date of shipment when stored as directed.
      Packaging Information
      Material Size24 assays
      Material PackageKit capacity: 24 chromatin immunoprecipitation assays
      Transport Information
      Supplemental Information
      Specifications
      Global Trade Item Number
      Número de referencia GTIN
      17-10461 04053252855399

      Documentation

      Licencias necesarias

      Título
      PRODUCTO REGULADO POR LA SECRETARÍA DE SALUD

      EZ-Magna ChIP™ HiSens Chromatin Immunoprecipitation Kit Ficha datos de seguridad (MSDS)

      Título

      Ficha técnica de seguridad del material (MSDS) 

      Folleto

      Cargo
      Magna ChIP™ HiSens Kits
      Magna ChIP™ HiSens Kits

      Licencias necesarias e Información técnica

      Cargo
      EZ-Magna ChIP™ HiSens
      Getting started with ChIP-seq: experimental design to data analysis
      Magna ChIP™ protein A/G bead blends simplify the ChIP process and reduce background signals
      White Paper - The Message in the Marks: Deciphering Cancer Epigenetics